##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545686_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1340176
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865384098804935	31.0	31.0	34.0	31.0	34.0
2	32.536874261291054	34.0	31.0	34.0	31.0	34.0
3	32.50895852485047	34.0	31.0	34.0	31.0	34.0
4	35.994200761691005	37.0	35.0	37.0	35.0	37.0
5	35.76270430152458	37.0	35.0	37.0	35.0	37.0
6	35.77923198147109	37.0	35.0	37.0	35.0	37.0
7	35.761806658229965	37.0	35.0	37.0	35.0	37.0
8	35.73045331359463	37.0	35.0	37.0	33.0	37.0
9	37.91102288057688	39.0	38.0	39.0	35.0	39.0
10	37.85104195269875	39.0	38.0	39.0	35.0	39.0
11	37.65662047372882	39.0	38.0	39.0	35.0	39.0
12	37.17458900920476	39.0	37.0	39.0	33.0	39.0
13	36.92999277706809	39.0	37.0	39.0	33.0	39.0
14	37.60417064624348	40.0	35.0	41.0	33.0	41.0
15	37.479896670288085	40.0	35.0	41.0	32.0	41.0
16	37.50804595814281	40.0	35.0	41.0	32.0	41.0
17	37.60387963968912	40.0	35.0	41.0	33.0	41.0
18	37.667954059765286	40.0	35.0	41.0	33.0	41.0
19	37.632947463616716	40.0	35.0	41.0	33.0	41.0
20	37.56474149663925	39.0	35.0	41.0	33.0	41.0
21	37.543955420780556	39.0	35.0	41.0	32.0	41.0
22	37.47029942335932	39.0	35.0	41.0	32.0	41.0
23	37.389238428385525	39.0	35.0	41.0	32.0	41.0
24	37.374829873091294	39.0	35.0	41.0	32.0	41.0
25	37.28664966392474	39.0	35.0	41.0	32.0	41.0
26	37.17607761965593	39.0	35.0	41.0	32.0	41.0
27	37.08877341483507	39.0	35.0	41.0	32.0	41.0
28	36.97416234882583	39.0	35.0	41.0	31.0	41.0
29	36.919826201931684	39.0	35.0	41.0	31.0	41.0
30	36.849172049044306	39.0	35.0	41.0	31.0	41.0
31	36.76818641730638	39.0	35.0	41.0	31.0	41.0
32	36.698220979930994	39.0	35.0	41.0	31.0	41.0
33	36.5897971609699	39.0	35.0	41.0	31.0	41.0
34	36.500483518582634	39.0	35.0	41.0	30.0	41.0
35	36.41501862441948	38.0	35.0	41.0	30.0	41.0
36	36.29549477083607	38.0	35.0	41.0	30.0	41.0
37	36.1674712873533	38.0	35.0	41.0	30.0	41.0
38	36.09018740822101	38.0	35.0	41.0	30.0	41.0
39	36.00852723821349	38.0	35.0	40.0	30.0	41.0
40	35.95733246976516	38.0	35.0	40.0	30.0	41.0
41	35.8842890784494	38.0	35.0	40.0	30.0	41.0
42	35.697943404448374	38.0	35.0	40.0	29.0	41.0
43	35.59259455474505	37.0	34.0	40.0	29.0	41.0
44	35.50925923162331	37.0	34.0	40.0	29.0	41.0
45	35.4760755303781	37.0	34.0	40.0	28.0	41.0
46	35.38910262532682	37.0	34.0	40.0	28.0	41.0
47	35.287004841155195	37.0	34.0	40.0	28.0	41.0
48	35.22699332028032	37.0	34.0	40.0	28.0	41.0
49	35.192519489977435	36.0	34.0	40.0	28.0	41.0
50	35.06802315516768	36.0	34.0	40.0	28.0	41.0
51	34.91788093504137	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	8.0
11	12.0
12	19.0
13	21.0
14	21.0
15	42.0
16	90.0
17	183.0
18	435.0
19	785.0
20	1482.0
21	2407.0
22	3550.0
23	4922.0
24	7031.0
25	9944.0
26	13269.0
27	16125.0
28	18827.0
29	22257.0
30	27253.0
31	33419.0
32	41403.0
33	53665.0
34	86803.0
35	165646.0
36	100775.0
37	138266.0
38	206761.0
39	384547.0
40	202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.1997110827234634	90.80993839615095	3.512598345291962	3.477752175833622
2	3.6060189109490097	2.732476928403434	90.8297119184346	2.831792242212963
