##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545696_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	996029
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49639016534659	31.0	31.0	33.0	30.0	34.0
2	31.688740990473168	31.0	31.0	34.0	30.0	34.0
3	31.728139441723084	31.0	31.0	34.0	30.0	34.0
4	35.64315998831359	37.0	35.0	37.0	33.0	37.0
5	27.115136205873522	35.0	25.0	37.0	0.0	37.0
6	31.33771607051602	35.0	26.0	37.0	17.0	37.0
7	14.977252670353975	0.0	0.0	32.0	0.0	37.0
8	25.068089382939654	17.0	17.0	35.0	17.0	37.0
9	33.11173871443502	32.0	32.0	37.0	28.0	39.0
10	35.98318020860838	37.0	35.0	39.0	32.0	39.0
11	36.98698431471373	39.0	37.0	39.0	34.0	39.0
12	37.00832505880853	39.0	35.0	39.0	33.0	39.0
13	36.52217555914537	39.0	35.0	39.0	33.0	39.0
14	37.863195750324536	40.0	36.0	41.0	33.0	41.0
15	38.13166684905761	40.0	36.0	41.0	33.0	41.0
16	38.19976325990508	40.0	36.0	41.0	34.0	41.0
17	38.21742238428801	40.0	36.0	41.0	34.0	41.0
18	38.20293786626694	40.0	36.0	41.0	34.0	41.0
19	38.13654421708605	40.0	36.0	41.0	34.0	41.0
20	38.143744810643064	40.0	36.0	41.0	34.0	41.0
21	37.989566568844886	40.0	36.0	41.0	34.0	41.0
22	37.887830575214174	40.0	35.0	41.0	33.0	41.0
23	37.77352767841097	40.0	35.0	41.0	33.0	41.0
24	37.60947623010976	39.0	35.0	41.0	33.0	41.0
25	37.423138282118295	39.0	35.0	41.0	33.0	41.0
26	37.14925971030964	39.0	35.0	40.0	33.0	41.0
27	36.94087119953335	39.0	35.0	40.0	32.0	41.0
28	36.94335405896816	39.0	35.0	40.0	32.0	41.0
29	36.890342550267114	39.0	35.0	40.0	32.0	41.0
30	36.69936417513948	38.0	35.0	40.0	32.0	41.0
31	36.51303827498999	38.0	35.0	40.0	31.0	41.0
32	36.26021029508177	38.0	35.0	40.0	31.0	41.0
33	36.03004731789938	38.0	35.0	40.0	30.0	41.0
34	35.77951947182261	38.0	35.0	40.0	30.0	41.0
35	35.61090189141079	38.0	35.0	40.0	29.0	41.0
36	35.407998160696124	38.0	35.0	40.0	27.0	41.0
37	35.26236786278311	38.0	34.0	40.0	27.0	41.0
38	35.080868127333645	38.0	34.0	40.0	27.0	41.0
39	35.0251087066742	38.0	34.0	40.0	26.0	41.0
40	34.79116772704409	38.0	34.0	40.0	25.0	41.0
41	34.582623598308885	37.0	34.0	40.0	24.0	40.0
42	34.58114773766627	37.0	34.0	40.0	24.0	40.0
43	34.43934162559524	37.0	33.0	40.0	24.0	40.0
44	34.23271109576127	37.0	33.0	40.0	23.0	40.0
45	34.00464845903081	36.0	33.0	40.0	23.0	40.0
46	33.974663388315	36.0	33.0	39.0	23.0	40.0
47	33.78331755400696	36.0	33.0	39.0	23.0	40.0
48	33.697963613509245	36.0	33.0	39.0	23.0	40.0
49	33.54313478824412	36.0	33.0	39.0	23.0	40.0
50	33.66056108808077	36.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	3.0
14	4.0
15	19.0
16	61.0
17	148.0
18	436.0
19	803.0
20	1562.0
21	2631.0
22	4103.0
23	6325.0
24	9285.0
25	13568.0
26	17040.0
27	18355.0
28	18566.0
29	20540.0
30	24662.0
31	32346.0
32	44909.0
33	80817.0
34	83684.0
35	103418.0
36	134022.0
37	243882.0
38	108286.0
39	26550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.32506282447599	23.785552428694345	27.52490138339346	16.364483363436204
2	34.98271636669214	24.342865518975852	28.08090929079374	12.593508823538272
3	30.83384118333904	23.366387926455957	33.21198479160748	12.587786098597531
4	27.073709701223557	26.57994897738921	31.406615670828863	14.939725650558366
5	17.377907671362983	47.62722772128121	23.905127260350852	11.089737347004956
6	20.634640156059714	40.25003288056874	28.79604911101986	10.319277852351687
7	38.03543872718566	56.124871866180605	4.676470263416025	1.1632191432177177
8	84.91961579431924	3.748987228283514	9.216297919036494	2.1150990583607503
9	80.4148272791254	5.547529238606506	10.86092874805854	3.176714734209546
10	48.00081122136002	23.08436802542898	17.022195136888584	11.892625616322416
