FastQCFastQC Report
Thu 26 May 2016
SRR1545691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545691_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences361632
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12110.3348708078931068No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.31468454119104505No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5180.1432395363242191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG302.1613596E-645.0000045
CTCACGA600.045.00000424
CGGTCTA600.045.00000431
TTCATCG302.1613596E-645.00000415
TCGCCGT207.026867E-445.025
TATCACC207.026867E-445.044
CAACCCG253.8854858E-545.023
CGAATAT207.026867E-445.014
CGTTTTA7750.044.1290321
AGGCGAT2750.043.3636367
CGTTTTT9600.042.6562541
TAAGGCG750.042.05
AAGGCGA2000.041.6256
CGACGGT650.041.5384628
TACGGGC556.002665E-1140.9090924
CGTTATT2000.040.51
GTTTTAT8950.040.474862
GCGCGAC1900.040.2631579
AGGTACC900.040.07
GGTACCT1750.039.8571438