FastQCFastQC Report
Thu 26 May 2016
SRR1545695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545695_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences383665
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.3211134713877992No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.309123845021047No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6800.1772379549867723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC207.0274057E-445.06
CGACGGT750.045.028
CCGATGT207.0274057E-445.011
GCGAATA207.0274057E-445.01
AACCCGA207.0274057E-445.024
CACGACG750.045.026
TAACGCC253.8859303E-545.012
TATGCAC253.8859303E-545.015
ACGGGAT406.7939254E-945.05
TAACCGG207.0274057E-445.016
AACGCCG253.8859303E-545.013
AGCGCAA207.0274057E-445.014
ACGCTTG207.0274057E-445.032
AGCGAAA406.7939254E-945.01
CGTAAAT207.0274057E-445.016
CGATGAT207.0274057E-445.010
CGGTCTA750.045.031
ACGTAAG207.0274057E-445.02
CGACTAG351.2091004E-745.02
CGGTAAG207.0274057E-445.02