##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545693_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	555290
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99325397540024	31.0	31.0	33.0	30.0	33.0
2	31.147605755551155	31.0	31.0	33.0	30.0	34.0
3	31.084391939347007	31.0	31.0	34.0	28.0	34.0
4	35.06486160384664	35.0	35.0	37.0	32.0	37.0
5	32.782207495182696	35.0	35.0	37.0	28.0	37.0
6	33.96404221217742	35.0	35.0	37.0	28.0	37.0
7	15.485490464442003	0.0	0.0	35.0	0.0	37.0
8	25.003522483747233	17.0	17.0	35.0	17.0	37.0
9	32.71476705865404	32.0	32.0	37.0	27.0	39.0
10	35.47990419420483	37.0	34.0	37.0	32.0	39.0
11	36.40604368888329	37.0	35.0	39.0	32.0	39.0
12	36.2774658286661	37.0	35.0	39.0	32.0	39.0
13	35.98622341479227	37.0	35.0	39.0	31.0	39.0
14	37.056851374957226	39.0	35.0	40.0	31.0	41.0
15	37.39426605917629	39.0	36.0	41.0	32.0	41.0
16	37.4235876749086	39.0	35.0	41.0	32.0	41.0
17	37.379236074843774	39.0	35.0	41.0	32.0	41.0
18	37.3128293324209	39.0	36.0	40.0	32.0	41.0
19	37.23998451259702	39.0	36.0	40.0	32.0	41.0
20	37.34462713176899	39.0	35.0	40.0	32.0	41.0
21	37.182756757730196	39.0	35.0	40.0	32.0	41.0
22	37.2462154910047	39.0	35.0	40.0	32.0	41.0
23	37.23024005474617	39.0	35.0	40.0	32.0	41.0
24	37.06902519404275	39.0	35.0	40.0	32.0	41.0
25	36.80726107079184	38.0	35.0	40.0	31.0	41.0
26	36.70237893713195	38.0	35.0	40.0	31.0	41.0
27	36.61930882962056	38.0	35.0	40.0	31.0	41.0
28	36.51708836824002	38.0	35.0	40.0	31.0	41.0
29	36.52418736155882	38.0	35.0	40.0	31.0	41.0
30	36.273934340614815	38.0	35.0	40.0	30.0	41.0
31	36.12178501323633	38.0	35.0	40.0	30.0	41.0
32	36.00583839075078	38.0	35.0	40.0	30.0	41.0
33	35.82113490248339	38.0	35.0	40.0	29.0	41.0
34	35.66140575194943	38.0	35.0	40.0	28.0	41.0
35	35.49307028759747	38.0	34.0	40.0	27.0	41.0
36	35.30678024095518	38.0	34.0	40.0	26.0	41.0
37	35.15275621747195	38.0	34.0	40.0	26.0	41.0
38	35.046599074357545	38.0	34.0	40.0	25.0	41.0
39	34.944709971366315	38.0	34.0	40.0	25.0	41.0
40	34.79231752777828	38.0	34.0	40.0	24.0	41.0
41	34.656033784148825	38.0	34.0	40.0	24.0	41.0
42	34.6121702173639	38.0	33.0	40.0	23.0	41.0
43	34.523544454249134	38.0	33.0	40.0	23.0	41.0
44	34.203427038124225	37.0	33.0	40.0	23.0	41.0
45	34.15437339048065	37.0	33.0	40.0	23.0	41.0
46	34.16082407390733	37.0	33.0	40.0	23.0	41.0
47	34.10504421113292	37.0	33.0	40.0	23.0	41.0
48	34.08374182859407	37.0	33.0	40.0	23.0	41.0
49	33.99171784112806	37.0	33.0	40.0	23.0	41.0
50	33.8716706585748	36.0	33.0	40.0	23.0	41.0
51	33.67485458048947	36.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	4.0
13	2.0
14	8.0
15	21.0
16	67.0
17	175.0
18	385.0
19	690.0
20	1138.0
21	1992.0
22	2913.0
23	4404.0
24	6228.0
25	8152.0
26	9837.0
27	10251.0
28	11175.0
29	13065.0
30	16344.0
31	20401.0
32	27233.0
33	40291.0
34	51722.0
35	55995.0
36	65483.0
37	99614.0
38	89430.0
39	18269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.6346413585694	24.15872787192278	28.946136253128994	15.260494516378827
2	32.78539141709737	24.114786868122962	30.04988384447766	13.049937870302003
3	30.425903581912156	24.146482018404797	32.77170487493022	12.65590952475283
4	27.0197554431018	28.985755190981287	29.102090799402113	14.892398566514794
5	20.81687046408183	37.05991463919754	28.84060580957698	13.282609087143655
6	19.780655153163213	41.77564876010733	28.92866790325776	9.5150281834717
7	38.773073529146934	56.28878603972699	4.208971888562733	0.7291685425633453
8	88.35635433737326	3.184282086837508	6.899638026976895	1.5597255488123323
9	84.02024167552091	5.212051360550343	8.155918529056889	2.6117884348718685
10	48.54994687460606	26.908282158871945	13.933800356570442	10.607970609951558
