FastQCFastQC Report
Thu 26 May 2016
SRR1545685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545685_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1102809
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC34580.3135629107125531No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT32890.29823840755742836No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT32860.29796637495704154Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC27370.24818440908625158No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC24570.22279469971681407No Hit
TTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC24390.2211625041144931No Hit
ATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC21240.19259908107387588No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC20000.18135506692455355No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC15050.13646968786072655No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC12220.11080794589090225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTGA351.0195254E-746.00000415
TAACGCA351.0195254E-746.00000415
GCACAAT351.0195254E-746.00000438
CTCGATT351.0195254E-746.0000048
TAGTGCG551.8189894E-1246.0000041
TATCACG206.311342E-446.013
TCGTTTA253.4170655E-546.046
AGGTAAC253.4170655E-546.020
CGAACTA253.4170655E-546.016
CAAACGT206.311342E-446.014
ATCTAGA253.4170655E-546.013
GGTAACG253.4170655E-546.01
ACGCATC206.311342E-446.039
CTATCGC253.4170655E-546.021
CGTGCAA301.8613337E-646.034
GCGAAAG301.8613337E-646.01
ATTACGC253.4170655E-546.042
TAGCGAT206.311342E-446.020
CCAATCC253.4170655E-546.046
CAATTCG301.8613337E-646.01
Produced by FastQC (version 0.11.5)