FastQCFastQC Report
Thu 26 May 2016
SRR1545693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545693_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences555290
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.2463577590088062No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.20871976804912748No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7810.14064722937564156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA302.163466E-645.00000415
GCGTTAA302.163466E-645.0000041
CGTAGGA453.8380676E-1045.0000044
CCCGCTA302.163466E-645.00000424
CGACGGT800.045.028
CTATCGG253.888192E-545.019
GCTATCG253.888192E-545.018
CTACGAT207.030135E-445.014
TCGTATC207.030135E-445.010
TCACTAC207.030135E-445.045
GTAAGGC950.045.04
CGCCGAC207.030135E-445.013
AGAATCG253.888192E-545.013
TGCGTAA207.030135E-445.01
TACCGCC207.030135E-445.034
CGTTTTA9700.043.376291
TAACGGG1000.042.753
CGGTCTA850.042.3529431
CGTTTTT13050.041.034481
AGGCGAT3900.040.9615367
Produced by FastQC (version 0.11.5)