##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545685_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1102809
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53777580705272	33.0	31.0	34.0	31.0	34.0
2	32.949047387172214	34.0	31.0	34.0	31.0	34.0
3	33.033008435730935	34.0	33.0	34.0	31.0	34.0
4	36.42077639917701	37.0	37.0	37.0	35.0	37.0
5	36.393274810053235	37.0	37.0	37.0	35.0	37.0
6	36.40652370446741	37.0	37.0	37.0	35.0	37.0
7	36.480697926839554	37.0	37.0	37.0	35.0	37.0
8	36.51618185923401	37.0	37.0	37.0	35.0	37.0
9	38.31624515215237	39.0	39.0	39.0	37.0	39.0
10	38.059667630568846	39.0	39.0	39.0	35.0	39.0
11	37.89232949676689	39.0	38.0	39.0	35.0	39.0
12	37.496057794232726	39.0	37.0	39.0	35.0	39.0
13	37.41213664378873	39.0	37.0	39.0	35.0	39.0
14	38.738787949681225	41.0	38.0	41.0	35.0	41.0
15	38.776188805133074	41.0	38.0	41.0	35.0	41.0
16	38.83810070465511	41.0	38.0	41.0	35.0	41.0
17	38.78807844332065	40.0	38.0	41.0	35.0	41.0
18	38.75284115381721	40.0	38.0	41.0	35.0	41.0
19	38.73677490843836	40.0	38.0	41.0	35.0	41.0
20	38.667757517394215	40.0	37.0	41.0	35.0	41.0
21	38.600621685169415	40.0	37.0	41.0	35.0	41.0
22	38.70331580536612	40.0	37.0	41.0	35.0	41.0
23	38.688778383201445	40.0	37.0	41.0	35.0	41.0
24	38.63498030937361	40.0	37.0	41.0	35.0	41.0
25	38.574219107751205	40.0	37.0	41.0	35.0	41.0
26	38.445553128420244	40.0	36.0	41.0	35.0	41.0
27	38.358945202659754	40.0	36.0	41.0	35.0	41.0
28	38.29783851963486	40.0	36.0	41.0	35.0	41.0
29	38.27055909046807	40.0	36.0	41.0	35.0	41.0
30	38.22426458253424	40.0	36.0	41.0	35.0	41.0
31	38.16931943790811	40.0	36.0	41.0	34.0	41.0
32	38.091204369931695	40.0	35.0	41.0	34.0	41.0
33	38.019157442494574	40.0	35.0	41.0	34.0	41.0
34	37.90324163114374	40.0	35.0	41.0	34.0	41.0
35	37.81987542720453	40.0	35.0	41.0	34.0	41.0
36	37.74832631942612	40.0	35.0	41.0	34.0	41.0
37	37.63679839391953	40.0	35.0	41.0	33.0	41.0
38	37.57750616833921	40.0	35.0	41.0	33.0	41.0
39	37.48771002050219	40.0	35.0	41.0	33.0	41.0
40	37.30374253383859	39.0	35.0	41.0	33.0	41.0
41	37.17154375780394	39.0	35.0	41.0	33.0	41.0
42	37.09695151200253	39.0	35.0	41.0	33.0	41.0
43	37.026838736354165	39.0	35.0	41.0	33.0	41.0
44	36.87689980767295	39.0	35.0	41.0	33.0	41.0
45	36.78748178515047	38.0	35.0	41.0	32.0	41.0
46	36.77354374148198	38.0	35.0	41.0	33.0	41.0
47	36.730458311457376	38.0	35.0	41.0	33.0	41.0
48	36.618130610105645	38.0	35.0	40.0	32.0	41.0
49	36.551021981140885	38.0	35.0	40.0	32.0	41.0
50	36.43721260889238	37.0	35.0	40.0	32.0	41.0
51	36.32826445921279	37.0	35.0	40.0	32.0	41.0
52	35.98075641384864	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	3.0
12	5.0
13	10.0
14	19.0
15	25.0
16	55.0
17	107.0
18	203.0
19	357.0
20	540.0
21	860.0
22	1270.0
23	1879.0
24	2552.0
25	3321.0
26	4170.0
27	5209.0
28	6650.0
29	8503.0
30	10895.0
31	14234.0
32	19036.0
33	26780.0
34	60035.0
35	128523.0
36	70240.0
37	101018.0
38	168944.0
39	466606.0
40	756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.96885045370504	13.632279025651767	46.72268724683966	11.676183273803533