11	41.877757568117566	21.548560211781233	22.83041293738407	13.743269282717138
12	37.76657242161753	21.981847323020403	25.504150984170433	14.747429271191628
13	21.767184714293432	37.58468547965927	25.91024509715644	14.73788470889085
14	16.423670514505933	39.29928505825785	28.955140557186336	15.321903870049884
15	15.544130094185022	23.820706297610258	44.533667092870395	16.10149651533433
16	16.80131102667075	20.179905995065642	43.09748059572476	19.92130238253885
17	17.321759801184967	19.744818023015	30.39348808730573	32.5399340884943
18	21.512542995551872	23.276846332546956	34.37014893118911	20.840461740712062
19	28.80116695780583	24.526643735705665	26.140935367105477	20.531253939383028
20	29.9634425255272	24.078049307569017	25.9350969763547	20.02341119054908
21	22.621152911091503	28.110356750526748	28.88400655513335	20.384483783248392
22	22.27970970123719	23.922094761295902	26.693259377982674	27.104936159484232
23	19.78965945722055	29.711502098002846	26.76079165841272	23.73804678636388
24	19.76948981613211	24.1353166813737	37.9061391345063	18.189054367987897
25	18.490158295665328	25.43877973671415	35.00171081777089	21.069351149849627
26	16.906661384141618	33.384717895153884	28.21606728015992	21.49255344054458
27	18.738136829404457	31.67029840263646	29.444254353581012	20.147310414378072
28	16.400439410037997	27.91532352464478	37.774496209187994	17.90974085612923
29	16.59655315240685	25.436078445496946	36.83714815681896	21.130220245277243
30	19.717084766518393	29.80550703236147	31.24043292693908	19.23697527418106
31	28.822777287543445	26.12130598426048	25.63561382340759	19.42030290478849
32	28.364458211024868	26.948261268886526	26.650218804588594	18.03706171550001
33	26.902879576437538	26.917646635091575	25.343694285868644	20.835779502602243
34	19.40607610437789	28.13466837148157	28.120801743233265	24.338453780907273
35	20.31226926470853	25.50613193106305	31.067910461200455	23.113688343027967
36	28.963964775162527	26.321021448252264	25.51945830106791	19.1955554755173
37	20.230150011705593	32.41297340128582	27.711105908624322	19.645770678384267
38	20.75005852797637	32.91793477282141	24.503232545156585	21.828774154045632
39	21.347043886977975	30.805705127050732	25.797331124277406	22.04991986169389
40	23.798375623548058	25.866304093356625	26.639593725800932	23.695726557294385
41	17.999063552378036	25.589331700552865	27.308973689423542	29.102631057645556
42	21.92890201516325	26.434115507212447	24.658106574942824	26.97887590268148
43	22.038934610743937	25.724396261412956	26.11662374615066	26.120045381692446
44	20.237893713194907	29.214824686200004	28.909938950818493	21.637342649786596
45	18.479713302958814	35.19476309676025	24.370329017270258	21.95519458301068
46	21.806263393902288	31.785553494570408	26.33362747393254	20.07455563759477
47	21.569810369356553	27.59368978371662	27.212267463847716	23.62423238307911
48	22.488069297124024	25.653802517603413	30.22996992562445	21.62815825964811
49	20.86621405031605	25.279223468818095	31.48066775918889	22.37389472167696
50	19.818653316285186	32.42197770534315	26.725854958670244	21.033514019701418
51	19.67584505393578	32.10484611644366	25.533865187559652	22.685443642060903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	414.0
1	577.0
2	740.0
3	2883.0
4	5026.0
5	3919.0
6	2812.0
7	2942.0
8	3072.0
9	3112.0
10	3152.0
11	3214.0
12	3276.0
13	3156.5
14	3037.0
15	2966.5
16	2896.0
17	2782.5
18	2669.0
19	2636.5
20	2604.0
21	2668.5
22	2733.0
23	2922.0
24	3111.0
25	3430.0
26	4528.0
27	5307.0
28	6511.0
29	7715.0
30	8845.5
31	9976.0
32	11268.5
33	12561.0
34	14094.5
35	15628.0
36	17446.5
37	19265.0
38	25231.5
39	31198.0
40	37891.0
