##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545687_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 442689 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.009837606084634 31.0 31.0 33.0 30.0 33.0 2 31.17939908152224 31.0 31.0 33.0 30.0 34.0 3 31.079814497310753 31.0 31.0 34.0 28.0 34.0 4 35.05418250735844 35.0 35.0 37.0 32.0 37.0 5 32.83407764819094 35.0 35.0 37.0 28.0 37.0 6 34.01990336330923 35.0 35.0 37.0 28.0 37.0 7 15.441115546128321 0.0 0.0 35.0 0.0 37.0 8 24.976035094614957 17.0 17.0 35.0 17.0 37.0 9 32.72555676784379 32.0 32.0 37.0 27.0 39.0 10 35.49453227886846 37.0 34.0 37.0 32.0 39.0 11 36.443528075014285 37.0 35.0 39.0 32.0 39.0 12 36.40611806482655 37.0 35.0 39.0 32.0 39.0 13 36.194079816756236 38.0 35.0 39.0 32.0 39.0 14 37.22977756393315 39.0 36.0 40.0 32.0 41.0 15 37.54173471669728 39.0 36.0 41.0 32.0 41.0 16 37.538179173189306 39.0 36.0 41.0 32.0 41.0 17 37.487870717365915 39.0 36.0 41.0 32.0 41.0 18 37.40915405623361 39.0 36.0 40.0 32.0 41.0 19 37.338777335782005 39.0 36.0 40.0 32.0 41.0 20 37.43153093932761 39.0 35.0 41.0 32.0 41.0 21 37.301539003679785 39.0 35.0 40.0 32.0 41.0 22 37.346631608194464 39.0 35.0 41.0 32.0 41.0 23 37.30384762214557 39.0 35.0 41.0 32.0 41.0 24 37.11377513333288 39.0 35.0 40.0 32.0 41.0 25 36.86922873620081 38.0 35.0 40.0 31.0 41.0 26 36.743025013045276 38.0 35.0 40.0 31.0 41.0 27 36.659438115697476 38.0 35.0 40.0 31.0 41.0 28 36.56764681299965 38.0 35.0 40.0 31.0 41.0 29 36.522757511480975 38.0 35.0 40.0 31.0 41.0 30 36.22340514446937 38.0 35.0 40.0 30.0 41.0 31 36.07248203592138 38.0 35.0 40.0 30.0 41.0 32 35.9447625759845 38.0 35.0 40.0 30.0 41.0 33 35.691304730860715 38.0 34.0 40.0 28.0 41.0 34 35.46756300698684 38.0 34.0 40.0 27.0 41.0 35 35.24163012860044 38.0 34.0 40.0 25.0 41.0 36 35.002094020858884 38.0 34.0 40.0 24.0 41.0 37 34.85049549457972 38.0 34.0 40.0 24.0 41.0 38 34.717569219022835 38.0 34.0 40.0 23.0 41.0 39 34.593138749776934 38.0 34.0 40.0 23.0 41.0 40 34.44495345490852 38.0 33.0 40.0 23.0 41.0 41 34.26353037911491 38.0 33.0 40.0 22.0 41.0 42 34.22630334162358 37.0 33.0 40.0 22.0 41.0 43 34.150819198127806 37.0 33.0 40.0 22.0 41.0 44 33.93474651504781 37.0 33.0 40.0 21.0 41.0 45 33.86668293090635 37.0 33.0 40.0 21.0 41.0 46 33.82004070577764 37.0 33.0 40.0 21.0 41.0 47 33.73997095026079 37.0 33.0 40.0 20.0 41.0 48 33.66084542421429 36.0 33.0 40.0 20.0 41.0 49 33.54374515743558 36.0 33.0 40.0 20.0 41.0 50 33.43043536207134 36.0 32.0 40.0 20.0 41.0 51 33.25769106528511 36.0 32.0 40.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 0.0 12 2.0 13 3.0 14 6.0 15 12.0 16 50.0 17 157.0 18 314.0 19 532.0 20 1092.0 21 1745.0 22 2715.0 23 3986.0 24 5536.0 25 7314.0 26 8556.0 27 9054.0 28 9318.0 29 10510.0 30 12534.0 31 16219.0 32 21170.0 33 30685.0 34 39206.0 35 43922.0 36 53856.0 37 80451.0 38 69075.0 39 14668.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.47993738267723 23.253344899014884 26.800304502709576 16.466413215598312 2 35.978305311403716 22.593739623076246 29.19453611903616 12.233418946483875 3 30.2761080578013 22.93031902757918 34.07516337654651 12.718409538073002 4 25.94417299729607 27.448389275541068 31.81353049206102 14.793907235101845 5 20.73780916173657 36.02845338375248 29.733063166240857 13.500674288270096 6 20.0151799570353 40.38252588160085 29.774853226531494 9.827440934832355 7 37.54509373397577 56.35717173907642 5.347320579458717 0.7504139474890951 8 86.32312978185588 3.22799979217916 8.812055415878868 1.6368150100860877 9 81.92297527157892 4.618140500441619 10.444126689391425 3.014757538588038 10 45.37316264917357 25.46189311232039 16.617083324862374 