11	42.701266730185566	21.3371297422063	21.91191220335954	14.049691324248592
12	39.335300478198924	20.124514446868517	26.401841713444085	14.13834336148847
13	22.530167294325768	35.92194604775564	25.546244135461922	16.001642522456674
14	15.748336644816566	40.02503943158281	28.824562337040387	15.402061586560231
15	14.982595888272328	23.289582933830243	45.67176256916214	16.056058608735288
16	16.06449209812164	17.91785178945593	46.042434507429	19.97522160499343
17	16.28055006430536	18.160816602729437	31.817547481047235	33.741085851917965
18	20.21256409200937	23.171212886371784	34.48092374820412	22.13529927341473
19	28.8065909727528	24.085142099276226	26.629043933459766	20.479222994511204
20	31.0700792848401	22.26702234573491	26.265600700381214	20.397297669043773
21	22.805259686213954	26.994695937568082	28.769041865246898	21.431002510971066
22	22.260496431328807	24.195982245496868	26.56348359334919	26.980037729825135
23	21.443451947684256	28.768740669197385	25.86089360851943	23.926913774598933
24	20.33394609996295	24.131124696168484	37.39820828510013	18.13672091876843
25	19.806351019900024	24.422381276047183	34.89928506097714	20.871982643075654
26	17.767554960749134	32.6649123670094	28.440336576545462	21.127196095696007
27	19.891589501912094	30.83705394120051	29.009396312757964	20.261960244129437
28	17.738439342629583	27.881818702065903	36.548534229425044	17.831207725879466
29	17.98953644923993	24.695666491638296	36.164609664979636	21.15018739414214
30	19.778741382028034	27.766661412468913	32.35307405708067	20.101523148422384
31	27.95721811312723	26.233573520449703	25.761298114813926	20.047910251609142
32	29.348141469776483	26.068216889267283	26.68105045134228	17.902591189613958
33	27.198103669672268	26.75725305186897	25.459600071885458	20.585043206573303
34	20.46586996964948	27.02672311749959	28.225182198510286	24.282224714340646
35	21.094265327615965	24.81624531012651	30.330040591187608	23.75944877106992
36	28.14305607567651	25.59543949021565	25.956975148313955	20.304529285793887
37	21.263637906125226	30.92450119424234	27.983723365484337	19.8281375341481
38	20.770780770439416	31.552093362743456	25.09143810069787	22.585687766119257
39	21.41644470191129	30.151230536460282	25.852058524400395	22.580266237228034
40	23.46578262279512	25.65256634093987	27.934929605463292	22.946721430801713
41	18.776461327933223	25.024371780339727	28.291445329403057	27.90772156232399
42	22.534484437702115	27.710839744625908	24.198391813892968	25.556284003779005
43	22.76901576158927	27.075617276203808	25.71581751133752	24.439549450869404
44	21.088341805308882	29.624137449813208	28.187231496271696	21.100289248606217
45	18.528777776550683	34.0618596446489	24.237045306913753	23.172317271886662
46	21.93048595974615	31.306819379757016	26.145825071358363	20.61686958913847
47	22.628658402516393	28.40007670459394	26.327145093164955	22.644119799724706
48	23.250327048710428	25.16623511966017	29.016123024530412	22.567314807098988
49	21.259220364065705	25.250268817474193	30.55403005334182	22.936480765118283
50	19.776030617582418	30.568387065035257	27.492271811362922	22.163310506019403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	944.0
1	1335.5
2	1727.0
3	8300.5
4	14874.0
5	10979.0
6	7084.0
7	7106.5
8	7129.0
9	7274.5
10	7420.0
11	7337.0
12	7254.0
13	6874.0
14	6494.0
15	6177.0
16	5860.0
17	5460.5
18	5061.0
19	4950.0
20	4839.0
21	5094.0
22	5349.0
23	5647.0
24	5945.0
25	7046.5
26	8148.0
27	9762.5
28	11377.0
29	13505.0
30	15633.0
31	16936.5
32	18240.0
33	19979.0
34	21718.0
35	22798.0
36	23878.0
37	27920.0
38	31962.0
39	43082.0
40	54202.0
41	67880.0
42	81558.0
43	86703.5
44	91849.0
45	90699.0
46	89549.0