41	44584.0
42	48478.0
43	52372.0
44	51808.5
45	51245.0
46	48906.0
47	46567.0
48	43906.5
49	41246.0
50	39241.5
51	37237.0
52	33831.5
53	30426.0
54	27802.5
55	25179.0
56	23152.0
57	21125.0
58	19168.5
59	17212.0
60	15505.5
61	13799.0
62	12043.0
63	10287.0
64	8629.5
65	6972.0
66	6010.5
67	5049.0
68	4403.5
69	3758.0
70	3330.5
71	2903.0
72	2431.5
73	1960.0
74	1560.0
75	916.5
76	673.0
77	496.0
78	319.0
79	234.5
80	150.0
81	104.5
82	59.0
83	49.0
84	39.0
85	29.0
86	19.0
87	13.0
88	7.0
89	3.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	555290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.67059135876785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.5878579618631	22.03888613042146
2	7.99298525032864	5.062851391996342
3	4.205467909740356	3.995689669253952
4	2.762770939436744	3.4999435776312113
5	2.108836473757965	3.339404910142673
6	1.6575839066958111	3.149799753109981
7	1.3280372114822727	2.9441806683864837
8	1.148511363235625	2.9099227244749493
9	1.0095937333632838	2.8776987510949255
>10	8.065531826292647	46.41060651645576
>50	0.1129566723799483	2.335739604331895
>100	0.018163887015870586	0.8406001993269993
>500	5.676214692459558E-4	0.14039964834781574
>1k	0.0011352429384919116	0.45427645502551905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1368	0.2463577590088062	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1159	0.20871976804912748	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	781	0.14064722937564156	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009184390138486197	0.0
2	0.0	0.0	0.0	0.03151506420068793	0.0
3	0.0	0.0	0.0	0.04069945433917412	0.0
4	0.0	0.0	0.0	0.06230978407678871	0.0
5	0.0	0.0	0.0	0.10138846368564174	0.0
6	0.0	0.0	0.0	0.15811557924688	0.0
7	0.0	0.0	0.0	0.19809468926146698	0.0
8	0.0	0.0	0.0	0.47542725422752075	0.0
9	0.0	0.0	0.0	0.6225575825244467	0.0
10	0.0	0.0	0.0	0.8527075942300419	0.0
11	0.0	0.0	0.0	1.0203677357777017	0.0
12	0.0	0.0	0.0	1.1822651227286642	0.0
13	0.0	0.0	0.0	1.2494372309964163	0.0
14	0.0	0.0	0.0	1.2703272164094437	0.0
15	0.0	0.0	0.0	1.303282969259306	0.0
16	0.0	0.0	0.0	1.3783788650975166	0.0
17	0.0	0.0	0.0	1.4790469844585712	0.0
18	0.0	0.0	0.0	1.633020583839075	0.0
19	0.0	0.0	0.0	1.6983918312953592	0.0
20	0.0	0.0	0.0	1.775108501863891	0.0
21	0.0	0.0	0.0	1.855787066217652	0.0
22	0.0	0.0	0.0	1.9406076104377892	0.0
23	0.0	0.0	0.0	2.044517279259486	0.0
24	0.0	0.0	0.0	2.121954294152605	0.0
25	0.0	0.0	0.0	2.190567091069531	0.0
26	0.0	0.0	0.0	2.2535972194709073	0.0
27	0.0	0.0	0.0	2.3261719101730627	0.0
28	0.0	0.0	0.0	2.398026256550631	0.0
29	0.0	0.0	0.0	2.4774442183363647	0.0
30	0.0	0.0	0.0	2.5770318212105385	0.0
31	0.0	0.0	0.0	2.666534603540492	0.0
32	0.0	0.0	0.0	2.742530929784437	0.0
33	0.0	0.0	0.0	2.822309063732464	0.0
34	0.0	0.0	0.0	2.9078499522771883	0.0
35	0.0	0.0	0.0	3.0232851302922796	0.0
36	0.0	0.0	0.0	3.1217922166795726	0.0
37	0.0	0.0	0.0	3.2192187865799853	0.0
38	0.0	0.0	0.0	3.3107025158025536	0.0
39	0.0	0.0	0.0	3.4102901186767274	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGA	30	2.163466E-6	45.000004	15
GCGTTAA	30	2.163466E-6	45.000004	1
CGTAGGA	45	3.8380676E-10	45.000004	4
CCCGCTA	30	2.163466E-6	45.000004	24
CGACGGT	80	0.0	45.0	28
CTATCGG	25	3.888192E-5	45.0	19
GCTATCG	25	3.888192E-5	45.0	18
CTACGAT	20	7.030135E-4	45.0	14
TCGTATC	20	7.030135E-4	45.0	10
TCACTAC	20	7.030135E-4	45.0	45
GTAAGGC	95	0.0	45.0	4
CGCCGAC	20	7.030135E-4	45.0	13
AGAATCG	25	3.888192E-5	45.0	13
TGCGTAA	20	7.030135E-4	45.0	1
TACCGCC	20	7.030135E-4	45.0	34
CGTTTTA	970	0.0	43.37629	1
TAACGGG	100	0.0	42.75	3
CGGTCTA	85	0.0	42.35294	31
CGTTTTT	1305	0.0	41.03448	1
AGGCGAT	390	0.0	40.961536	7
>>END_MODULE