##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545689_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	449881
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.004396718243267	31.0	31.0	33.0	30.0	33.0
2	31.17457060867207	31.0	31.0	33.0	30.0	34.0
3	31.07714039935005	31.0	31.0	34.0	28.0	34.0
4	35.05212044963001	35.0	35.0	37.0	32.0	37.0
5	32.773095551934844	35.0	35.0	37.0	28.0	37.0
6	33.978083093084614	35.0	35.0	37.0	28.0	37.0
7	15.209886614460268	0.0	0.0	35.0	0.0	37.0
8	24.858384772862156	17.0	17.0	35.0	17.0	37.0
9	32.664071165485986	32.0	32.0	37.0	27.0	39.0
10	35.48484154698687	37.0	34.0	37.0	32.0	39.0
11	36.45825451619428	37.0	35.0	39.0	32.0	39.0
12	36.34225495186505	37.0	35.0	39.0	32.0	39.0
13	36.05621042008887	37.0	35.0	39.0	31.0	39.0
14	37.10158908689187	39.0	36.0	40.0	32.0	41.0
15	37.42065790731327	39.0	36.0	41.0	32.0	41.0
16	37.452959782698095	39.0	36.0	41.0	32.0	41.0
17	37.40939715169122	39.0	35.0	41.0	32.0	41.0
18	37.32045585388136	39.0	36.0	40.0	32.0	41.0
19	37.23737388331581	39.0	36.0	40.0	32.0	41.0
20	37.343995412120094	39.0	35.0	40.0	32.0	41.0
21	37.19476261500263	39.0	35.0	40.0	32.0	41.0
22	37.227431254042735	39.0	35.0	40.0	32.0	41.0
23	37.195502810743285	39.0	35.0	40.0	32.0	41.0
24	36.99865964555071	39.0	35.0	40.0	32.0	41.0
25	36.73684596593321	38.0	35.0	40.0	31.0	41.0
26	36.62572991524426	38.0	35.0	40.0	31.0	41.0
27	36.51667218664491	38.0	35.0	40.0	31.0	41.0
28	36.40285986738715	38.0	35.0	40.0	31.0	41.0
29	36.37127595964266	38.0	35.0	40.0	31.0	41.0
30	36.066175277462264	38.0	35.0	40.0	30.0	41.0
31	35.9179916466799	38.0	35.0	40.0	30.0	41.0
32	35.777850142593266	38.0	34.0	40.0	29.0	41.0
33	35.53012685576852	38.0	34.0	40.0	27.0	41.0
34	35.31881986569782	38.0	34.0	40.0	26.0	41.0
35	35.075677790349005	38.0	34.0	40.0	25.0	41.0
36	34.84452110669266	38.0	34.0	40.0	24.0	41.0
37	34.67526968242713	38.0	33.0	40.0	23.0	41.0
38	34.54458178940653	38.0	33.0	40.0	23.0	41.0
39	34.42703737210507	38.0	33.0	40.0	23.0	41.0
40	34.23905655051002	37.0	33.0	40.0	23.0	41.0
41	34.091939868543015	37.0	33.0	40.0	22.0	41.0
42	34.03031690602626	37.0	33.0	40.0	22.0	41.0
43	33.93797915448752	37.0	33.0	40.0	21.0	41.0
44	33.69738664224539	37.0	33.0	40.0	20.0	41.0
45	33.600092024335325	36.0	33.0	40.0	20.0	41.0
46	33.57478533212116	36.0	33.0	40.0	20.0	41.0
47	33.47279613942354	36.0	32.0	40.0	20.0	41.0
48	33.418070556436035	36.0	32.0	40.0	20.0	41.0
49	33.306483270020294	36.0	32.0	40.0	20.0	41.0
50	33.16218066555378	35.0	32.0	40.0	20.0	40.0
51	32.9809660776961	35.0	32.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	7.0
14	7.0
15	17.0
16	62.0
17	138.0
18	322.0
19	641.0
20	1225.0
21	1798.0
22	2849.0
23	4044.0
24	5698.0
25	7670.0
26	8992.0
27	9469.0
28	10000.0
29	11346.0
30	13297.0
31	17271.0
32	22441.0
33	33381.0
34	41918.0
35	45488.0
36	54164.0
37	78834.0
38	65836.0
39	12966.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.597953681084554	23.32216741760599	27.52261153505038	16.557267366259076
2	35.66454240121277	23.22480833820499	28.94832188956635	12.162327371015891
3	31.371184824431346	22.908724751656546	32.88869723326835	12.831393190643748
4	27.168517896954974	27.148734887670294	30.039721615271592	15.643025600103138
5	21.262511641967542	35.57518543792692	28.867411604402054	14.294891315703486
6	19.72410481882987	40.815015526328075	29.427115170456187	10.033764484385872
7	36.823737832893585	57.212907413293735	5.159142084240054	0.8042126695726203
8	85.60463767085074	3.433130094402742	9.00993818365301	1.9522940510935112
9	80.877832137832	5.054670012736701	10.709721015112885	3.3577768343184085
10	48.49638015386291	22.612424174392785	16.48080270115875	12.410392970585555