12.547860913643664 11 37.94695598941921 23.312076875639555 23.928762630198626 14.812204504742606 12 34.42958826625464 21.646799446112283 28.056265233606435 15.867347054026643 13 23.11148458624452 32.10673859074881 28.581238747743903 16.20053807526277 14 17.801210330502904 34.7833354793094 31.34390057128142 16.071553618906275 15 16.870760285437406 23.27322341418016 42.91726245739108 16.938753842991357 16 18.986015012796795 19.159726128275153 41.272993004118014 20.581265854810034 17 19.51979832342796 19.50195283822277 31.23727944448586 29.740969393863416 18 22.235700457883524 22.43403382510069 35.01035715818554 20.319908558830242 19 28.15385067169051 24.31639367592147 27.557947001167864 19.971808651220158 20 29.663036578726825 23.946608115403816 26.81272857468787 19.577626731181482 21 23.956547372986453 26.66454327981947 29.871760987962205 19.507148359231877 22 22.802915816747195 23.173830838353787 28.523636232208165 25.499617112690853 23 20.66077991547113 28.061234862397754 27.981946693954445 23.296038528176666 24 20.329170139759515 24.899421490030246 36.45222718432127 18.319181185888965 25 20.933657714558077 24.50072172563583 33.92426737506466 20.641353184741433 26 18.820210124940985 30.067157756348134 29.15116481322102 21.961467305489858 27 19.448190490389415 28.967062655724447 30.56457241991556 21.020174433970578 28 18.232438574258676 26.57508996157573 35.96701070051436 19.22546076365123 29 18.769384375938863 24.723903236809587 35.63043129601142 20.876281091240127 30 20.913779199392803 27.470526712884215 32.40672345597022 19.208970631752763 31 27.901077279986623 25.046477323809718 27.14343478152834 19.909010614675314 32 28.51188983688323 24.62451066098322 27.460587455301578 19.403012046831975 33 26.867620383610163 25.355723769960402 26.82605621553732 20.950599630892118 34 21.172877573194725 26.550693602054714 28.73213474922575 23.544294075524803 35 21.60094332590148 25.46257078897375 31.073507586590132 21.86297829853464 36 28.58575659209965 25.139996701973622 26.182941071497147 20.091305634429588 37 21.766748213757285 30.608847294601855 27.4337062813849 20.19069821025596 38 23.011640225982575 30.590775917178874 25.103853947127668 21.293729909710883 39 21.881049675957616 28.873769169778328 25.96924703347045 23.275934120793604 40 24.30103300511194 25.603075748437394 27.052626109977883 23.043265136472783 41 20.383158379810656 24.337853436611255 28.5954699574645 26.68351822611359 42 22.242477224417144 26.284140785065812 25.604205209526327 25.869176780990717 43 21.67503597333568 25.67920142583168 27.267223716875733 25.378538883956907 44 21.299151322937774 27.305399501681766 29.514399499422844 21.881049675957616 45 19.673630923741044 31.69945492207848 26.577348883753604 22.049565270426868 46 22.291721727894753 29.55438242197118 27.590023696093645 20.56387215404042 47 21.799502585336434 27.50147394672106 27.8432488722331 22.855774595709402 48 22.105586540438097 26.009907632672146 29.876278832317947 22.00822699457181 49 22.098809773904478 25.000847095816702 30.909283944258835 21.99105918601998 50 21.055639512163165 29.65309732114419 28.019670694324912 21.271592472367733 51 20.242427528129227 31.175836761247737 26.598808644443388 21.982927066179645 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 478.0 1 631.0 2 784.0 3 3690.0 4 6596.0 5 4773.5 6 2951.0 7 3006.0 8 3061.0 9 3086.5 10 3112.0 11 3068.5 12 3025.0 13 2924.5 14 2824.0 15 2728.5 16 2633.0 17 2484.0 18 2335.0 19 2287.0 20 2239.0 21 2241.5 22 2244.0 23 2510.5 24 2777.0 25 3143.0 26 3927.0 27 4345.0 28 4935.0 29 5525.0 30 6440.0 31 7355.0 32 8668.0 33 9981.0 34 11153.0 35 12325.0 36 13764.5 37 15204.0 38 18272.5 39 21341.0 40 25636.5 41 29932.0 42 32168.5 43 34405.0 44 34581.0 45 34757.0 46 34379.5 47 34002.0 48 32549.5 49 31097.0 50 29640.5 51 28184.0 52 26270.0 53 24356.0 54 22661.0 55 20966.0 56 20072.5 57 19179.0 58 18397.0 59 17615.0 60 15910.5 61 14206.0 62 12569.5 63 10933.0 64 9737.5 65 8542.0 66 7404.0 67 6266.0 68 5462.5 69 4659.0 70 4103.5 71 3548.0 72 2938.0 73 2328.0 74 1890.0 75 1109.0 76 766.0 77 593.0 78 420.0 79 325.0 80 230.0 81 172.5 82 115.0 83 84.0 84 53.0 85 39.0 86 25.0 87 15.5 88 6.0 89 3.0 90 0.0 91 1.