FastQCFastQC Report
Thu 26 May 2016
SRR1545696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545696_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences996029
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47720.479102516091399No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.21826673721347473No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.20682128733199534No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17090.17158134953901943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA302.5273748E-644.01
TACGATT302.5273748E-644.013
TGATTCG408.310963E-944.015
CTACCTG254.4424112E-544.033
CGTAAAT351.4458237E-744.016
TTACGCA254.4424112E-544.01
ATCGCGA207.855975E-444.01
CGTTTTA28850.043.6187171
CGTTATT14600.042.493151
CGGTCTA2550.042.2745131
GTTTTAT33150.041.6108632
CGACGGT2600.041.4615428
CGTTCGA750.041.06666614
TTCGAAT750.041.06666616
ACGGTCT2650.040.67924530
CACTCGC650.040.61538722
TCACGAC2750.040.025
ATGCTTG7150.040.036
CACGACG2750.040.026
TATGCCA2750.040.010
Produced by FastQC (version 0.11.5)