FastQCFastQC Report
Thu 26 May 2016
SRR1545694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545694_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1433951
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.36486602401337287No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21480.149795913528426No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20440.14254322497770147No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20390.1421945380281474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA207.857134E-444.00000411
TAACGCC408.314601E-944.00000412
CGCGAAA207.857134E-444.00000427
CCGTAGT207.857134E-444.00000428
CGCTAAA207.857134E-444.00000420
TACGTAA650.044.0000041
TATCACG302.5281588E-644.01
AACCGTA302.5281588E-644.010
TCGTTAA254.4433946E-544.01
GGCGTAA254.4433946E-544.01
TAGCGAT254.4433946E-544.06
AACGGTC351.446424E-744.021
CGTTAAG1350.044.02
CGAATCG302.5281588E-644.026
CCGCGAA254.4433946E-544.023
CGTTTTA35850.042.7112921
CGGTCTA3000.042.53333331
CGTTATT20750.042.197591
CGACGGT2950.041.7627128
TAACGGG2850.040.912283
Produced by FastQC (version 0.11.5)