##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545698_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	747880
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.515672300369044	31.0	31.0	33.0	30.0	34.0
2	31.70014975664545	31.0	31.0	34.0	30.0	34.0
3	31.755514253623577	31.0	31.0	34.0	30.0	34.0
4	35.64774027918917	37.0	35.0	37.0	33.0	37.0
5	27.043141947906083	35.0	25.0	37.0	0.0	37.0
6	31.301965555971545	35.0	26.0	37.0	17.0	37.0
7	15.013413916671123	0.0	0.0	32.0	0.0	37.0
8	25.11184949457132	17.0	17.0	35.0	17.0	37.0
9	33.25638872546398	32.0	32.0	37.0	28.0	39.0
10	36.10498208268706	37.0	35.0	39.0	32.0	39.0
11	37.042857142857144	39.0	37.0	39.0	34.0	39.0
12	36.87192330320372	39.0	35.0	39.0	33.0	39.0
13	36.257076001497566	39.0	35.0	39.0	32.0	39.0
14	37.4756217575012	40.0	35.0	41.0	33.0	41.0
15	37.81406375354335	40.0	36.0	41.0	33.0	41.0
16	37.93335027009681	40.0	35.0	41.0	33.0	41.0
17	37.98280472803123	40.0	35.0	41.0	34.0	41.0
18	37.96900438573033	40.0	36.0	41.0	33.0	41.0
19	37.889253623575975	40.0	36.0	41.0	34.0	41.0
20	37.90065785954966	40.0	36.0	41.0	34.0	41.0
21	37.73791116221854	40.0	35.0	41.0	33.0	41.0
22	37.685849334117776	39.0	35.0	41.0	33.0	41.0
23	37.597529015350055	39.0	35.0	41.0	33.0	41.0
24	37.43778413649248	39.0	35.0	41.0	33.0	41.0
25	37.268870674439746	39.0	35.0	41.0	33.0	41.0
26	37.03341311440338	39.0	35.0	40.0	33.0	41.0
27	36.78482376851901	38.0	35.0	40.0	32.0	41.0
28	36.83541343531048	38.0	35.0	40.0	32.0	41.0
29	36.75387094186233	38.0	35.0	40.0	32.0	41.0
30	36.55456891479916	38.0	35.0	40.0	32.0	41.0
31	36.33136733165748	38.0	35.0	40.0	31.0	41.0
32	36.08871342996203	38.0	35.0	40.0	31.0	41.0
33	35.89280766967963	38.0	35.0	40.0	30.0	41.0
34	35.65148152109964	38.0	35.0	40.0	30.0	41.0
35	35.44790340696368	38.0	34.0	40.0	29.0	41.0
36	35.22201155265551	38.0	34.0	40.0	27.0	41.0
37	35.05972615927689	38.0	34.0	40.0	27.0	41.0
38	34.873041129592984	37.0	34.0	40.0	27.0	41.0
39	34.82683318179387	37.0	34.0	40.0	26.0	41.0
40	34.55455821789592	37.0	34.0	40.0	25.0	40.0
41	34.36521233352944	37.0	34.0	40.0	24.0	40.0
42	34.37567791624325	37.0	33.0	40.0	24.0	40.0
43	34.24274482537305	36.0	33.0	40.0	24.0	40.0
44	34.02960635396053	36.0	33.0	40.0	23.0	40.0
45	33.73420201101781	36.0	33.0	39.0	23.0	40.0
46	33.77346766860994	36.0	33.0	39.0	23.0	40.0
47	33.61340455688078	35.0	33.0	39.0	23.0	40.0
48	33.57916778092742	35.0	33.0	39.0	23.0	40.0
49	33.5003677060491	35.0	33.0	39.0	23.0	40.0
50	33.496297534363805	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	5.0
14	7.0
15	12.0
16	53.0
17	126.0
18	311.0
19	641.0
20	1112.0
21	1931.0
22	3123.0
23	4664.0
24	7000.0
25	9876.0
26	12590.0
27	13883.0
28	14474.0
29	16195.0
30	19982.0
31	26623.0
32	37002.0
33	69436.0
34	67645.0
35	80219.0
36	97258.0
37	169995.0
38	76481.0
39	17234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.375381077178154	22.60777130020859	29.053056640102692	15.963790982510565
2	32.88281542493448	23.4613841792801	31.0589934214045	12.596806974380916
3	29.97352516446489	23.41592234048243	33.93565812697224	12.674894368080441
4	26.124913087661124	26.67205968872012	32.00700647162647	15.196020751992299
5	16.992966786115417	48.10718297053003	23.65967802321228	11.24017222014227
6	20.052682248489063	40.18519013745521	28.974701823822002	10.787425790233728
7	39.02577953682409	55.70572819168851	4.327432208375676	0.9410600631117292
8	87.42445312082152	2.7234315665614806	8.130315023800609	1.7218002888163875
9	83.82882280579771	3.6480451409317003	9.703160934909343	2.8199711183612344
10	55.729796223993155	18.318580520939186	14.63189281702947	11.319730438038187
