FastQCFastQC Report
Thu 26 May 2016
SRR1545688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545688_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1167223
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67160.5753827674745957No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.25325066418327946No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.22686324721154397No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12140.10400754611586645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGC408.312782E-944.00000413
ACCGGTC254.442882E-544.034
TATCGCG254.442882E-544.032
GCGTAAA502.7284841E-1144.01
CGTTTTA39550.043.4437451
CGTTATT22000.043.41
TAGCCGT1300.042.3076944
GTTTTAT43300.041.9676672
GTTATTT24700.040.971662
ATTACGG650.040.6153832
CGTTTTT20150.039.9602971
AGGCGAT9450.039.576727
GGTACCT9250.039.2432448
TCGTTAA452.3510438E-839.111111
CGTAGGT452.3510438E-839.111114
AGGTACC5400.038.7037057
ATAGGCG2400.038.5000045
CGTAAAG1200.038.5000042
TCGAATA404.124613E-738.5000041
TGCGTAA404.124613E-738.5000041