FastQCFastQC Report
Thu 26 May 2016
SRR1545686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545686_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1340176
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC48700.3633851076276549No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37160.27727701436229274No Hit
ATGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT21880.1632621387041702No Hit
ATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG18840.14057855087689974No Hit
ATGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC17030.1270728620718473No Hit
ATGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC16110.12020809207148912No Hit
ATTGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC15120.11282100261458196No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT15090.11259715141891811No Hit
ATATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC14090.10513544489678966No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTA253.8911527E-545.00000440
CGAACTA253.8911527E-545.00000418
CGAAACG253.8911527E-545.00000442
TCCGATA253.8911527E-545.0000042
GAACTAC253.8911527E-545.00000430
ATTCGTT2300.045.0000041
CGTCTAG253.8911527E-545.0000043
CGTTAAT253.8911527E-545.00000424
ATCCGAT253.8911527E-545.0000041
TCTACGA253.8911527E-545.00000425
ATCCCGC253.8911527E-545.0000044
AACGCGC253.8911527E-545.0000041
TCGTAAT253.8911527E-545.0000042
GTGCGAC253.8911527E-545.00000414
CTTACCG253.8911527E-545.00000412
AGCGTAA207.033706E-445.026
CGTATGA207.033706E-445.025
AGGAACG207.033706E-445.033
CCCACGT207.033706E-445.022
CATTACG207.033706E-445.022