##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545688_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1167223 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.520384707977826 31.0 31.0 33.0 30.0 34.0 2 31.718435980099777 31.0 31.0 34.0 30.0 34.0 3 31.728782760449374 31.0 31.0 34.0 30.0 34.0 4 35.653058584349345 37.0 35.0 37.0 33.0 37.0 5 27.07928305045394 35.0 25.0 37.0 0.0 37.0 6 31.330953896556185 35.0 26.0 37.0 17.0 37.0 7 15.017624738374758 0.0 0.0 32.0 0.0 37.0 8 25.113512156631593 17.0 17.0 35.0 17.0 37.0 9 33.218850211142176 32.0 32.0 37.0 28.0 39.0 10 36.030129632469546 37.0 35.0 39.0 32.0 39.0 11 36.99809033920682 39.0 37.0 39.0 34.0 39.0 12 37.092108363183385 39.0 37.0 39.0 34.0 39.0 13 36.6891236721689 39.0 35.0 39.0 33.0 39.0 14 38.002187242711976 40.0 37.0 41.0 33.0 41.0 15 38.271323474605964 40.0 37.0 41.0 33.0 41.0 16 38.308163050248325 40.0 37.0 41.0 34.0 41.0 17 38.3217491430515 40.0 37.0 41.0 34.0 41.0 18 38.28351908761222 40.0 37.0 41.0 34.0 41.0 19 38.21803888374372 40.0 37.0 41.0 34.0 41.0 20 38.244284082818794 40.0 37.0 41.0 34.0 41.0 21 38.110302829879124 40.0 36.0 41.0 34.0 41.0 22 38.00884663856007 40.0 36.0 41.0 34.0 41.0 23 37.88929364825744 40.0 36.0 41.0 34.0 41.0 24 37.72367919412143 40.0 35.0 41.0 33.0 41.0 25 37.54795870197897 39.0 35.0 41.0 33.0 41.0 26 37.30074030412355 39.0 35.0 40.0 33.0 41.0 27 37.08669380229828 39.0 35.0 40.0 33.0 41.0 28 37.11451025211121 39.0 35.0 40.0 33.0 41.0 29 37.02216286005331 39.0 35.0 40.0 33.0 41.0 30 36.787272012288994 38.0 35.0 40.0 32.0 41.0 31 36.58982045418913 38.0 35.0 40.0 31.0 41.0 32 36.32699578401043 38.0 35.0 40.0 31.0 41.0 33 36.06381728255869 38.0 35.0 40.0 30.0 41.0 34 35.76127612290025 38.0 35.0 40.0 29.0 41.0 35 35.502983577259876 38.0 35.0 40.0 27.0 41.0 36 35.27713384674565 38.0 35.0 40.0 26.0 41.0 37 35.12911928568919 38.0 34.0 40.0 25.0 41.0 38 34.95188494400813 38.0 34.0 40.0 25.0 41.0 39 34.850598386083895 38.0 34.0 40.0 24.0 41.0 40 34.62200967595738 38.0 34.0 40.0 24.0 41.0 41 34.4015462340958 37.0 34.0 40.0 23.0 40.0 42 34.40438545162321 37.0 34.0 40.0 23.0 40.0 43 34.29212584056346 37.0 33.0 40.0 23.0 40.0 44 34.06765288209708 37.0 33.0 40.0 23.0 40.0 45 33.87593287657971 37.0 33.0 40.0 23.0 40.0 46 33.814545292544786 36.0 33.0 39.0 23.0 40.0 47 33.63778986534707 36.0 33.0 39.0 23.0 40.0 48 33.53126180686981 36.0 33.0 39.0 22.0 40.0 49 33.41525226970339 36.0 33.0 39.0 22.0 40.0 50 33.49354322181794 36.0 33.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 1.0 12 3.0 13 1.0 14 10.0 15 23.0 16 60.0 17 172.0 18 432.0 19 992.0 20 1844.0 21 3117.0 22 5098.0 23 7701.0 24 11790.0 25 17033.0 26 21138.0 27 22219.0 28 22310.0 29 23712.0 30 28049.0 31 36759.0 32 50759.0 33 86689.0 34 93703.0 35 119942.0 36 164044.0 37 293110.0 38 124180.0 39 32331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.58150070723418 22.4349588724691 26.7660078665345 17.217532553762222 2 36.51461631581969 22.66190779311237 28.626492109905303 12.19698378116264 3 29.73870460057761 22.99757629861646 34.69294213702095 12.570776963784983 4 25.406884545626674 26.748958853620948 33.27890214637649 14.565254454375898 5 16.881264334236047 47.63125812291224 24.566342506958826 10.921135035892885 6 19.873408937281052 39.76600872326882 30.34141719277293 10.0191651466772 7 38.45597627874022 55.61867783619754 4.9445564386582515 0.9807894464039861 8 85.61011906036808 2.9283178964088266 9.7314737629399 1.7300892802832022 9 81.48245879322118 4.405670553099108 11.336394159470812 2.7754764942089043 10 45.215181674795645 24.638565209904193 17.49160186185502 12.654651253445143 11 37.58904682310064 22.791531695314436 24.66932197189397 14.95009950969095 12 34.010210559593155 21.088772239751957 