FastQCFastQC Report
Thu 26 May 2016
SRR1545687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545687_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences442689
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.41338275855058515No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.3704632371710162No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4750.10729880344892237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA207.0285815E-445.01
CGTAGTG207.0285815E-445.016
ATGCGAA207.0285815E-445.01
CACGTGA253.8869053E-545.043
AGTACGG207.0285815E-445.02
GTTACAC302.1624674E-644.99999633
AACACGT302.1624674E-644.99999641
CGTTTTA9700.043.8402061
AGGCGAT4500.043.07
TATCCTA1000.042.7524
CGTTTTT14700.042.7040821
GTACGGG800.042.18753
CGTTATT2350.042.127661
CGAAAAG603.6379788E-1241.2499962
GTAAGGC950.040.2631574
TCACGAC451.9237632E-840.025
TAGGCGA1350.040.06
CGACGGT403.4518052E-739.37528
CACGACG403.4518052E-739.37526
GTTTTAT11550.039.350652