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 442689.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.77423301881253 #Duplication Level Percentage of deduplicated Percentage of total 1 67.60531851698353 28.24160329039512 2 10.294547334191423 8.600936383234156 3 5.607334062441239 7.027262392162349 4 3.777746606040433 6.3124986802704495 5 2.73677079117868 5.716325037488905 6 2.035771206042862 5.1025668440534115 7 1.6240494980562645 4.749039551612154 8 1.2921949468081857 4.318436225095777 9 0.9585552355586554 3.6038618794467 >10 4.0328021454848 24.226136787584135 >50 0.026316510822752284 0.7482324703259993 >100 0.007519003092208873 0.5745797170925664 >500 0.0 0.0 >1k 0.0010741432988869818 0.7785207412382683 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1830 0.41338275855058515 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1640 0.3704632371710162 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 475 0.10729880344892237 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0045178443557441 0.0 2 0.0 0.0 0.0 0.02078208403642286 0.0 3 0.0 0.0 0.0 0.031399018272421496 0.0 4 0.0 0.0 0.0 0.04879271904203628 0.0 5 0.0 0.0 0.0 0.0901309948970948 0.0 6 0.0 0.0 0.0 0.16512721120244686 0.0 7 0.0 0.0 0.0 0.20827262479980302 0.0 8 0.0 0.0 0.0 0.5301690351465702 0.0 9 0.0 0.0 0.0 0.7255658035325026 0.0 10 0.0 0.0 0.0 0.9907632672146812 0.0 11 0.0 0.0 0.0 1.1423369453498957 0.0 12 0.0 0.0 0.0 1.2882633180404301 0.0 13 0.0 0.0 0.0 1.356256875594379 0.0 14 0.0 0.0 0.0 1.3851710794711412 0.0 15 0.0 0.0 0.0 1.418151343268073 0.0 16 0.0 0.0 0.0 1.4800458109417673 0.0 17 0.0 0.0 0.0 1.545780446317844 0.0 18 0.0 0.0 0.0 1.6465283754509374 0.0 19 0.0 0.0 0.0 1.691255034572804 0.0 20 0.0 0.0 0.0 1.7558602088599446 0.0 21 0.0 0.0 0.0 1.8200135987115107 0.0 22 0.0 0.0 0.0 1.8837152041275027 0.0 23 0.0 0.0 0.0 1.9575819593439185 0.0 24 0.0 0.0 0.0 2.01405501379072 0.0 25 0.0 0.0 0.0 2.057426319605863 0.0 26 0.0 0.0 0.0 2.1098333141324948 0.0 27 0.0 0.0 0.0 2.157270679867808 0.0 28 0.0 0.0 0.0 2.214647303185758 0.0 29 0.0 0.0 0.0 2.2801560463440476 0.0 30 0.0 0.0 0.0 2.3705129334589294 0.0 31 0.0 0.0 0.0 2.441668982061899 0.0 32 0.0 0.0 0.0 2.5019822042110826 0.0 33 0.0 0.0 0.0 2.567265055151585 0.0 34 0.0 0.0 0.0 2.642487163674724 0.0 35 0.0 0.0 0.0 2.737813679580925 0.0 36 0.0 0.0 0.0 2.814391141410787 0.0 37 0.0 0.0 0.0 2.8945828787252452 0.0 38 0.0 0.0 0.0 2.991264747938169 0.0 39 0.0 0.0 0.0 3.170171384425635 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTAA 20 7.0285815E-4 45.0 1 CGTAGTG 20 7.0285815E-4 45.0 16 ATGCGAA 20 7.0285815E-4 45.0 1 CACGTGA 25 3.8869053E-5 45.0 43 AGTACGG 20 7.0285815E-4 45.0 2 GTTACAC 30 2.1624674E-6 44.999996 33 AACACGT 30 2.1624674E-6 44.999996 41 CGTTTTA 970 0.0 43.840206 1 AGGCGAT 450 0.0 43.0 7 TATCCTA 100 0.0 42.75 24 CGTTTTT 1470 0.0 42.704082 1 GTACGGG 80 0.0 42.1875 3 CGTTATT 235 0.0 42.12766 1 CGAAAAG 60 3.6379788E-12 41.249996 2 GTAAGGC 95 0.0 40.263157 4 TCACGAC 45 1.9237632E-8 40.0 25 TAGGCGA 135 0.0 40.0 6 CGACGGT 40 3.4518052E-7 39.375 28 CACGACG 40 3.4518052E-7 39.375 26 GTTTTAT 1155 0.0 39.35065 2 >>END_MODULE