##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545690_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1194783
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.505164536154265	31.0	31.0	33.0	30.0	34.0
2	31.695958178179637	31.0	31.0	34.0	30.0	34.0
3	31.715257917128046	31.0	31.0	34.0	30.0	34.0
4	35.638221333915865	37.0	35.0	37.0	33.0	37.0
5	27.23153995328022	35.0	26.0	37.0	0.0	37.0
6	31.3945938300093	35.0	28.0	37.0	17.0	37.0
7	14.901777979767036	0.0	0.0	32.0	0.0	37.0
8	25.039876697274735	17.0	17.0	35.0	17.0	37.0
9	33.14231370884922	32.0	32.0	37.0	28.0	39.0
10	35.99884916340457	37.0	35.0	39.0	32.0	39.0
11	36.98782707822257	39.0	37.0	39.0	34.0	39.0
12	37.00494985281846	39.0	35.0	39.0	33.0	39.0
13	36.5562273651366	39.0	35.0	39.0	33.0	39.0
14	37.86919298316096	40.0	36.0	41.0	33.0	41.0
15	38.130324083954996	40.0	37.0	41.0	33.0	41.0
16	38.19375233829072	40.0	36.0	41.0	33.0	41.0
17	38.22508103982062	40.0	36.0	41.0	34.0	41.0
18	38.18699797369062	40.0	36.0	41.0	34.0	41.0
19	38.11364406758382	40.0	36.0	41.0	34.0	41.0
20	38.1289313624315	40.0	36.0	41.0	34.0	41.0
21	37.990710447001675	40.0	36.0	41.0	34.0	41.0
22	37.88398562751562	40.0	35.0	41.0	33.0	41.0
23	37.76950040300205	40.0	35.0	41.0	33.0	41.0
24	37.60065049469234	39.0	35.0	41.0	33.0	41.0
25	37.41824247583034	39.0	35.0	41.0	33.0	41.0
26	37.179404126104906	39.0	35.0	40.0	33.0	41.0
27	36.95802919860761	39.0	35.0	40.0	32.0	41.0
28	36.961121810404066	39.0	35.0	40.0	32.0	41.0
29	36.881357535217695	39.0	35.0	40.0	32.0	41.0
30	36.657979733558314	38.0	35.0	40.0	31.0	41.0
31	36.44585669531622	38.0	35.0	40.0	31.0	41.0
32	36.18282315700843	38.0	35.0	40.0	30.0	41.0
33	35.929949622651144	38.0	35.0	40.0	30.0	41.0
34	35.65057336771615	38.0	35.0	40.0	29.0	41.0
35	35.419425954336475	38.0	35.0	40.0	27.0	41.0
36	35.21048508390226	38.0	34.0	40.0	26.0	41.0
37	35.057979566163894	38.0	34.0	40.0	26.0	41.0
38	34.86754331121216	38.0	34.0	40.0	25.0	41.0
39	34.78480025243078	38.0	34.0	40.0	24.0	41.0
40	34.52692664693087	37.0	34.0	40.0	24.0	41.0
41	34.33695574844972	37.0	34.0	40.0	23.0	40.0
42	34.31973421114964	37.0	33.0	40.0	23.0	40.0
43	34.19316227298179	37.0	33.0	40.0	23.0	40.0
44	33.954605982843745	37.0	33.0	40.0	23.0	40.0
45	33.731596448894905	36.0	33.0	39.0	23.0	40.0
46	33.676302726101724	36.0	33.0	39.0	23.0	40.0
47	33.500053147726405	36.0	33.0	39.0	23.0	40.0
48	33.413340330419835	36.0	33.0	39.0	22.0	40.0
49	33.278196961289204	35.0	33.0	39.0	22.0	40.0
50	33.33695491147765	35.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	6.0
14	13.0
15	25.0
16	78.0
17	188.0
18	462.0
19	1053.0
20	1911.0
21	3289.0
22	5389.0
23	8101.0
24	12150.0
25	17212.0
26	21700.0
27	22978.0
28	23332.0
29	25488.0
30	30046.0
31	39657.0
32	54180.0
33	96148.0
34	100555.0
35	125017.0
36	164926.0
37	287057.0
38	123077.0
39	30738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.98657580497881	22.468849992006916	27.26344449159387	17.2811297114204
2	36.09450419030066	23.331851892770487	28.379881534973293	12.193762381955551
3	30.652260703408068	23.105199856375595	33.45377361412073	12.788765826095617
4	26.79474013272703	26.49954008384786	31.26969499900819	15.436024784416919
5	17.373196639054957	46.972797570772265	23.997914265603043	11.656091524569733
6	19.781918557595816	39.99797452759204	29.99306150154463	10.227045413267515
7	37.89064625124395	56.13655366706758	4.893691992604515	1.079108089083959
8	84.79707193691239	3.2318002515938042	9.866896331802511	2.1042314796912915
9	80.18025030486707	4.8758644875261865	11.651488178188005	3.292397029418731
10	48.183645063580585	21.964574320190362	17.335867684759492	12.515912931469565