3	28.501405785508773	15.853365528109741	42.77818734255799	12.867041343823498
4	27.07510058380392	15.936041236374923	43.38564487052447	13.603213309296688
5	23.92849894342236	14.317298623464382	46.80295722352885	14.951245209584412
6	23.417222812526116	15.059066868829168	47.05404364799847	14.469666670646244
7	23.189043827079427	17.31787466720789	44.93469514451833	14.55838636119435
8	26.21304962930242	20.647138883251152	42.38480617471138	10.755005312735044
9	83.20705638662385	3.9122473466171606	9.064854168407733	3.815842098351261
10	83.81249925382934	4.25093420565657	8.521343465335898	3.4152230751781856
11	77.52034061197932	6.850443523835676	9.732826136268669	5.8963897279163335
12	44.61749800026265	22.561962010959753	15.239640166664678	17.580899822112915
13	35.94938276763649	23.24023113382123	22.71164384379365	18.09874225474863
14	30.20163023364095	23.14016964935949	26.447944150619023	20.210255966380537
15	23.47385716502907	25.475609173720464	30.97100679313762	20.079526868112847
16	18.436608326070605	31.880066498728528	29.50948233664832	20.173842838552545
17	18.12612671768484	22.980265278590277	37.9392706629577	20.954337340767182
18	21.64215744797698	20.493726197156196	36.7077160014804	21.15640035338642
19	22.638742971072457	20.475071930850874	29.104908609018516	27.781276489058154
20	22.57681080693879	21.566421126777378	31.797763875789446	24.05900419049438
21	27.494970809804087	23.556980575685582	27.208888981745684	21.73915963276465
22	28.646386743233727	22.38206026671124	26.615384844975587	22.35616814507945
23	26.400786165399172	23.56556153818603	27.84843184775731	22.18522044865749
24	28.369408197132316	22.27759637540144	23.93685605472714	25.416139372739107
25	27.760756796122298	23.786054965914925	24.205477489523762	24.24771074843901
26	27.218514583159227	20.3812036628025	29.11304186912764	23.287239884910637
27	28.821139909981973	21.83772877592197	26.718505629111398	22.62262568498466
28	24.374186673989087	25.798029512541635	25.473743747089934	24.35404006637934
29	23.782025644392977	25.739678967538595	26.349822709853033	24.1284726782154
30	22.68433399792266	25.03977089576295	28.419923950287124	23.855971156027266
31	22.634788266615725	24.007966117883026	29.176167906304844	24.181077709196405
32	24.82345602368644	24.92344289108296	27.11576688434952	23.137334200881078
33	25.478220771003212	26.466374565728678	23.47139480187677	24.58400986139134
34	27.918944974391422	24.331580329747734	25.935101061353134	21.814373634507707
35	26.269609364740155	25.03544310598011	24.006249925382935	24.6886976038968
36	23.643834839603155	27.084726185217463	25.700281157101756	23.571157818077626
37	24.18488317952269	24.390975513663875	28.139214550924656	23.28492675588878
38	25.463894294480728	27.06055025608577	23.881564809398167	23.59399064003534
39	25.09416673630926	28.73048017573811	25.166097587182577	21.00925550077005
40	23.586976635904538	26.64687324649897	26.110227313427494	23.655922804169006
41	23.114352144792925	27.629356144267618	24.563937870846814	24.692353840092647