11	42.00355205043111	21.727523500659064	22.221654170769604	14.04727027814022
12	37.217175208555155	20.474525485628423	27.158515251811032	15.14978405400539
13	23.051651436713264	34.05811759109631	27.45726092010998	15.432970052080439
14	17.722019823019867	37.266521591265246	29.93058164270107	15.080876943013818
15	16.14471382432243	22.457049753156948	45.025240007913204	16.372996414607417
16	18.442210273383406	17.920961320882633	43.59486175233006	20.0419666534039
17	18.749180338800706	18.44621133144098	30.16553266308202	32.6390756666763
18	22.80580864717559	22.0931757509208	34.16614615865085	20.934869443252772
19	28.648242535248208	24.05525016615505	26.837319202189025	20.459188096407715
20	29.951031494995345	23.438420382278867	25.85772682109269	20.752821301633098
21	24.15505433659123	26.021770201453275	29.556260433314584	20.26691502864091
22	23.416636843965406	22.728010296056066	27.463929350205945	26.391423509772583
23	21.137367437166716	28.188343139630256	26.08667625438727	24.587613168815754
24	21.08024121934467	24.092815655695617	35.93839259715347	18.88855052780624
25	20.525205554357708	23.54978316488138	34.082568501448165	21.84244277931275
26	19.06015146227558	29.947474998944166	28.63735076609148	22.35502277268878
27	19.725216223845862	29.102140343779798	30.261113494457426	20.911529937916914
28	18.45599169558172	26.151804588324467	36.97333294804626	18.41887076804755
29	18.9112231901325	24.39600694405854	35.53228520430958	21.160484661499375
30	21.19982839906553	26.822648655977915	32.41101535739451	19.566507587562043
31	29.62450070129656	24.254636226024214	26.0006535061494	20.120209566529816
32	29.19260871208164	25.050624498478484	27.03092595597503	18.72584083346485
33	27.689322287449347	25.021727968062667	26.68305618596918	20.605893558518808
34	21.610159130970192	26.49389505224715	28.54843836481203	23.347507451970632
35	22.428153222741127	24.640516047577027	30.71879008004339	22.21254064963846
36	30.38447945123266	24.003014130403376	25.641669685983626	19.97083673238034
37	22.203427128507318	29.818329736085765	27.442145811892477	20.53609732351444
38	23.422638431051766	30.274005792642946	24.147496782482477	22.15585899382281
39	22.392810543232542	28.075424390005356	26.312513753637074	23.219251313125028
40	24.519817462840173	24.63051340243309	26.89777963505905	23.95188949966769
41	20.18889439651819	23.83585881599801	28.095429680293233	27.87981710719057
42	23.802961227524612	25.827052042651278	24.42001329240399	25.949973437420116
43	23.15101104514305	25.70124099484086	26.344744499100877	24.80300346091522
44	21.71351979745755	27.62508307752495	28.5208755204154	22.140521604602107
45	20.11820903750103	32.14294446753697	25.509857051086843	22.228989443875157
46	23.653588393375134	28.540658529700075	27.172741236015746	20.63301184090904
47	23.009640327108723	27.15962665682703	27.052042651278896	22.778690364785355
48	23.442643721339643	25.040177291328153	28.84184928903421	22.675329698297993
49	22.151635654762035	24.492476899446743	30.71856779904019	22.63731964675103
50	21.21405438327024	29.67851498507383	27.456594077100387	21.65083655455554
51	20.88707902756507	31.314503168615698	25.259124079478795	22.539293724340435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	564.0
1	681.5
2	799.0
3	3288.0
4	5777.0
5	4312.5
6	2848.0
7	2820.5
8	2793.0
9	2870.5
10	2948.0
11	2896.5
12	2845.0
13	2826.5
14	2808.0
15	2652.0
16	2496.0
17	2439.5
18	2383.0
19	2336.5
20	2290.0
21	2290.0
22	2290.0
23	2385.0
24	2480.0
25	2722.0
26	3357.0
27	3750.0
28	4272.0
29	4794.0
30	5535.0
31	6276.0
32	7137.0
33	7998.0
34	8740.0
35	9482.0
36	11245.0
37	13008.0
38	16674.0
39	20340.0