11	41.60889466957598	21.235404253324663	23.001666411390183	14.154034665709169
12	36.78425287269739	19.973417767075695	27.86564589553082	15.3766834646961
13	22.775935044271638	33.0942104131043	28.63214491669199	15.497709625932075
14	17.73820015852251	36.33203686359783	30.553246907597448	15.376516070282218
15	16.15866646914126	22.01763834939064	45.28546187885164	16.538233302616458
16	18.455234130381832	17.545529188145462	44.02933419708851	19.969902484384193
17	18.872966890221907	17.995987555899273	30.883683480598567	32.24736207328025
18	22.693995478676882	21.851331999199854	34.33242689258217	21.122245629541098
19	27.90983802079541	24.001345851087603	27.5391430912559	20.549673036861087
20	29.356460545555134	23.23292179416681	26.524649245930014	20.885968414348042
21	23.983518346009276	25.440268232808805	30.18857817695766	20.387635244224263
22	23.328252912872045	22.441899491372073	28.25316396366537	25.976683632090513
23	21.194560016337693	27.66443781004584	26.661326784863864	24.479675388752604
24	21.171794376049878	23.807921605848094	36.01867452081257	19.001609497289465
25	20.590182485020293	23.252004757349244	34.20060379165087	21.957208965979596
26	19.13117277363337	29.206224059096925	29.32507409295245	22.33752907431726
27	19.842766427041564	28.008098541743564	30.952817373531428	21.196317657683444
28	18.48134765894727	25.56681840970285	37.32786623177598	18.6239676995739
29	19.032577463857454	23.724224398907584	35.869944584079285	21.373253553155678
30	21.305626209947746	25.893321213977767	33.07236544209283	19.72868713398165
31	29.072308527992114	23.788922339872595	26.723681204034538	20.41508792810075
32	28.68428827661592	24.842837569667463	27.47461254470477	18.998261609011845
33	27.11471455486059	24.73955521630288	27.412760308775734	20.7329699200608
34	21.725535097168272	26.070842989898583	29.208316489270437	22.995305423662707
35	22.318780899962587	24.52729909950175	30.81588874297676	22.338031257558903
36	29.913716549365034	23.55013420847133	26.237567826124074	20.298581416039564
37	22.252660106479585	28.971620788042685	27.952356201921187	20.823362903556543
38	23.450534532212124	29.548880424311356	24.74181504089027	22.258770002586246
39	22.456881291414422	27.633553540684797	26.54883773873582	23.360727429164964
40	24.21008668519723	24.510308566492828	27.279179566498684	24.00042518181126
41	20.30594677025033	23.567878016342718	28.470023426848222	27.65615178655873
42	23.913798572627833	25.872648003863464	24.77813962870245	25.435413794806255
43	23.288329345161422	25.893739700012468	26.565242391296163	24.252688563529947
44	21.960222065429456	27.674648869292582	28.29769087775772	22.067438187520246
45	20.33197660160883	31.24023358216513	25.82117422159505	22.60661559463099
46	23.898984166999362	27.707792963241022	27.69415031850972	20.69907255124989
47	23.32749963800958	26.546243125320668	27.633804632305615	22.49245260436414
48	23.749417258196676	24.63568698248971	28.741369771749348	22.87352598756427
49	22.075640513800415	24.059599107118196	30.643137707851555	23.221622671229838
50	21.191044733646194	28.736933819781502	28.11949952418138	21.95252192239093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1331.0
1	1769.5
2	2208.0
3	10217.5
4	18227.0
5	13294.5
6	8362.0
7	8550.0
8	8738.0
9	8944.5
10	9151.0
11	9102.5
12	9054.0
13	8727.0
14	8400.0
15	7945.0
16	7490.0
17	6988.5
18	6487.0
19	6323.5
20	6160.0
21	6125.5
22	6091.0
23	6468.0
24	6845.0
25	7342.5
26	7840.0
27	9042.5
28	10245.0
29	11933.5
30	13622.0
31	15787.0
32	17952.0
33	19863.0
34	21774.0
35	23790.0
36	25806.0
37	32111.0
38	38416.0
39	50252.5
40	62089.0
41	73800.0
42	85511.0
43	91397.0
44	97283.0
45	98324.0
46	99365.0
47	99316.5
48	99268.0
