FastQCFastQC Report
Thu 26 May 2016
SRR1545697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545697_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences287309
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.3028098667288529No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.29932929354806154No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7520.26173910319551424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCAA302.159799E-645.0000041
ACGGGCT302.159799E-645.0000045
TGGTTCG302.159799E-645.00000437
GGCTTAT302.159799E-645.00000445
ATATCAC302.159799E-645.00000430
TAGGATG700.045.0000046
AGGATCA453.8198777E-1045.0000047
TGGGACC302.159799E-645.0000046
CAATAAG302.159799E-645.0000042
GTAGTAA302.159799E-645.0000041
TAATCAC207.024446E-445.016
TGATAAA207.024446E-445.01
TTGGAAT207.024446E-445.032
GATCGAC207.024446E-445.09
TATGGTA207.024446E-445.01
ATTAACA253.883479E-545.023
TCTAGTC207.024446E-445.030
TGTGACG207.024446E-445.043
ACAGGTA253.883479E-545.05
AGGCAAT253.883479E-545.07