FastQCFastQC Report
Thu 26 May 2016
SRR1545690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545690_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1194783
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58160.4867829555659898No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24750.20715058717775528No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24510.20514185421118314No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA16010.133999228311752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG600.044.02
CCCGTTA207.8566076E-444.031
CGTTTTA33800.042.7633131
CGTTATT19100.042.5026131
TTGCGAA1150.042.086961
AGGCGAT8850.041.0169537
GTTATTT21700.040.654382
TCGTAGA603.6379788E-1240.3333321
GTTTTAT39100.039.948852
CGTTTTT18800.039.3191451
GTACGGG2750.039.23
GCGATAT850.038.823539
TTACGAA404.1247222E-738.51
TTACGGG3500.038.3428573
GGTACCT9850.038.192898
GTTGATC9450.038.17989316
GTAGCAT9400.037.9148929
ACGTTAG357.2904495E-637.7142872
GCGATCG357.2904495E-637.7142879
TACGGGG6300.037.7142834
Produced by FastQC (version 0.11.5)