##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545691_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	361632
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.933459981417574	31.0	31.0	33.0	30.0	33.0
2	31.105709671710468	31.0	31.0	33.0	30.0	34.0
3	31.016530616759578	31.0	31.0	34.0	28.0	34.0
4	35.011948610742415	35.0	35.0	37.0	32.0	37.0
5	32.73493219626582	35.0	35.0	37.0	28.0	37.0
6	33.92207548004601	35.0	35.0	37.0	28.0	37.0
7	15.648615166799399	0.0	0.0	35.0	0.0	37.0
8	25.068442505088047	17.0	17.0	35.0	17.0	37.0
9	32.68442228563844	32.0	32.0	37.0	27.0	39.0
10	35.41533658525794	37.0	34.0	37.0	32.0	39.0
11	36.37857269268206	37.0	35.0	39.0	32.0	39.0
12	36.2949158260331	37.0	35.0	39.0	32.0	39.0
13	36.06816598088665	37.0	35.0	39.0	31.0	39.0
14	37.110839195646406	39.0	36.0	40.0	32.0	41.0
15	37.41874612866118	39.0	36.0	41.0	32.0	41.0
16	37.4264639191222	39.0	36.0	41.0	32.0	41.0
17	37.39246250331829	39.0	35.0	41.0	32.0	41.0
18	37.29799907087868	39.0	36.0	40.0	32.0	41.0
19	37.24419299177064	39.0	36.0	40.0	32.0	41.0
20	37.336499535439344	39.0	35.0	40.0	32.0	41.0
21	37.17399455800371	39.0	35.0	40.0	32.0	41.0
22	37.22819883196178	39.0	35.0	40.0	32.0	41.0
23	37.19320192903283	39.0	35.0	40.0	32.0	41.0
24	37.000478386868416	39.0	35.0	40.0	32.0	41.0
25	36.74723475798602	38.0	35.0	40.0	31.0	41.0
26	36.61230477391381	38.0	35.0	40.0	31.0	41.0
27	36.52915118131139	38.0	35.0	40.0	31.0	41.0
28	36.43214372621892	38.0	35.0	40.0	31.0	41.0
29	36.42370420759225	38.0	35.0	40.0	30.0	41.0
30	36.13854415538448	38.0	35.0	40.0	30.0	41.0
31	36.005041036191486	38.0	35.0	40.0	30.0	41.0
32	35.87273250154853	38.0	34.0	40.0	29.0	41.0
33	35.67009280152199	38.0	34.0	40.0	28.0	41.0
34	35.47323522254668	38.0	34.0	40.0	27.0	41.0
35	35.271043491726395	38.0	34.0	40.0	26.0	41.0
36	35.060608574462435	38.0	34.0	40.0	25.0	41.0
37	34.92440381382178	38.0	34.0	40.0	24.0	41.0
38	34.79988773117423	38.0	34.0	40.0	24.0	41.0
39	34.68895451729935	38.0	33.0	40.0	23.0	41.0
40	34.5365703256349	38.0	33.0	40.0	23.0	41.0
41	34.37326619325724	38.0	33.0	40.0	23.0	41.0
42	34.319628793912045	37.0	33.0	40.0	23.0	41.0
43	34.2453516281745	37.0	33.0	40.0	23.0	41.0
44	34.04044996018052	37.0	33.0	40.0	22.0	41.0
45	33.956759910627376	37.0	33.0	40.0	22.0	41.0
46	33.92453101495443	37.0	33.0	40.0	22.0	41.0
47	33.829390098221396	37.0	33.0	40.0	22.0	41.0
48	33.76937605079196	36.0	33.0	40.0	22.0	41.0
49	33.65923922661711	36.0	33.0	40.0	21.0	41.0
50	33.53398758959384	36.0	32.0	40.0	20.0	41.0
51	33.33612899300947	36.0	32.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	4.0
14	7.0
15	23.0
16	40.0
17	138.0
18	267.0
19	531.0
20	872.0
21	1455.0
22	2137.0
23	3058.0
24	4388.0
25	5718.0
26	7033.0
27	7173.0
28	7992.0
29	8798.0
30	10647.0
31	13668.0
32	17771.0
33	25359.0
34	32386.0
35	36167.0
36	43605.0
37	64869.0
38	55543.0
39	11981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.332050261038844	22.93823555437572	26.58863153703212	15.141082647553313
2	35.769235023449255	22.372190514113797	29.27257543580214	12.585999026634811
3	30.651878152375893	23.507322360853024	32.75871604282807	13.082083443943013
4	27.435901690115923	26.54466418900982	30.347978055039377	15.671456065834882
5	21.018881072471462	35.977734271303426	28.852811698079815	14.150572958145297
6	19.480576940093798	40.97839792938678	29.67878948765596	9.862235642863464
7	38.13213432439607	55.863419166445446	5.13726661357402	0.8671798955844615
8	85.58534642951952	3.6385054419962835	8.79374612866118	1.9824019998230247
9	80.99089682328997	5.112932483850987	10.515109282364392	3.3810614104946466
10	45.43900982213963	25.114204495177418	16.82815680028316	12.618628882399788
