##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545695_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	383665
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96662974209271	31.0	31.0	33.0	30.0	33.0
2	31.127952771297878	31.0	31.0	33.0	30.0	34.0
3	31.056979396087733	31.0	31.0	34.0	28.0	34.0
4	35.022868909074326	35.0	35.0	37.0	32.0	37.0
5	32.74656796945252	35.0	35.0	37.0	28.0	37.0
6	33.935646983696714	35.0	35.0	37.0	28.0	37.0
7	15.386128523581771	0.0	0.0	35.0	0.0	37.0
8	24.932245578825277	17.0	17.0	35.0	17.0	37.0
9	32.62214431861129	32.0	32.0	37.0	27.0	39.0
10	35.42336152633157	37.0	34.0	37.0	32.0	39.0
11	36.40242659611901	37.0	35.0	39.0	32.0	39.0
12	36.3002254571045	37.0	35.0	39.0	32.0	39.0
13	35.96766710541748	37.0	35.0	39.0	31.0	39.0
14	37.015542204788034	39.0	35.0	40.0	31.0	41.0
15	37.35747331656523	39.0	36.0	41.0	32.0	41.0
16	37.4310192485632	39.0	35.0	41.0	32.0	41.0
17	37.37732657396427	39.0	35.0	41.0	32.0	41.0
18	37.296959587139824	39.0	36.0	40.0	32.0	41.0
19	37.22505049978497	39.0	36.0	40.0	32.0	41.0
20	37.31077632830725	39.0	35.0	40.0	32.0	41.0
21	37.14451148788657	39.0	35.0	40.0	32.0	41.0
22	37.18036047072316	39.0	35.0	40.0	32.0	41.0
23	37.14673478164545	39.0	35.0	40.0	32.0	41.0
24	36.96468012458786	39.0	35.0	40.0	32.0	41.0
25	36.697092515606066	38.0	35.0	40.0	31.0	41.0
26	36.55309970938188	38.0	35.0	40.0	31.0	41.0
27	36.45045547547991	38.0	35.0	40.0	31.0	41.0
28	36.3412299792788	38.0	35.0	40.0	30.0	41.0
29	36.32917258545867	38.0	35.0	40.0	30.0	41.0
30	36.08435223437113	38.0	35.0	40.0	30.0	41.0
31	35.932886763191846	38.0	35.0	40.0	30.0	41.0
32	35.81800529107424	38.0	34.0	40.0	29.0	41.0
33	35.591966950334275	38.0	34.0	40.0	28.0	41.0
34	35.41873770085882	38.0	34.0	40.0	27.0	41.0
35	35.232205178997305	38.0	34.0	40.0	26.0	41.0
36	35.014197281482545	38.0	34.0	40.0	25.0	41.0
37	34.869500736319445	38.0	34.0	40.0	24.0	41.0
38	34.75421526592209	38.0	34.0	40.0	24.0	41.0
39	34.67657461587583	38.0	33.0	40.0	23.0	41.0
40	34.525218615198156	38.0	33.0	40.0	23.0	41.0
41	34.34808231139145	37.0	33.0	40.0	23.0	41.0
42	34.30014205100804	37.0	33.0	40.0	23.0	41.0
43	34.208843652665735	37.0	33.0	40.0	23.0	41.0
44	33.98306334953671	37.0	33.0	40.0	22.0	41.0
45	33.892049053210485	37.0	33.0	40.0	22.0	41.0
46	33.873647583178034	37.0	33.0	40.0	22.0	41.0
47	33.796168532443666	36.0	33.0	40.0	22.0	41.0
48	33.743494976085906	36.0	33.0	40.0	22.0	41.0
49	33.608958336048374	36.0	33.0	40.0	20.0	41.0
50	33.47301682457352	36.0	32.0	40.0	20.0	41.0
51	33.28829577886959	36.0	32.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	7.0
15	20.0
16	51.0
17	126.0
18	292.0
19	565.0
20	975.0
21	1503.0
22	2379.0
23	3439.0
24	4656.0
25	6113.0
26	7270.0
27	7787.0
28	8337.0
29	9597.0
30	11335.0
31	14516.0
32	19215.0
33	28064.0
34	35906.0
35	38215.0
36	44480.0
37	67381.0
38	59444.0
39	11989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.886932610480496	24.636336387212804	27.721319380188447	15.755411622118254
2	34.3789503864048	24.309749390744532	28.576753157051073	12.734547065799589
3	31.50222199053862	23.064652756962452	32.65922093493021	12.773904317568713
4	27.57301291491275	27.09473108049992	30.128627839391136	15.2036281651962
5	21.41842492799708	36.06036516231608	28.80898700689404	13.712222902792801
6	20.642748230878503	41.179674976867844	28.157376878266195	10.020199913987463
7	37.2919083054227	56.833696062971605	5.020004430948875	0.854391200656823
8	85.47613152098836	4.054578864373868	8.418281573768782	2.0510080408689872
9	80.79704950933757	5.729738183050317	10.070243571866081	3.4029687357460285
10	48.28457117537435	23.576818318063935	16.326743382899146	11.81186712366257