47	84252.0
48	78955.0
49	73857.0
50	68759.0
51	62892.0
52	57025.0
53	52887.0
54	48749.0
55	46231.5
56	43714.0
57	42977.5
58	42241.0
59	39879.0
60	37517.0
61	32380.0
62	27243.0
63	22855.5
64	18468.0
65	15858.0
66	13248.0
67	11841.0
68	10434.0
69	9377.5
70	8321.0
71	7114.5
72	5908.0
73	4652.5
74	3397.0
75	2636.5
76	1876.0
77	1448.5
78	1021.0
79	795.0
80	569.0
81	427.5
82	286.0
83	205.0
84	124.0
85	83.0
86	42.0
87	32.0
88	22.0
89	15.5
90	9.0
91	6.5
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	996029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.867152996357095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.95266307758013	18.09476238330385
2	7.1597345202982625	3.7040389649970904
3	3.5245450174802677	2.735098356291305
4	2.367003293136349	2.449105453057561
5	1.887420838697216	2.4411101801546753
6	1.5457659248623394	2.399073820498174
7	1.253540037993065	2.269785836487813
8	1.1118029205564555	2.300734099826439
9	0.9771551598801004	2.2748599819618502
>10	9.774130569465257	51.466620613478966
>50	0.39703361970206597	6.533601736618304
>100	0.046659933089115535	2.0177073272350046
>500	8.483624198021003E-4	0.13781284479891048
>1k	0.0016967248396042005	1.1756884012900684
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4772	0.479102516091399	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2174	0.21826673721347473	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2060	0.20682128733199534	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1709	0.17158134953901943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008734685435865823	0.0
2	0.0	0.0	0.0	0.03242877466419151	0.0
3	0.0	0.0	0.0	0.04648459030811352	0.0
4	0.0	0.0	0.0	0.06917469270473048	0.0
5	0.0	0.0	0.0	0.12690393552798163	0.0
6	0.0	0.0	0.0	0.23684049360008594	0.0
7	0.0	0.0	0.0	0.3104327283643348	0.0
8	0.0	0.0	0.0	0.6769883206211867	0.0
9	0.0	0.0	0.0	0.8474652846453266	0.0
10	0.0	0.0	0.0	1.1302883751376718	0.0
11	0.0	0.0	0.0	1.32937896386551	0.0
12	0.0	0.0	0.0	1.5131085540682048	0.0
13	0.0	0.0	0.0	1.5923231150900226	0.0
14	0.0	0.0	0.0	1.6243502950215305	0.0
15	0.0	0.0	0.0	1.6643089709235375	0.0
16	0.0	0.0	0.0	1.7554709752426887	0.0
17	0.0	0.0	0.0	1.8557692597303894	0.0
18	0.0	0.0	0.0	2.010684427863044	0.0
19	0.0	0.0	0.0	2.0716264285477632	0.0
20	0.0	0.0	0.0	2.145218663312012	0.0
21	0.0	0.0	0.0	2.2213208651555325	0.0
22	0.0	0.0	0.0	2.309370510296387	0.0
23	0.0	0.0	0.0	2.4071588277048157	0.0
24	0.0	0.0	0.0	2.4850682058454123	0.0
25	0.0	0.0	0.0	2.541191069737929	0.0
26	0.0	0.0	0.0	2.605546625650458	0.0
27	0.0	0.0	0.0	2.6730145407412835	0.0
28	0.0	0.0	0.0	2.7428920242282104	0.0
29	0.0	0.0	0.0	2.8174882458241677	0.0
30	0.0	0.0	0.0	2.9130677922028374	0.0
31	0.0	0.0	0.0	3.0014186333932042	0.0
32	0.0	0.0	0.0	3.0777216326030667	0.0
33	0.0	0.0	0.0	3.1554302133773215	0.0
34	0.0	0.0	0.0	3.2359499572803605	0.0
35	0.0	0.0	0.0	3.349500867946616	0.0
36	0.0	0.0	0.0	3.4435744340777226	0.0
37	0.0	0.0	0.0	3.5405595620207846	0.0
38	0.0	0.0	0.0	3.6294123966270058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	30	2.5273748E-6	44.0	1
TACGATT	30	2.5273748E-6	44.0	13
TGATTCG	40	8.310963E-9	44.0	15
CTACCTG	25	4.4424112E-5	44.0	33
CGTAAAT	35	1.4458237E-7	44.0	16
TTACGCA	25	4.4424112E-5	44.0	1
ATCGCGA	20	7.855975E-4	44.0	1
CGTTTTA	2885	0.0	43.618717	1
CGTTATT	1460	0.0	42.49315	1
CGGTCTA	255	0.0	42.27451	31
GTTTTAT	3315	0.0	41.610863	2
CGACGGT	260	0.0	41.46154	28
CGTTCGA	75	0.0	41.066666	14
TTCGAAT	75	0.0	41.066666	16
ACGGTCT	265	0.0	40.679245	30
CACTCGC	65	0.0	40.615387	22
TCACGAC	275	0.0	40.0	25
ATGCTTG	715	0.0	40.0	36
CACGACG	275	0.0	40.0	26
TATGCCA	275	0.0	40.0	10
>>END_MODULE