42	25.59917503372691	24.43470111388355	26.929298838361525	23.036825014028008
43	22.344826351165818	24.770179439118444	27.538099473501987	25.34689473621375
44	22.771113644775014	25.46128269719798	25.322196487625504	26.4454071704015
45	23.09793639044424	22.529354353458054	27.346109764687622	27.026599491410085
46	23.58988670144817	24.725483817050893	26.23625553658624	25.4483739449147
47	21.90346641038192	25.830264084717232	27.15770167500388	25.108567829896966
48	22.78409701412352	26.790809565310823	25.03312997695825	25.391963443607406
49	23.17285192392641	24.897401535320736	28.33657668843495	23.593169852317907
50	22.856550184453386	23.076297441530066	29.670580580461074	24.396571793555474
51	21.96554780864603	24.031022791036403	27.488628359260275	26.514801041057297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	575.0
1	1280.0
2	1985.0
3	4145.5
4	6306.0
5	4329.0
6	2352.0
7	2370.0
8	2388.0
9	2463.0
10	2538.0
11	2660.5
12	2783.0
13	2981.0
14	3179.0
15	3197.5
16	3216.0
17	3048.5
18	2881.0
19	2831.5
20	2782.0
21	3867.5
22	4953.0
23	4840.0
24	4727.0
25	7192.5
26	10992.0
27	12326.0
28	13603.5
29	14881.0
30	17859.5
31	20838.0
32	23006.5
33	25175.0
34	28761.5
35	32348.0
36	37151.5
37	41955.0
38	49356.0
39	56757.0
40	69279.5
41	81802.0
42	96021.0
43	110240.0
44	116810.0
45	123380.0
46	118293.0
47	113206.0
48	99739.5
49	86273.0
50	82665.0
51	79057.0
52	78616.5
53	78176.0
54	79307.5
55	80439.0
56	82164.5
57	83890.0
58	78732.0
59	73574.0
60	65141.5
61	56709.0
62	48132.0
63	39555.0
64	34606.5
65	29658.0
66	23343.0
67	17028.0
68	14804.5
69	12581.0
70	10695.5
71	8810.0
72	6808.5
73	4807.0
74	4013.0
75	2450.0
76	1681.0
77	1259.5
78	838.0
79	677.5
80	517.0
81	299.5
82	82.0
83	57.0
84	32.0
85	23.0
86	14.0
87	7.5
88	1.0
89	1.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1340176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.793379484678955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.84819299493559	12.233673497370845
2	10.797259436010991	3.6264495020692644
3	4.40849868965886	2.2210077435945346
4	2.2993815542869545	1.54457548084847
5	1.357373836856763	1.139744697245516
6	0.8499232954666345	0.8563850660184065
7	0.5953183015318291	0.6998184306258977
8	0.4106637607671687	0.5517145900134781
9	0.326454201758448	0.49340423650478094
>10	2.9490275772962713	11.867320644035468
>50	1.5102642523095737	18.744911031195585
>100	1.6324770121230843	43.10094372604091
>500	0.009366671381034091	1.0534476259183156
>1k	0.005798415616830628	1.866603728518517
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	4870	0.3633851076276549	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3716	0.27727701436229274	No Hit
ATGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	2188	0.1632621387041702	No Hit
ATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1884	0.14057855087689974	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	1703	0.1270728620718473	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	1611	0.12020809207148912	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	1512	0.11282100261458196	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	1509	0.11259715141891811	No Hit
ATATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	1409	0.10513544489678966	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11968577261494012	0.0
2	7.461706522128437E-5	0.0	0.0	0.6149192344886045	0.0
3	7.461706522128437E-5	0.0	0.0	0.8198176955862514	0.0
4	7.461706522128437E-5	0.0	0.0	1.1136596984276692	0.0
5	7.461706522128437E-5	0.0	0.0	2.2488837287042895	0.0
6	7.461706522128437E-5	0.0	0.0	2.5267576795883526	0.0
7	7.461706522128437E-5	0.0	0.0	2.96871455689402	0.0
8	1.4923413044256874E-4	0.0	0.0	4.245188691634532	0.0
9	1.4923413044256874E-4	0.0	0.0	4.405316913599408	0.0
10	1.4923413044256874E-4	0.0	0.0	4.707516027745609	0.0
11	1.4923413044256874E-4	0.0	0.0	4.983002232542591	0.0
12	1.4923413044256874E-4	0.0	0.0	5.690148159644703	0.0
13	1.4923413044256874E-4	0.0	0.0	6.269624288153198	0.0
14	1.4923413044256874E-4	0.0	0.0	6.516159071644322	0.0
15	2.238511956638531E-4	0.0	0.0	6.645918148064135	0.0
16	2.238511956638531E-4	0.0	0.0	6.7497851028521625	0.0
17	2.238511956638531E-4	0.0	0.0	6.8832750325330405	0.0
18	2.238511956638531E-4	0.0	0.0	7.17719165243968	0.0
19	2.238511956638531E-4	0.0	0.0	7.511326870500591	0.0
20	2.238511956638531E-4	0.0	0.0	7.906200379651628	0.0
21	2.9846826088513747E-4	0.0	0.0	8.160495337925765	0.0
22	2.9846826088513747E-4	0.0	0.0	8.447621804897267	0.0
23	3.7308532610642186E-4	0.0	0.0	8.870999032962835	0.0
24	4.477023913277062E-4	0.0	0.0	9.232593331025178	0.0
25	4.477023913277062E-4	0.0	0.0	9.602843208653193	0.0
26	6.715535869915593E-4	0.0	0.0	9.931979083344277	0.0
27	7.461706522128437E-4	0.0	0.0	10.208509927054356	0.0
28	7.461706522128437E-4	0.0	0.0	10.451239240219195	0.0
29	7.461706522128437E-4	0.0	0.0	10.68620837860102	0.0
30	8.207877174341281E-4	0.0	0.0	10.93371318394002	0.0
31	8.207877174341281E-4	0.0	0.0	11.186888886235838	0.0
32	8.207877174341281E-4	0.0	0.0	11.465732858967778	0.0
33	8.207877174341281E-4	0.0	0.0	11.73838361528635	0.0
34	8.207877174341281E-4	0.0	0.0	12.022525399649002	0.0
35	8.207877174341281E-4	0.0	0.0	12.300399350533064	0.0
36	8.207877174341281E-4	0.0	0.0	12.581631069352085	0.0
37	8.954047826554124E-4	0.0	0.0	12.889948782846432	0.0
38	8.954047826554124E-4	0.0	0.0	13.170882033404569	0.0
39	8.954047826554124E-4	0.0	0.0	13.465395589832978	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCTA	25	3.8911527E-5	45.000004	40
CGAACTA	25	3.8911527E-5	45.000004	18
CGAAACG	25	3.8911527E-5	45.000004	42
TCCGATA	25	3.8911527E-5	45.000004	2
GAACTAC	25	3.8911527E-5	45.000004	30
ATTCGTT	230	0.0	45.000004	1
CGTCTAG	25	3.8911527E-5	45.000004	3
CGTTAAT	25	3.8911527E-5	45.000004	24
ATCCGAT	25	3.8911527E-5	45.000004	1
TCTACGA	25	3.8911527E-5	45.000004	25
ATCCCGC	25	3.8911527E-5	45.000004	4
AACGCGC	25	3.8911527E-5	45.000004	1
TCGTAAT	25	3.8911527E-5	45.000004	2
GTGCGAC	25	3.8911527E-5	45.000004	14
CTTACCG	25	3.8911527E-5	45.000004	12
AGCGTAA	20	7.033706E-4	45.0	26
CGTATGA	20	7.033706E-4	45.0	25
AGGAACG	20	7.033706E-4	45.0	33
CCCACGT	20	7.033706E-4	45.0	22
CATTACG	20	7.033706E-4	45.0	22
>>END_MODULE