FastQCFastQC Report
Thu 26 May 2016
SRR1545692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545692_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences942419
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47170.5005204691331563No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.2199658538293477No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.1941811444803214No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13250.14059563739695402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG600.044.00000421
GGCCGTA207.855758E-444.00000435
ACAATCG207.855758E-444.0000045
CTCGACA207.855758E-444.00000431
TGCGTAA207.855758E-444.0000041
CACGACG1000.044.026
CGATATT254.4422282E-544.010
CGTTTTA27650.042.806511
CGTTATT15850.042.750791
CGGTCTA1000.041.831
GTACGGG2850.041.684213
AGGCGAT8600.041.1860477
GTTTTAT31200.040.967952
ACGGGGC1300.040.6153835
CGACGGT1050.039.8095228
TAGGACA1400.039.285716
GTTATTT18000.039.111112
CGTTTTT14150.038.869261
AAGGCGA4550.038.1978036
CTACGGG1100.038.03
Produced by FastQC (version 0.11.5)