11	49.150933304808255	18.59362464566508	19.00652511098037	13.24891693854629
12	42.659784992244745	19.30657324704498	24.317804995453816	13.715836765256459
13	23.190083970690484	38.01719527196877	24.979007327378724	13.813713429962027
14	17.18644702358667	39.99612237257314	29.48828689094507	13.329143712895117
15	14.394287853666363	21.019682301973578	49.39522383270044	15.190806011659625
16	17.208241963951437	14.602743755682729	47.44223672246884	20.74677755789699
17	17.3111996576991	15.550355672032948	30.18264962293416	36.955795047333794
18	22.55615874204418	20.506498368722255	35.81978392255442	21.117558966679145
19	31.09362464566508	22.35358613681339	25.62737337540782	20.925415842113708
20	31.503316040006418	22.759801037599615	23.963470075413166	21.7734128469808
21	23.44319944376103	26.391533401080387	29.64178745253249	20.52347970262609
22	23.130849868962937	21.723538535593946	26.602663528908383	28.54294806653474
23	19.843958923891535	29.245868321121037	25.520003209070975	25.390169545916457
24	20.82259185965663	23.25159116435792	38.49334117772904	17.432475798256405
25	18.955714820559447	22.14833930577098	36.39367278172969	22.502273091939884
26	17.162245279991442	31.201663368454835	28.12416430443387	23.51192704711986
27	18.80756271059528	30.30593143285019	30.37679841685832	20.50970743969621
28	16.850965395517996	26.097903406963685	38.81692250093598	18.23420869658234
29	17.14446167834412	23.778012515376798	37.75899876985613	21.318527036422953
30	20.53404289458202	27.37859014815211	33.17042841097502	18.916938546290847
31	31.24017222014227	23.125100283467937	25.37024656361983	20.264480932769963
32	31.3540942397176	24.195191741990694	26.272664063753542	18.17804995453816
33	29.246804300155105	23.574637642402525	25.513451355832483	21.665106701609886
34	20.207252500401136	25.94119377440231	29.141439803176983	24.710113922019573
35	21.33644434936086	23.745253249184362	31.369872172006204	23.548430229448574
36	32.802321228004494	22.109696742792963	25.51786382842167	19.570118200780872
37	21.4840616141627	30.426405305664012	27.673557255174625	20.415975824998664
38	22.587046050168478	31.991495961919025	22.578221104990106	22.843236882922394
39	21.93587206503717	29.046371075573624	25.198427555222764	23.819329304166445
40	25.317965449002518	22.573273787238595	25.726319730438043	26.382441033320852
41	19.12017970797454	21.473097288335026	26.942423918275658	32.46429908541477
42	22.92747499598866	24.41541423757822	24.074049312723965	28.583061453709153
43	22.672621276140557	25.137856340589398	25.40902283788843	26.780499545381613
44	21.31906188158528	28.067604428517946	28.461384179280095	22.151949510616674
45	18.66729956677542	34.623201583141686	23.358292774241857	23.351206075841045
46	23.664224207092047	29.10841311440338	26.69091298069209	20.536449697812483
47	22.635716959940098	26.120099481200192	27.046852436219716	24.197331122639994
48	23.74217788950099	23.596298871476705	30.098144087286734	22.56337915173557
49	22.5330266887736	22.491843611274536	31.715916992030806	23.259212707921055
50	20.937717280847195	30.278386906990427	26.718992351714178	22.0649034604482
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	452.0
1	834.5
2	1217.0
3	5526.5
4	9836.0
5	6957.5
6	4079.0
7	4286.0
8	4493.0
9	4736.0
10	4979.0
11	4987.5
12	4996.0
13	4773.0
14	4550.0
15	4221.5
16	3893.0
17	3557.0
18	3221.0
19	3097.5
20	2974.0
21	2928.5
22	2883.0
23	2987.0
24	3091.0
25	3477.5
26	3864.0
27	4316.5
28	4769.0
29	5494.5
30	6220.0
31	7295.5
32	8371.0
33	9300.0
34	10229.0
35	11643.5
36	13058.0
37	17336.0
38	21614.0
39	31897.0
40	42180.0
41	53693.5
42	65207.0
43	68816.0
44	72425.0