28.89002358589575 16.010993614759133 13 22.795815366900754 31.231735495273828 29.61207926848597 16.36036986933945 14 17.795057157029976 33.74770716478342 32.177484508101706 16.279751170084893 15 16.85864654826027 22.659423263592306 43.543864368676765 16.93806581947066 16 19.005708420755933 18.700282636651266 41.80812064189962 20.485888300693183 17 19.65511303324215 18.995770302675666 32.06285345645177 29.28626320763042 18 22.276720044070412 22.189761510868102 35.23833920339129 20.295179241670187 19 27.709615043569226 24.02891306973903 28.285854545361083 19.97561734133066 20 29.11825760801492 23.705324518108366 27.548377645060114 19.6280402288166 21 23.83306360481245 25.9479979404107 30.643330366176812 19.575608088600035 22 22.939918079064583 22.740556003437217 29.16023758956086 25.159288327937336 23 20.54645941692376 27.590100606310873 28.61920986820856 23.244230108556803 24 20.49428429700237 24.350616805871713 36.80676271800676 18.34833617911916 25 20.952894177033865 23.930645643548836 34.16399436954207 20.952465809875235 26 18.97520867906133 29.17257456372947 29.789080578432742 22.063136178776464 27 19.427050357986435 27.78372256201257 31.58376762623766 21.205459453763336 28 18.249297692043424 25.86181046809393 36.57510175861853 19.313790081244115 29 18.980092064669734 24.162135256073604 36.004088336161985 20.853684343094677 30 21.065726086617552 26.485855744789127 33.08219594713264 19.36622222146068 31 27.564055883066047 24.57233964717967 27.795802515886 20.067801953868283 32 27.828872460532395 24.59135914902294 28.06258958228205 19.517178808162623 33 26.374308936681334 25.022382184038527 27.575107755758754 21.028201123521384 34 21.200576068154927 26.39247170420734 29.412545845995155 22.99440638164258 35 21.62003318988745 25.305704222757775 31.100312451005507 21.973950136349266 36 28.33563080919413 24.82490492390914 26.60596989606956 20.233494370827167 37 21.856663208315805 29.924701620855654 27.727520790800043 20.491114380028495 38 23.24482982257889 29.887348004622936 25.33877416740417 21.529048005394 39 21.777757977695778 28.38917670402314 26.1841139182487 23.648951400032384 40 24.019403318817396 25.514147682148142 27.43674516352059 23.029703835513864 41 20.498824988883875 24.037908780070303 28.79920974826576 26.66405648278007 42 22.39177946287899 26.197307626734567 25.966760421958785 25.44415248842766 43 21.85820533008688 25.576860634171876 27.569110615537905 24.995823420203337 44 21.58250822679128 27.18889192553608 29.41794327219392 21.81065657547872 45 19.937835357939313 30.86582426837031 26.984903484595492 22.211436889094884 46 22.533911686113107 28.79329828147663 28.07886753431007 20.59392249810019 47 21.99322665848771 27.23764010818841 28.204036418062355 22.565096815261523 48 22.362821842955462 25.641115707966687 29.761750753712015 22.23431169536584 49 22.139985247035057 24.48495274681873 30.967775652124747 22.40728635402147 50 20.919738558955743 28.86406453608265 28.486844416191246 21.729352488770353 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1208.0 1 1595.5 2 1983.0 3 11338.5 4 20694.0 5 14878.0 6 9062.0 7 9179.5 8 9297.0 9 9417.0 10 9537.0 11 9448.0 12 9359.0 13 8919.0 14 8479.0 15 8105.5 16 7732.0 17 7098.0 18 6464.0 19 6352.5 20 6241.0 21 6202.5 22 6164.0 23 6873.0 24 7582.0 25 8515.5 26 9449.0 27 10739.5 28 12030.0 29 13953.0 30 15876.0 31 18211.5 32 20547.0 33 24279.5 34 28012.0 35 30538.5 36 33065.0 37 37463.0 38 41861.0 39 51930.0 40 61999.0 41 72828.5 42 83658.0 43 87448.0 44 91238.0 45 92595.5 46 93953.0 47 92441.5 48 90930.0 49 87221.5 50 83513.0 51 76363.0 52 69213.0 53 63806.0 54 58399.0 55 56158.5 56 53918.0 57 51896.5 58 49875.0 59 46688.0 60 43501.0 61 39426.0 62 35351.0 63 31144.5 64 26938.0 65 23195.0 66 19452.0 67 16868.5 68 14285.0 69 12454.0 70 10623.0 71 8720.0 72 6817.0 73 5485.0 74 4153.0 75 3325.0 76 2497.0 77 1824.5 78 1152.0 79 869.0 80 586.0 81 445.5 82 305.0 83 220.0 84 135.0 85 96.0 86 57.0 87 39.0 88 21.0 89 14.5 90 8.0 91 5.0 92 2.0 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1167223.