11	39.78823776656933	23.112722325457923	22.543082470577826	14.55595743739492
12	35.14401380408813	22.386293248385098	27.420692859039026	15.049000088487743
13	23.05492876736572	33.940580479603575	27.666799398283338	15.337691354747369
14	18.254468631094596	36.79984072206	29.409454915494205	15.536235731351208
15	16.44848907176356	23.830302628086013	43.30009512432528	16.42111317582515
16	18.62584063357225	19.873794354481905	42.15003097071055	19.35033404123529
17	19.553579329262895	19.712027696664013	30.18842359083267	30.545969383240422
18	22.236417131227327	22.47063312981152	34.6241483054597	20.66880143350146
19	28.728375807450668	24.26002123705867	27.015584903990796	19.996018051499867
20	29.949783205026105	23.78080479603575	26.069319086806473	20.20009291213167
21	24.337448013450135	27.005630032740463	29.27285196000354	19.38406999380586
22	23.554054950889302	23.607147597557738	27.539045217237412	25.299752234315548
23	20.76945624281037	29.256260507919652	26.900827360410585	23.073455888859392
24	20.836098575347314	25.023228032917437	36.206143261658255	17.934530130076983
25	20.870664100522077	23.933722679408902	34.16705380054862	21.028559419520395
26	18.70769179718609	31.02712149367313	28.529554906645433	21.73563180249535
27	19.42029466418901	30.02002035218122	30.295991505176534	20.263693478453234
28	18.065602601539688	26.776114945580037	36.444230599062024	18.714051853818244
29	18.635518980621185	24.911788779754005	36.36818644367755	20.084505795947262
30	20.926798513405892	27.689750906999382	31.78728652331652	19.596164056278205
31	28.41894522608619	24.679508450579597	27.070336695867624	19.831209627466595
32	28.34981417573666	25.86911556499425	27.447792230776034	18.33327802849305
33	27.041301654720822	24.965710999026637	27.439496504734095	20.55349084151845
34	20.80319219538094	26.87455756127776	28.44991593664277	23.872334306698523
35	21.236505618971773	25.028758516945405	31.415361472436064	22.319374391646758
36	28.794465091584815	25.17255110167242	26.481340146889654	19.55164365985311
37	21.364812848420495	30.757233873108575	27.518305902132557	20.359647376338376
38	22.06967303778427	32.088974427041855	24.53405672064419	21.307295814529688
39	21.830203079373504	29.065458808954958	25.99078621360941	23.113551898062116
40	24.40602601539687	25.17669896469339	26.668547031236173	23.74872798867357
41	20.06127776302982	24.17512830722945	28.108685072117513	27.654908857623216
42	22.862744447394036	26.484381913105036	25.2635275639324	25.389346075568536
43	22.514047429431024	26.788005486240156	25.745232722767895	24.952714361560922
44	21.14193434209362	28.374148305459695	28.621913989912397	21.86200336253429
45	19.90172329882311	32.58561189275285	25.480875586231306	22.031789222192728
46	22.322139633660736	29.804331475090702	27.445580037164852	20.42794885408371
47	21.995841076010972	27.645783558977083	26.79768383328909	23.560691531722856
48	22.55524953543934	25.92552650207946	29.934574373949207	21.58464958853199
49	21.41928811609592	25.241405627820544	31.203820458366515	22.135485797717017
50	20.70087824086364	30.43535970268118	27.286302097159542	21.577459959295638
51	20.318998318732856	31.65649057605522	26.043602336076454	21.980908769135475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	207.0
1	358.5
2	510.0
3	2566.5
4	4623.0
5	3438.5
6	2254.0
7	2222.5
8	2191.0
9	2253.0
10	2315.0
11	2293.5
12	2272.0
13	2142.5
14	2013.0
15	1973.0
16	1933.0
17	1841.5
18	1750.0
19	1764.0
20	1778.0
21	1802.5
22	1827.0
23	1990.5
24	2154.0
25	2286.0
26	2854.5
27	3291.0
28	3880.5
29	4470.0
30	5167.0
31	5864.0
32	6891.5
33	7919.0
34	8729.0
35	9539.0
36	10798.5
37	12058.0
38	15061.5