11	43.408442260826504	21.639711727679096	21.14057836915017	13.811267642344232
12	39.81911302829291	20.508777188432617	25.6455501544316	14.026559628842872
13	22.76360887753639	36.749508034352885	24.64233120039618	15.844551887714541
14	15.51822553529772	41.1014817614325	28.052597969582838	15.327694733686942
15	14.876259236573574	23.786897423533553	45.323133462786544	16.01370987710633
16	15.967315235948027	18.215891467816974	45.86344858144475	19.953344714790248
17	16.153415088684138	18.592522122163867	31.223072211434456	34.03099057771754
18	20.147785177172793	23.43059700519985	34.204579515983994	22.21703830164336
19	29.569285704977	24.06005238945434	26.02218080877849	20.34848109679017
20	31.76416926224701	22.41486713669477	25.569181447356414	20.251782153701797
21	22.91009083445193	27.645471961216167	28.095082949969374	21.34935425436253
22	22.15031342447187	24.576127611327593	25.861884717136043	27.411674247064493
23	21.387668929925844	29.256512843235633	25.30749481969948	24.04832340713904
24	20.283059439875935	24.42391148528013	37.39069240092268	17.90233667392126
25	19.76568099774543	24.74163658399906	34.90310557387304	20.589576844382467
26	17.675576349158774	33.279293133332466	28.185265791771467	20.8598647257373
27	19.77089387877445	31.89709772848709	28.228532704312354	20.1034756884261
28	17.706332347230006	28.58796085126347	36.10467465106277	17.601032150443746
29	17.864543286460847	25.195417877575487	35.927436696076	21.01260213988766
30	19.6900942228246	28.705250674416483	31.58510679890008	20.019548303858837
31	28.287438259940313	26.63964656666623	25.187598556031954	19.8853166173615
32	30.024630862862132	26.176482087237563	26.100634668265283	17.698252381635022
33	27.928531401092098	26.807761979852213	24.791680241877682	20.472026377178008
34	20.570810472677987	27.167711414906233	27.52062346057107	24.740854651844707
35	21.283671953396844	24.781775767922536	30.263380813991375	23.671171464689248
36	28.66797857505897	25.918183832249486	25.369006815841942	20.044830776849594
37	21.239101820598698	31.776940820768118	27.514368003336244	19.469589355296936
38	20.588273624125215	32.38319888444346	24.746849465028085	22.28167802640324
39	21.492969126712104	30.76485996898336	25.443290370505515	22.298880533799018
40	23.86013840199132	25.743031029674324	27.632439758643606	22.764390809690745
41	18.53596236299897	25.269962076290515	28.01428329401952	28.179792266690995
42	22.384893070777892	27.678573755750456	23.99801910520897	25.938514068262673
43	22.64136681740581	26.906806719403647	25.632517951859047	24.8193085113315
44	20.830672591974768	29.679277494689376	28.450601436148727	21.03944847718713
45	18.385310101260213	34.97217624750759	23.919043957619277	22.72346969361292
46	21.834152190061644	31.972163215305017	25.697157676618925	20.496526918014414
47	22.395318832835937	28.774321348051036	25.896550375979043	22.933809443133985
48	23.00209818461418	25.57465497243689	29.223671692752788	22.199575150196136
49	21.30400218941003	25.72426465796984	30.50499784968658	22.46673530293355
50	19.77740998006073	31.596575137163935	26.885955195287554	21.740059687487783
51	19.236834217351074	32.90657213975734	25.03929209075626	22.817301552135326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	386.0
1	520.5
2	655.0
3	2614.5
4	4574.0
5	3486.5
6	2399.0
7	2407.0
8	2415.0
9	2439.5
10	2464.0
11	2475.0
12	2486.0
13	2407.0
14	2328.0
15	2200.5
16	2073.0
17	1953.0
18	1833.0
19	1791.0
20	1749.0
21	1784.5
22	1820.0
23	1962.0
24	2104.0
25	2542.5
26	3457.5
27	3934.0
28	4673.5
29	5413.0
30	6075.0
31	6737.0
32	7690.0
33	8643.0
34	9019.5
35	9396.0
36	10166.0
37	10936.0
38	14641.0
39	18346.0
40	23771.5