2	25.01249083023443	13.38345987383128	49.721393278437155	11.882656017497137
3	25.1555799780379	14.077415037418085	45.751712218525604	15.015292766018412
4	24.06472925048671	14.676430823469886	46.91038974110657	14.348450184936828
5	23.293335473323122	17.099606550182305	44.93670254776666	14.670355428727913
6	25.73328654372607	20.421033923372043	42.96773058616678	10.87794894673511
7	87.43390741279768	2.6947549394319417	6.1190106355679	3.7523270122024757
8	88.54171483910632	2.8395669603711973	5.852690719789193	2.766027480733291
9	81.43277757073074	4.588736580858517	7.982978013418461	5.995507834992279
10	46.01386096776504	22.05821679003345	13.168191409391836	18.75973083280967
11	36.35942398003643	22.302502065180825	22.66403339109492	18.674040563687818
12	30.65762067592847	23.218254475616355	25.347000251176766	20.777124597278405
13	24.042603932321917	23.974595782225208	31.91286977164677	20.06993051380611
14	18.41606298098764	32.386297173853315	28.74677301327791	20.450866831881132
15	17.969838838819776	22.998814844637646	36.71723752707858	22.314108789463997
16	21.54806498677468	20.87324278274842	35.39660992973398	22.18208230074292
17	22.565829622355277	21.073096066499275	27.371648218322488	28.989426092822963
18	22.51133242474445	21.969443484773883	29.83481273729177	25.6844113531899
19	26.91626564527493	24.488465364355932	26.381268197847497	22.21400079252164
20	29.03712247542412	21.825447561635787	24.636995164167143	24.50043479877295
21	26.41309601209276	24.187687985861558	26.864579451201433	22.534636550844255
22	28.896209588423744	21.896901458004063	22.807394571498783	26.399494382073414
23	28.141047089749904	24.071620743029847	22.22352193353518	25.563810233685068
24	27.449993607233893	19.68881284066416	27.799555498730967	25.061638053370984
25	30.93192021465186	21.63076289729228	24.061374181748608	23.375942706307256
26	24.84455603826229	25.92461613933147	23.321354831162967	25.90947299124327
27	25.294679314369034	23.884734346564095	24.352177031562128	26.468409307504743
28	23.895162262912255	23.404506129347872	27.34127124461262	25.35906036312725
29	23.561468939771075	22.48666813564271	27.943007356668293	26.008855567917927
30	26.00142001017402	24.245993639877803	26.325864224902045	23.426722125046133
31	25.18024426713964	26.51909804871016	21.908598859820692	26.392058824329506
32	28.091809189079886	24.88391008778492	24.715884618279322	22.308396104855873
33	25.94728552269704	24.397787830893655	23.70818518891304	25.946741457496262
34	23.108988047794316	28.31097678745821	24.38527433127586	24.194760833471616
35	24.260048657564457	24.177894812247633	27.156379753883037	24.405676776304873
36	25.044137289412767	25.61032780835122	23.16484540840708	26.18068949382894
37	25.563991588751993	28.38950353143654	24.55964722812382	21.486857651687643
38	22.960004860315795	25.906117922505167	27.213869310098122	23.92000790708092
39	23.356084326479017	27.849428142135217	23.56047148690299	25.23401604448277
40	26.372472477101656	23.626756763863916	26.702538698904345	23.298232060130086
