FastQCFastQC Report
Thu 26 May 2016
SRR1545698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545698_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences747880
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34520.4615713750869123No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20110.2688934053591485No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.20096806974380918No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.18679467294218322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC2050.044.025
CGGGTAT207.8547164E-444.06
GGTACGT207.8547164E-444.08
GTCGATA207.8547164E-444.01
GCGAATC207.8547164E-444.025
CGGTTTA254.4413435E-544.028
TAGGTAT302.5265272E-644.06
CTATACG207.8547164E-444.01
CGTTCGA254.4413435E-544.014
ACTACGG254.4413435E-544.02
ATGGTCG600.044.044
TTACGTA207.8547164E-444.01
CGTAAAG454.783942E-1044.02
CGTAAAA254.4413435E-544.032
ATAACGG700.044.02
CATCCGT254.4413435E-544.040
TATAAGT254.4413435E-544.016
TGTCCGA254.4413435E-544.010
CGTTTTA20250.043.6740761
CGTTATT11000.043.01
Produced by FastQC (version 0.11.5)