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.831396676225857 #Duplication Level Percentage of deduplicated Percentage of total 1 69.6564993249281 20.779506624391885 2 7.849504035576461 4.683233371938342 3 4.146756084610205 3.7111057703868062 4 2.726326332445218 3.2532048916805314 5 2.0620689865859863 3.07571989562948 6 1.6253416912893277 2.9091727636355853 7 1.3925819716871446 2.907986564109195 8 1.1327319207684607 2.7032780205013753 9 0.9753338245460779 2.6186013190597066 >10 8.20808410694155 46.73831367229443 >50 0.19390765051419068 3.7185135445733106 >100 0.029186674992787126 1.4589382223563405 >500 3.354790228947982E-4 0.08796863063695567 >1k 0.0010064370686843945 0.6823323363853968 >5k 3.354790228947982E-4 0.6721243724206989 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6716 0.5753827674745957 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2956 0.25325066418327946 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2648 0.22686324721154397 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 1214 0.10400754611586645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004455018449773522 0.0 2 0.0 0.0 0.0 0.01722035977700919 0.0 3 0.0 0.0 0.0 0.026473090403461894 0.0 4 0.0 0.0 0.0 0.04180863468248998 0.0 5 0.0 0.0 0.0 0.07513559962406498 0.0 6 0.0 0.0 0.0 0.1684339667741297 0.0 7 0.0 0.0 0.0 0.23902887451669474 0.0 8 0.0 0.0 0.0 0.6256730718980006 0.0 9 0.0 0.0 0.0 0.8513368910653748 0.0 10 0.0 0.0 0.0 1.1632738559812479 0.0 11 0.0 0.0 0.0 1.3495278965544717 0.0 12 0.0 0.0 0.0 1.5250727581618937 0.0 13 0.0 0.0 0.0 1.6000370109225057 0.0 14 0.0 0.0 0.0 1.6369622599965903 0.0 15 0.0 0.0 0.0 1.670460571801618 0.0 16 0.0 0.0 0.0 1.7365147876626832 0.0 17 0.0 0.0 0.0 1.810193938947399 0.0 18 0.0 0.0 0.0 1.9336493540651616 0.0 19 0.0 0.0 0.0 1.9871095754624437 0.0 20 0.0 0.0 0.0 2.053163791323509 0.0 21 0.0 0.0 0.0 2.119389354048027 0.0 22 0.0 0.0 0.0 2.194439280240365 0.0 23 0.0 0.0 0.0 2.280284058830232 0.0 24 8.567343172641389E-5 0.0 0.0 2.3423972968318822 0.0 25 8.567343172641389E-5 0.0 0.0 2.3935443355725514 0.0 26 8.567343172641389E-5 0.0 0.0 2.4476899444236446 0.0 27 8.567343172641389E-5 0.0 0.0 2.5123733853770873 0.0 28 8.567343172641389E-5 0.0 0.0 2.572344787585577 0.0 29 8.567343172641389E-5 0.0 0.0 2.642254307874331 0.0 30 8.567343172641389E-5 0.0 0.0 2.7358953687513012 0.0 31 8.567343172641389E-5 0.0 0.0 2.817799169481753 0.0 32 8.567343172641389E-5 0.0 0.0 2.893620156559629 0.0 33 8.567343172641389E-5 0.0 0.0 2.9708975919768545 0.0 34 8.567343172641389E-5 0.0 0.0 3.054257841046655 0.0 35 8.567343172641389E-5 0.0 0.0 3.157751346572163 0.0 36 8.567343172641389E-5 0.0 0.0 3.241968329959228 0.0 37 8.567343172641389E-5 0.0 0.0 3.330211964637434 0.0 38 8.567343172641389E-5 0.0 0.0 3.449383708168876 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTCGC 40 8.312782E-9 44.000004 13 ACCGGTC 25 4.442882E-5 44.0 34 TATCGCG 25 4.442882E-5 44.0 32 GCGTAAA 50 2.7284841E-11 44.0 1 CGTTTTA 3955 0.0 43.443745 1 CGTTATT 2200 0.0 43.4 1 TAGCCGT 130 0.0 42.30769 44 GTTTTAT 4330 0.0 41.967667 2 GTTATTT 2470 0.0 40.97166 2 ATTACGG 65 0.0 40.615383 2 CGTTTTT 2015 0.0 39.960297 1 AGGCGAT 945 0.0 39.57672 7 GGTACCT 925 0.0 39.243244 8 TCGTTAA 45 2.3510438E-8 39.11111 1 CGTAGGT 45 2.3510438E-8 39.11111 4 AGGTACC 540 0.0 38.703705 7 ATAGGCG 240 0.0 38.500004 5 CGTAAAG 120 0.0 38.500004 2 TCGAATA 40 4.124613E-7 38.500004 1 TGCGTAA 40 4.124613E-7 38.500004 1 >>END_MODULE