49	94602.0
50	89936.0
51	81858.0
52	73780.0
53	68063.5
54	62347.0
55	60200.0
56	58053.0
57	56022.0
58	53991.0
59	50269.5
60	46548.0
61	42599.0
62	38650.0
63	33902.5
64	29155.0
65	25263.5
66	21372.0
67	18434.5
68	15497.0
69	13236.0
70	10975.0
71	9088.5
72	7202.0
73	5710.0
74	4218.0
75	3373.5
76	2529.0
77	1962.0
78	1395.0
79	1108.0
80	821.0
81	586.5
82	352.0
83	247.5
84	143.0
85	106.5
86	70.0
87	42.0
88	14.0
89	9.5
90	5.0
91	6.0
92	7.0
93	4.5
94	2.0
95	3.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1194783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.591634088483225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43972504391866	20.425784778375736
2	7.343690066272577	4.199361984681893
3	3.614469346729067	3.1003075495714945
4	2.3959642360559954	2.74018130905621
5	1.8076809259450575	2.584227579167581
6	1.3801656115267853	2.367671408776892
7	1.209853762989712	2.4214187264383447
8	1.0027257148915403	2.293565338503334
9	0.8655598938161633	2.227299458909232
>10	8.62126189704203	49.439390272819075
>50	0.27694262325592717	5.045213608298246
>100	0.04028244245427201	1.8710030186722162
>500	0.0	0.0
>1k	0.0013427480817689548	0.7263645657659074
>5k	3.356870204422387E-4	0.5582104009638633
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5816	0.4867829555659898	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2475	0.20715058717775528	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2451	0.20514185421118314	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1601	0.133999228311752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004017465933144345	0.0
2	0.0	0.0	0.0	0.026113528565438242	0.0
3	0.0	0.0	0.0	0.04385733643682577	0.0
4	0.0	0.0	0.0	0.06386096889560698	0.0
5	0.0	0.0	0.0	0.10763460812549225	0.0
6	0.0	0.0	0.0	0.23108798836274036	0.0
7	0.0	0.0	0.0	0.3175472031322843	0.0
8	0.0	0.0	0.0	0.8071758637342513	0.0
9	0.0	0.0	0.0	1.0840462242934492	0.0
10	0.0	0.0	0.0	1.4836166902274304	0.0
11	0.0	0.0	0.0	1.740650812741728	0.0
12	0.0	0.0	0.0	1.9755888726237316	0.0
13	0.0	0.0	0.0	2.083056086335343	0.0
14	0.0	0.0	0.0	2.1259090562888825	0.0
15	0.0	0.0	0.0	2.171189245243697	0.0
16	0.0	0.0	0.0	2.272965048883354	0.0
17	0.0	0.0	0.0	2.380599657008846	0.0
18	0.0	0.0	0.0	2.5496680150286704	0.0
19	0.0	0.0	0.0	2.622484585066912	0.0
20	0.0	0.0	0.0	2.70308499535062	0.0
21	0.0	0.0	0.0	2.801261819091835	0.0
22	0.0	0.0	0.0	2.89943864283305	0.0
23	0.0	0.0	0.0	3.0105885336500435	0.0
24	0.0	0.0	0.0	3.100563031111089	0.0
25	0.0	0.0	0.0	3.1671860078357326	0.0
26	0.0	0.0	0.0	3.2388308169768067	0.0
27	0.0	0.0	0.0	3.3158322473620734	0.0
28	0.0	0.0	0.0	3.3981902989915325	0.0
29	0.0	0.0	0.0	3.489169162935864	0.0
30	0.0	0.0	0.0	3.6135432124494575	0.0
31	0.0	0.0	0.0	3.714482044019709	0.0
32	0.0	0.0	0.0	3.8068000632750882	0.0
33	0.0	0.0	0.0	3.894682130562621	0.0
34	0.0	0.0	0.0	3.9892599744053943	0.0
35	0.0	0.0	0.0	4.111457896538535	0.0
36	0.0	0.0	0.0	4.2144054610753585	0.0
37	0.0	0.0	0.0	4.316934539577479	0.0
38	0.0	0.0	0.0	4.4352823901913565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	60	0.0	44.0	2
CCCGTTA	20	7.8566076E-4	44.0	31
CGTTTTA	3380	0.0	42.763313	1
CGTTATT	1910	0.0	42.502613	1
TTGCGAA	115	0.0	42.08696	1
AGGCGAT	885	0.0	41.016953	7
GTTATTT	2170	0.0	40.65438	2
TCGTAGA	60	3.6379788E-12	40.333332	1
GTTTTAT	3910	0.0	39.94885	2
CGTTTTT	1880	0.0	39.319145	1
GTACGGG	275	0.0	39.2	3
GCGATAT	85	0.0	38.82353	9
TTACGAA	40	4.1247222E-7	38.5	1
TTACGGG	350	0.0	38.342857	3
GGTACCT	985	0.0	38.19289	8
GTTGATC	945	0.0	38.179893	16
GTAGCAT	940	0.0	37.91489	29
ACGTTAG	35	7.2904495E-6	37.714287	2
GCGATCG	35	7.2904495E-6	37.714287	9
TACGGGG	630	0.0	37.714283	4
>>END_MODULE