45	70479.0
46	68533.0
47	65064.0
48	61595.0
49	59193.0
50	56791.0
51	52359.5
52	47928.0
53	43316.5
54	38705.0
55	36668.0
56	34631.0
57	33202.5
58	31774.0
59	29709.0
60	27644.0
61	25524.5
62	23405.0
63	20684.5
64	17964.0
65	15350.5
66	12737.0
67	11125.0
68	9513.0
69	8340.5
70	7168.0
71	6053.0
72	4938.0
73	3933.5
74	2929.0
75	2294.0
76	1659.0
77	1185.5
78	712.0
79	551.5
80	391.0
81	285.0
82	179.0
83	113.5
84	48.0
85	35.5
86	23.0
87	15.5
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	747880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.712051279233464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.86829440271188	18.6632546287782
2	7.275138167741854	3.88667727600458
3	3.6443300929255296	2.9204259696204113
4	2.48785360682685	2.6582269248313897
5	1.9147727617407078	2.557375409984863
6	1.540950642968983	2.469717155625315
7	1.3206062141639376	2.4693270638695033
8	1.121249904220428	2.3960707950697344
9	0.9664838217052515	2.323508886534619
>10	9.473865371384885	50.634535279705325
>50	0.3340898356140466	5.667162517462902
>100	0.05027057087586121	2.1839172747083873
>500	0.0	0.0
>1k	0.0020946071198275507	1.1698008178047938
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3452	0.4615713750869123	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2011	0.2688934053591485	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1503	0.20096806974380918	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1397	0.18679467294218322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009226079050114992	0.0
2	0.0	0.0	0.0	0.03516606942290207	0.0
3	0.0	0.0	0.0	0.04653152912231909	0.0
4	0.0	0.0	0.0	0.06378028560731668	0.0
5	0.0	0.0	0.0	0.11298604054126331	0.0
6	0.0	0.0	0.0	0.2007006471626464	0.0
7	0.0	0.0	0.0	0.2598010375996149	0.0
8	0.0	0.0	0.0	0.5808418462855004	0.0
9	0.0	0.0	0.0	0.7219072578488528	0.0
10	0.0	0.0	0.0	0.9718136599454458	0.0
11	0.0	0.0	0.0	1.1658287425790235	0.0
12	0.0	0.0	0.0	1.3351072364550463	0.0
13	0.0	0.0	0.0	1.4121249398299192	0.0
14	0.0	0.0	0.0	1.4391346205273574	0.0
15	0.0	0.0	0.0	1.481253677060491	0.0
16	0.0	0.0	0.0	1.5697705514253624	0.0
17	0.0	0.0	0.0	1.6791463871209285	0.0
18	0.0	0.0	0.0	1.8520350858426486	0.0
19	0.0	0.0	0.0	1.9119377440231053	0.0
20	0.0	0.0	0.0	1.984944108680537	0.0
21	0.0	0.0	0.0	2.0745306733700595	0.0
22	0.0	0.0	0.0	2.1635823928972564	0.0
23	0.0	0.0	0.0	2.2779055463443334	0.0
24	0.0	0.0	0.0	2.362945927154089	0.0
25	0.0	0.0	0.0	2.420040648232337	0.0
26	0.0	0.0	0.0	2.493314435470931	0.0
27	0.0	0.0	0.0	2.5700647162646413	0.0
28	0.0	0.0	0.0	2.6457453067337005	0.0
29	0.0	0.0	0.0	2.7333262020645024	0.0
30	0.0	0.0	0.0	2.839626678076697	0.0
31	0.0	0.0	0.0	2.9375033427822643	0.0
32	0.0	0.0	0.0	3.0245493929507408	0.0
33	0.0	0.0	0.0	3.115874204417821	0.0
34	0.0	0.0	0.0	3.211745199764668	0.0
35	0.0	0.0	0.0	3.3371663903299993	0.0
36	0.0	0.0	0.0	3.438920682462427	0.0
37	0.0	0.0	0.0	3.5309140503824143	0.0
38	0.0	0.0	0.0	3.6488474086751888	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	205	0.0	44.0	25
CGGGTAT	20	7.8547164E-4	44.0	6
GGTACGT	20	7.8547164E-4	44.0	8
GTCGATA	20	7.8547164E-4	44.0	1
GCGAATC	20	7.8547164E-4	44.0	25
CGGTTTA	25	4.4413435E-5	44.0	28
TAGGTAT	30	2.5265272E-6	44.0	6
CTATACG	20	7.8547164E-4	44.0	1
CGTTCGA	25	4.4413435E-5	44.0	14
ACTACGG	25	4.4413435E-5	44.0	2
ATGGTCG	60	0.0	44.0	44
TTACGTA	20	7.8547164E-4	44.0	1
CGTAAAG	45	4.783942E-10	44.0	2
CGTAAAA	25	4.4413435E-5	44.0	32
ATAACGG	70	0.0	44.0	2
CATCCGT	25	4.4413435E-5	44.0	40
TATAAGT	25	4.4413435E-5	44.0	16
TGTCCGA	25	4.4413435E-5	44.0	10
CGTTTTA	2025	0.0	43.674076	1
CGTTATT	1100	0.0	43.0	1
>>END_MODULE