39	18065.0
40	21883.5
41	25702.0
42	27677.0
43	29652.0
44	29456.5
45	29261.0
46	29208.0
47	29155.0
48	28295.5
49	27436.0
50	26184.5
51	24933.0
52	22829.5
53	20726.0
54	19242.5
55	17759.0
56	16764.5
57	15770.0
58	14527.5
59	13285.0
60	11990.0
61	10695.0
62	9461.5
63	8228.0
64	7216.5
65	6205.0
66	5289.0
67	4373.0
68	3787.5
69	3202.0
70	2733.5
71	2265.0
72	1913.5
73	1562.0
74	1241.0
75	721.0
76	522.0
77	390.0
78	258.0
79	196.5
80	135.0
81	102.5
82	70.0
83	56.0
84	42.0
85	26.5
86	11.0
87	10.0
88	9.0
89	5.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	361632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.049173835876374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.47680693833102	30.163467725522885
2	10.432460808604382	9.190825593883636
3	5.7548729629963455	7.60492198651233
4	3.6287942628592043	6.393815571972638
5	2.6836975686129567	5.910733036137546
6	1.9557154770890466	5.1688590614285586
7	1.47558167634068	4.549870763911514
8	1.1871365426366265	4.183390714681763
9	0.9169830547375422	3.635311138441824
>10	3.461215050142475	21.467061709477107
>50	0.018031024926565706	0.5617277042005243
>100	0.0068393542824904405	0.3848012795717878
>500	6.217594802264036E-4	0.14186979560013244
>1k	0.0012435189604528072	0.6433439186577434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1211	0.3348708078931068	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.31468454119104505	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	518	0.1432395363242191	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0074661534377488716	0.0
2	0.0	0.0	0.0	0.03567162198035572	0.0
3	0.0	0.0	0.0	0.04452039642509512	0.0
4	0.0	0.0	0.0	0.06415361472436068	0.0
5	0.0	0.0	0.0	0.10950358375365012	0.0
6	0.0	0.0	0.0	0.17586939208919564	0.0
7	0.0	0.0	0.0	0.22011326431289266	0.0
8	0.0	0.0	0.0	0.5076984337669233	0.0
9	0.0	0.0	0.0	0.6476196796743651	0.0
10	0.0	0.0	0.0	0.8743695248208123	0.0
11	0.0	0.0	0.0	1.0264578355897709	0.0
12	0.0	0.0	0.0	1.1708034687195823	0.0
13	0.0	0.0	0.0	1.2316387930271657	0.0
14	0.0	0.0	0.0	1.2620564551809574	0.0
15	0.0	0.0	0.0	1.299940270772498	0.0
16	0.0	0.0	0.0	1.3770905229625696	0.0
17	0.0	0.0	0.0	1.466684364215556	0.0
18	0.0	0.0	0.0	1.6154543845677374	0.0
19	0.0	0.0	0.0	1.6704827006459606	0.0
20	0.0	0.0	0.0	1.729105831342359	0.0
21	0.0	0.0	0.0	1.8109569949561986	0.0
22	0.0	0.0	0.0	1.8881072471462703	0.0
23	0.0	0.0	0.0	1.9865498628439962	0.0
24	0.0	0.0	0.0	2.0601053004158922	0.0
25	0.0	0.0	0.0	2.126471108751438	0.0
26	0.0	0.0	0.0	2.1884125298646135	0.0
27	0.0	0.0	0.0	2.2498009025749934	0.0
28	0.0	0.0	0.0	2.321697194938501	0.0
29	0.0	0.0	0.0	2.4013361649411555	0.0
30	0.0	0.0	0.0	2.50143792584727	0.0
31	0.0	0.0	0.0	2.59490310591983	0.0
32	0.0	0.0	0.0	2.680349084151845	0.0
33	0.0	0.0	0.0	2.769666401203433	0.0
34	0.0	0.0	0.0	2.8578776214494295	0.0
35	0.0	0.0	0.0	2.963786390584904	0.0
36	0.0	0.0	0.0	3.0536567560392887	0.0
37	0.0	0.0	0.0	3.1316365808335545	0.0
38	0.0	0.0	0.0	3.2198478010795504	0.0
39	0.0	0.0	0.0	3.3417949738961155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	30	2.1613596E-6	45.000004	5
CTCACGA	60	0.0	45.000004	24
CGGTCTA	60	0.0	45.000004	31
TTCATCG	30	2.1613596E-6	45.000004	15
TCGCCGT	20	7.026867E-4	45.0	25
TATCACC	20	7.026867E-4	45.0	44
CAACCCG	25	3.8854858E-5	45.0	23
CGAATAT	20	7.026867E-4	45.0	14
CGTTTTA	775	0.0	44.129032	1
AGGCGAT	275	0.0	43.363636	7
CGTTTTT	960	0.0	42.656254	1
TAAGGCG	75	0.0	42.0	5
AAGGCGA	200	0.0	41.625	6
CGACGGT	65	0.0	41.53846	28
TACGGGC	55	6.002665E-11	40.909092	4
CGTTATT	200	0.0	40.5	1
GTTTTAT	895	0.0	40.47486	2
GCGCGAC	190	0.0	40.263157	9
AGGTACC	90	0.0	40.0	7
GGTACCT	175	0.0	39.857143	8
>>END_MODULE