40	25137.0
41	29934.0
42	33115.5
43	36297.0
44	36161.0
45	36025.0
46	36145.0
47	36265.0
48	34953.5
49	33642.0
50	31792.5
51	29943.0
52	27732.0
53	25521.0
54	24037.5
55	22554.0
56	21724.5
57	20895.0
58	19686.0
59	18477.0
60	16997.5
61	15518.0
62	13964.0
63	12410.0
64	10918.5
65	9427.0
66	8094.0
67	6761.0
68	5831.0
69	4901.0
70	4278.0
71	3655.0
72	3075.0
73	2495.0
74	1963.0
75	1110.0
76	789.0
77	624.0
78	459.0
79	369.5
80	280.0
81	222.5
82	165.0
83	117.5
84	70.0
85	44.0
86	18.0
87	13.5
88	9.0
89	5.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	449881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.545729101888924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.50201075602155	27.089619602923094
2	9.256248249785827	7.320901715717275
3	5.170144411104972	6.133713908976067
4	3.389602300961097	5.361771774277876
5	2.549052818549359	5.040207611437968
6	2.026464066411118	4.8082799403003795
7	1.593968313766546	4.412424739324456
8	1.3195889176364302	4.174728469016407
9	1.1120335534936618	3.957855989280413
>10	5.039954063803292	29.46557123212998
>50	0.028596711942106015	0.7641186235994875
>100	0.01065367699803891	0.6641135454673148
>500	5.607198420020478E-4	0.13282270909346297
>1k	0.0011214396840040957	0.6738701384558163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1565	0.34786977000584596	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1474	0.32764219871477124	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	599	0.13314632091597556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0051124630735683435	0.0
2	0.0	0.0	0.0	0.027785125399827953	0.0
3	0.0	0.0	0.0	0.03934373756615638	0.0
4	0.0	0.0	0.0	0.05690393681884765	0.0
5	0.0	0.0	0.0	0.09513626936901091	0.0
6	0.0	0.0	0.0	0.17537971152371404	0.0
7	0.0	0.0	0.0	0.22605978025300025	0.0
8	0.0	0.0	0.0	0.6117173208026122	0.0
9	0.0	0.0	0.0	0.8355542910236262	0.0
10	0.0	0.0	0.0	1.148748224530487	0.0
11	0.0	0.0	0.0	1.3565809625212	0.0
12	0.0	0.0	0.0	1.5499654353040027	0.0
13	0.0	0.0	0.0	1.6368773075546645	0.0
14	0.0	0.0	0.0	1.6675520859960744	0.0
15	0.0	0.0	0.0	1.7055621375430392	0.0
16	0.0	0.0	0.0	1.790695761768112	0.0
17	0.0	0.0	0.0	1.880941849066753	0.0
18	0.0	0.0	0.0	2.0332043362578105	0.0
19	0.0	0.0	0.0	2.094998455147028	0.0
20	0.0	0.0	0.0	2.1736859302793405	0.0
21	0.0	0.0	0.0	2.2568190254756257	0.0
22	0.0	0.0	0.0	2.3415080876943013	0.0
23	0.0	0.0	0.0	2.4353106710441206	0.0
24	0.0	0.0	0.0	2.511997617147646	0.0
25	0.0	0.0	0.0	2.573124893027267	0.0
26	0.0	0.0	0.0	2.640031474990053	0.0
27	0.0	0.0	0.0	2.7151624540711876	0.0
28	0.0	0.0	0.0	2.7885151851267334	0.0
29	0.0	0.0	0.0	2.865646693236656	0.0
30	0.0	0.0	0.0	2.978343161858358	0.0
31	0.0	0.0	0.0	3.067477844141006	0.0
32	0.0	0.0	0.0	3.149054972314901	0.0
33	0.0	0.0	0.0	3.2315212245015905	0.0
34	0.0	0.0	0.0	3.3117646666562934	0.0
35	0.0	0.0	0.0	3.420904639226818	0.0
36	0.0	0.0	0.0	3.512928974551048	0.0
37	0.0	0.0	0.0	3.610954896961641	0.0
38	0.0	0.0	0.0	3.7212062745481584	0.0
39	0.0	0.0	0.0	3.861243306563291	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCT	20	7.0287037E-4	45.0	33
CGGGATA	25	3.887008E-5	45.0	6
ACGTAAG	20	7.0287037E-4	45.0	2
CGTTTTA	895	0.0	43.99441	1
CGTTATT	190	0.0	42.63158	1
GCATAAG	80	0.0	42.1875	2
CGGTCTA	70	0.0	41.785713	31
TCACGAC	65	0.0	41.53846	25
TAAGGCC	65	0.0	41.53846	5
ACGACGG	65	0.0	41.53846	27
CGTTTTT	1520	0.0	41.299343	1
GTTTTAT	1060	0.0	41.179245	2
AGGCGAT	385	0.0	40.909092	7
CGCAGTA	95	0.0	40.263157	37
GTTGATC	325	0.0	40.153847	16
CGCATCG	45	1.9237632E-8	40.000004	21
CGCCAGT	45	1.9237632E-8	40.000004	26
GGTACCT	340	0.0	39.705883	8
CAGTAGC	335	0.0	39.626865	27
ATCCTGC	330	0.0	39.545452	20
>>END_MODULE