41	29197.0
42	31811.5
43	34426.0
44	33915.0
45	33404.0
46	32576.0
47	31748.0
48	29180.5
49	26613.0
50	25214.5
51	23816.0
52	21914.5
53	20013.0
54	18879.0
55	17745.0
56	17111.0
57	16477.0
58	15669.5
59	14862.0
60	13578.5
61	12295.0
62	10334.5
63	8374.0
64	7064.5
65	5755.0
66	5021.5
67	4288.0
68	3938.5
69	3589.0
70	3221.5
71	2854.0
72	2475.0
73	2096.0
74	1621.0
75	871.0
76	596.0
77	449.5
78	303.0
79	255.0
80	207.0
81	152.0
82	97.0
83	78.5
84	60.0
85	38.5
86	17.0
87	13.0
88	9.0
89	6.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	383665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.40805046874119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.37164811644958	25.57650063371159
2	8.906914961157188	6.663806487755081
3	4.820980643948486	5.410304617129482
4	3.34305925592799	5.002293174629865
5	2.532659274115935	4.737092297312715
6	1.9831767101492468	4.451206467701708
7	1.6070561437135835	4.208178613109682
8	1.3454754788503347	4.026529169382963
9	1.1606458050290254	3.9075747165772667
>10	5.871203647597771	33.42575768294952
>50	0.042712743491240164	1.08418492787094
>100	0.01240047391681166	0.7081863992843695
>500	6.889152176006478E-4	0.17524263155508418
>1k	0.0013778304352012956	0.6231421810296964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1232	0.3211134713877992	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1186	0.309123845021047	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	680	0.1772379549867723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007558677492082937	0.0
2	0.0	0.0	0.0	0.03961789582057264	0.0
3	0.0	0.0	0.0	0.04926172572426466	0.0
4	0.0	0.0	0.0	0.07532613086937823	0.0
5	0.0	0.0	0.0	0.13449233054878604	0.0
6	0.0	0.0	0.0	0.20955781736671314	0.0
7	0.0	0.0	0.0	0.2510002215474437	0.0
8	0.0	0.0	0.0	0.5619485749286487	0.0
9	0.0	0.0	0.0	0.7071273115869313	0.0
10	0.0	0.0	0.0	0.9531752961568034	0.0
11	0.0	0.0	0.0	1.1158171842623121	0.0
12	0.0	0.0	0.0	1.266990734103971	0.0
13	0.0	0.0	0.0	1.3384072042015822	0.0
14	0.0	0.0	0.0	1.362907745037989	0.0
15	0.0	0.0	0.0	1.395488251469381	0.0
16	0.0	0.0	0.0	1.472378246647466	0.0
17	0.0	0.0	0.0	1.559172715780694	0.0
18	0.0	0.0	0.0	1.7095643334679995	0.0
19	0.0	0.0	0.0	1.7666453807357982	0.0
20	0.0	0.0	0.0	1.8255509363637548	0.0
21	0.0	0.0	0.0	1.8961854743070126	0.0
22	0.0	0.0	0.0	1.9712509611249398	0.0
23	0.0	0.0	0.0	2.057784786206717	0.0
24	0.0	0.0	0.0	2.131286408715937	0.0
25	0.0	0.0	0.0	2.194362269167112	0.0
26	0.0	0.0	0.0	2.250661384280557	0.0
27	0.0	0.0	0.0	2.311652092320123	0.0
28	0.0	0.0	0.0	2.362738326404546	0.0
29	0.0	0.0	0.0	2.4239896784955626	0.0
30	0.0	0.0	0.0	2.5102628595258887	0.0
31	0.0	0.0	0.0	2.588456074961229	0.0
32	0.0	0.0	0.0	2.6559628842870735	0.0
33	0.0	0.0	0.0	2.728161286539038	0.0
34	0.0	0.0	0.0	2.8045299936142207	0.0
35	0.0	0.0	0.0	2.9025321569598477	0.0
36	0.0	0.0	0.0	2.9804647283437373	0.0
37	0.0	0.0	0.0	3.062828248602296	0.0
38	0.0	0.0	0.0	3.1420640402434414	0.0
39	0.0	0.0	0.0	3.221821119987489	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	20	7.0274057E-4	45.0	6
CGACGGT	75	0.0	45.0	28
CCGATGT	20	7.0274057E-4	45.0	11
GCGAATA	20	7.0274057E-4	45.0	1
AACCCGA	20	7.0274057E-4	45.0	24
CACGACG	75	0.0	45.0	26
TAACGCC	25	3.8859303E-5	45.0	12
TATGCAC	25	3.8859303E-5	45.0	15
ACGGGAT	40	6.7939254E-9	45.0	5
TAACCGG	20	7.0274057E-4	45.0	16
AACGCCG	25	3.8859303E-5	45.0	13
AGCGCAA	20	7.0274057E-4	45.0	14
ACGCTTG	20	7.0274057E-4	45.0	32
AGCGAAA	40	6.7939254E-9	45.0	1
CGTAAAT	20	7.0274057E-4	45.0	16
CGATGAT	20	7.0274057E-4	45.0	10
CGGTCTA	75	0.0	45.0	31
ACGTAAG	20	7.0274057E-4	45.0	2
CGACTAG	35	1.2091004E-7	45.0	2
CGGTAAG	20	7.0274057E-4	45.0	2
>>END_MODULE