41	22.523573891761856	24.39760647582673	26.72266911133297	26.356150521078447
42	23.00851734071811	25.45708277679997	24.185239692458076	27.34916019002384
43	23.340397113190043	21.553324283715494	27.419797988590954	27.686480614503512
44	26.200094485989865	23.054762882783873	24.52591518567585	26.21922744555041
45	22.38275168229494	24.170005866836412	26.491532078537627	26.95571037233102
46	23.215715504679412	25.784065962464943	24.0952875792635	26.904930953592142
47	23.92091468241554	23.73130795994592	28.47392431508992	23.873853042548618
48	22.514415460882166	21.767685972820317	30.836346094382616	24.8815524719149
49	21.969534162307344	22.95465488584152	26.428511192781347	28.647299759069796
50	21.010981956077615	24.638083294568688	28.864019064044633	25.486915685309057
51	23.07371448727749	25.03878731493849	25.463883591809644	26.423614605974382
52	22.83876899807673	23.838488804498333	26.677421022135295	26.64532117528965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	124.0
1	93.0
2	62.0
3	53.0
4	44.0
5	68.0
6	92.0
7	135.0
8	178.0
9	285.0
10	392.0
11	508.0
12	624.0
13	710.5
14	806.5
15	816.0
16	681.5
17	547.0
18	665.0
19	783.0
20	968.0
21	1153.0
22	1449.0
23	1745.0
24	2411.5
25	3078.0
26	4021.0
27	4964.0
28	6176.0
29	7388.0
30	8231.0
31	9074.0
32	11841.0
33	14608.0
34	16894.5
35	19181.0
36	19778.0
37	20375.0
38	24942.5
39	35802.0
40	42094.0
41	51769.5
42	61445.0
43	70432.0
44	79419.0
45	91813.5
46	104208.0
47	104984.0
48	105760.0
49	95379.0
50	84998.0
51	76134.5
52	67271.0
53	66008.5
54	64746.0
55	63269.0
56	61792.0
57	63833.5
58	65875.0
59	64002.0
60	62129.0
61	58428.5
62	54728.0
63	48102.5
64	35396.5
65	29316.0
66	24414.5
67	19513.0
68	16832.0
69	14151.0
70	11889.5
71	9628.0
72	8979.5
73	8331.0
74	6566.5
75	4802.0
76	3595.5
77	2389.0
78	1844.0
79	1299.0
80	1271.0
81	1243.0
82	813.0
83	383.0
84	281.0
85	179.0
86	111.5
87	44.0
88	31.5
89	21.0
90	23.0
91	14.5
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1102809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.33320974141667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.79387691120631	12.097519071685573
2	11.771564750006478	4.080780015930588
3	4.8543174143394445	2.524227056824711
4	2.598171776252293	1.8013862536804037
5	1.5047603448116769	1.3041163333593633
6	0.9781122209524574	1.0172294565847098
7	0.6340654956053784	0.7693273157586336
8	0.5230360756900833	0.7252715201810959
9	0.4047153376194707	0.6313514249273892
>10	3.6800483500441774	15.307404086696833
>50	1.8211514731812768	23.282240331387037
>100	1.4236093483190317	33.390660595748884
>500	0.007332792816948655	0.8426785419276583
>1k	0.005237709154963325	2.225807995307089
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	3458	0.3135629107125531	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	3289	0.29823840755742836	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT	3286	0.29796637495704154	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2737	0.24818440908625158	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2457	0.22279469971681407	No Hit
TTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2439	0.2211625041144931	No Hit
ATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2124	0.19259908107387588	No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2000	0.18135506692455355	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1505	0.13646968786072655	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1222	0.11080794589090225	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3135629107125531	0.0
2	0.0	0.0	0.0	0.7757462987697779	0.0
3	0.0	0.0	0.0	2.8438288044439246	0.0
4	0.0	0.0	0.0	3.326958702730935	0.0
5	0.0	0.0	0.0	4.101888903699552	0.0
6	0.0	0.0	0.0	6.408725354979874	0.0
7	0.0	0.0	0.0	6.690460451447168	0.0
8	0.0	0.0	0.0	7.259099263789106	0.0
9	0.0	0.0	0.0	7.659440574025058	0.0
10	0.0	0.0	0.0	8.93155569096734	0.0
11	0.0	0.0	0.0	9.967999898441162	0.0
12	0.0	0.0	0.0	10.387836878371504	0.0
13	0.0	0.0	0.0	10.59403758946472	0.0
14	0.0	0.0	0.0	10.717359034973418	0.0
15	0.0	0.0	0.0	10.915217412988106	0.0
16	0.0	0.0	0.0	11.374680475041462	0.0
17	0.0	0.0	0.0	11.948397229257287	0.0
18	0.0	0.0	0.0	12.594656010242934	0.0
19	0.0	0.0	0.0	12.984660081664186	0.0
20	0.0	0.0	0.0	13.43723165117441	0.0
21	0.0	0.0	0.0	14.114320793537232	0.0
22	0.0	0.0	0.0	14.677337598804508	0.0
23	0.0	0.0	0.0	15.255044164492674	0.0
24	9.067753346227678E-5	0.0	0.0	15.743070649586647	0.0
25	9.067753346227678E-5	0.0	0.0	16.14939667703111	0.0
26	9.067753346227678E-5	0.0	0.0	16.52616182856687	0.0
27	9.067753346227678E-5	0.0	0.0	16.87663049539857	0.0
28	9.067753346227678E-5	0.0	0.0	17.22329070582485	0.0
29	9.067753346227678E-5	0.0	0.0	17.59153216921516	0.0
30	9.067753346227678E-5	0.0	0.0	17.97029222648709	0.0
31	9.067753346227678E-5	0.0	0.0	18.34034724054664	0.0
32	9.067753346227678E-5	0.0	0.0	18.718381877550872	0.0
33	9.067753346227678E-5	0.0	0.0	19.093968221151624	0.0
34	9.067753346227678E-5	0.0	0.0	19.46538339821311	0.0
35	9.067753346227678E-5	0.0	0.0	19.860012023840937	0.0
36	9.067753346227678E-5	0.0	0.0	20.239588178914026	0.0
37	9.067753346227678E-5	0.0	0.0	20.624604985994853	0.0
38	9.067753346227678E-5	0.0	0.0	21.011344666211464	0.0
39	9.067753346227678E-5	0.0	0.0	21.414315624917823	0.0
40	9.067753346227678E-5	0.0	0.0	21.820732329895748	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTGA	35	1.0195254E-7	46.000004	15
TAACGCA	35	1.0195254E-7	46.000004	15
GCACAAT	35	1.0195254E-7	46.000004	38
CTCGATT	35	1.0195254E-7	46.000004	8
TAGTGCG	55	1.8189894E-12	46.000004	1
TATCACG	20	6.311342E-4	46.0	13
TCGTTTA	25	3.4170655E-5	46.0	46
AGGTAAC	25	3.4170655E-5	46.0	20
CGAACTA	25	3.4170655E-5	46.0	16
CAAACGT	20	6.311342E-4	46.0	14
ATCTAGA	25	3.4170655E-5	46.0	13
GGTAACG	25	3.4170655E-5	46.0	1
ACGCATC	20	6.311342E-4	46.0	39
CTATCGC	25	3.4170655E-5	46.0	21
CGTGCAA	30	1.8613337E-6	46.0	34
GCGAAAG	30	1.8613337E-6	46.0	1
ATTACGC	25	3.4170655E-5	46.0	42
TAGCGAT	20	6.311342E-4	46.0	20
CCAATCC	25	3.4170655E-5	46.0	46
CAATTCG	30	1.8613337E-6	46.0	1
>>END_MODULE