FastQCFastQC Report
Thu 26 May 2016
SRR1545689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545689_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences449881
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.34786977000584596No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.32764219871477124No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5990.13314632091597556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT207.0287037E-445.033
CGGGATA253.887008E-545.06
ACGTAAG207.0287037E-445.02
CGTTTTA8950.043.994411
CGTTATT1900.042.631581
GCATAAG800.042.18752
CGGTCTA700.041.78571331
TCACGAC650.041.5384625
TAAGGCC650.041.538465
ACGACGG650.041.5384627
CGTTTTT15200.041.2993431
GTTTTAT10600.041.1792452
AGGCGAT3850.040.9090927
CGCAGTA950.040.26315737
GTTGATC3250.040.15384716
CGCATCG451.9237632E-840.00000421
CGCCAGT451.9237632E-840.00000426
GGTACCT3400.039.7058838
CAGTAGC3350.039.62686527
ATCCTGC3300.039.54545220