##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545684_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	934677
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973598366066565	31.0	31.0	34.0	31.0	34.0
2	32.65116612476824	34.0	31.0	34.0	31.0	34.0
3	32.583418656926405	34.0	31.0	34.0	31.0	34.0
4	36.03045330097991	37.0	35.0	37.0	35.0	37.0
5	35.8044468837898	37.0	35.0	37.0	35.0	37.0
6	35.81282838884449	37.0	35.0	37.0	35.0	37.0
7	35.79155258982515	37.0	35.0	37.0	35.0	37.0
8	35.7436087546821	37.0	35.0	37.0	33.0	37.0
9	37.920536185227625	39.0	38.0	39.0	35.0	39.0
10	37.873988554334815	39.0	38.0	39.0	35.0	39.0
11	37.70867476144166	39.0	38.0	39.0	35.0	39.0
12	37.20217358509945	39.0	37.0	39.0	33.0	39.0
13	36.997804589178934	39.0	37.0	39.0	33.0	39.0
14	37.63705001834858	39.0	35.0	41.0	33.0	41.0
15	37.49080805454719	40.0	35.0	41.0	33.0	41.0
16	37.52000958619929	40.0	35.0	41.0	33.0	41.0
17	37.63872118389561	40.0	35.0	41.0	33.0	41.0
18	37.718783066235716	40.0	35.0	41.0	33.0	41.0
19	37.70214202339418	39.0	35.0	41.0	33.0	41.0
20	37.666765096391586	39.0	36.0	41.0	33.0	41.0
21	37.658202780211774	39.0	36.0	41.0	33.0	41.0
22	37.5889617482831	39.0	35.0	41.0	33.0	41.0
23	37.513997883760915	39.0	35.0	41.0	33.0	41.0
24	37.45221611315995	39.0	35.0	41.0	33.0	41.0
25	37.42116688438894	39.0	35.0	41.0	33.0	41.0
26	37.351570649539894	39.0	35.0	41.0	33.0	41.0
27	37.26366006652565	39.0	35.0	41.0	32.0	41.0
28	37.17670489377614	39.0	35.0	41.0	32.0	41.0
29	37.09305781569462	39.0	35.0	41.0	32.0	41.0
30	37.00938399040524	39.0	35.0	41.0	32.0	41.0
31	36.96801461895393	39.0	35.0	41.0	31.0	41.0
32	36.88142320823129	39.0	35.0	41.0	31.0	41.0
33	36.71586334102583	39.0	35.0	41.0	31.0	41.0
34	36.631264062344535	39.0	35.0	41.0	31.0	41.0
35	36.57994793923462	39.0	35.0	41.0	31.0	41.0
36	36.48652529162481	38.0	35.0	41.0	30.0	41.0
37	36.374525103324466	38.0	35.0	41.0	30.0	41.0
38	36.299206035881916	38.0	35.0	41.0	30.0	41.0
39	36.26463794444498	38.0	35.0	41.0	30.0	41.0
40	36.196704316036445	38.0	35.0	41.0	30.0	41.0
41	36.19176143202411	38.0	35.0	41.0	30.0	41.0
42	36.01746699662022	38.0	35.0	40.0	30.0	41.0
43	35.95067814870806	38.0	35.0	40.0	30.0	41.0
44	35.919403173502715	38.0	35.0	40.0	30.0	41.0
45	35.888498379654145	38.0	35.0	40.0	30.0	41.0
46	35.82103657199225	38.0	35.0	40.0	30.0	41.0
47	35.73391342677738	38.0	35.0	40.0	29.0	41.0
48	35.70876356217175	38.0	35.0	40.0	30.0	41.0
49	35.70990620289148	38.0	35.0	40.0	29.0	41.0
50	35.63195841986055	37.0	35.0	40.0	29.0	41.0
51	35.52233124384146	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	7.0
12	6.0
13	6.0
14	4.0
15	21.0
16	40.0
17	80.0
18	177.0
19	418.0
20	736.0
21	1239.0
22	1908.0
23	2783.0
24	4328.0
25	6049.0
26	8524.0
27	10361.0
28	11959.0
29	14137.0
30	17553.0
31	22148.0
32	28162.0
33	36658.0
34	59482.0
35	124935.0
36	64501.0
37	86351.0
38	130716.0
39	301213.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.7328981027670523	91.58029993249005	2.931814947837595	3.7549870169053055
2	3.549889427042711	2.805996082068993	90.54347116704487	3.100643323843424
3	26.419929023609225	15.537560034108038	44.217093177643186	13.82541776463955
4	26.716716042012372	16.05228330214609	43.88200415758599	13.348996498255547
5	23.379734389527076	14.710536367108636	47.00618502434531	14.903544219018977
6	19.931591341179892	15.489629037624763	48.93658450994301	15.642195111252336
7	23.272638569259755	17.12334849365075	45.59457438238022	14.009438554709275
8	23.755158199035602	19.372146741601647	43.685465674238266	13.187229385124489
9	79.87058630949515	4.641068518857317	10.611901223631264	4.8764439480162665
10	81.03152211940595	4.552909721754146	10.634047911738493	3.7815202471014056
11	74.06911692488421	7.2486003186127395	11.351515015347548	7.330767741155501
12	38.942008843696804	27.14873694335048	17.90404599663841	16.005208216314298
13	32.79710531017667	24.66606110988074	27.433434223801378	15.103399356141212
14	30.60511813171823	25.078931010391827	26.220929797138474	18.095021060751467
15	20.645741791014434	33.00894319641973	30.058405203080852	16.286909809484985
16	13.778128701144887	38.40567383170871	28.781814466387857	19.034383000758552
17	13.548423680052037	27.76231789163529	41.33246030446882	17.356798123843852
18	16.677419044225974	22.714049880332993	40.69416493612232	19.914366139318716
19	16.36255091331016	23.54588804474701	30.43094031414061	29.660620727802222
20	18.886203469219847	24.64765903087377	34.18592733104591	22.280210168860474
21	24.20172958144899	26.728377824638887	29.44279146699876	19.627101126913363
22	25.537164175431727	24.262820204198885	27.967950425655065	22.232065194714323
23	20.46386077757343	29.13819426390079	29.46985964135204	20.92808531717374
24	24.916842930766457	24.631503717326947	25.924463745229637	24.52718960667696
25	22.26929730805401	29.453062394816605	23.72252660544766	24.555113691681726
26	20.692816876846226	22.808734996153753	35.60192451509987	20.896523611900154
27	22.58480737195844	26.259124809961087	30.67851247008325	20.477555347997225
28	18.980246652051992	31.941515625183886	27.550266027729364	21.527971695034758
29	18.93017587894	30.189787488084118	28.943260613024606	21.936776019951274
30	18.478148066123374	29.51661376068952	32.50941234244557	19.495825830741527
31	17.169888635325357	27.897123819244506	30.83375326449672	24.099234280933413
32	19.5881571922707	29.936330946412504	30.456296667190912	20.019215194125888
33	20.949590072292352	29.60252579233254	28.000475030411575	21.447409104963533
34	23.41792940234969	26.77684376527934	27.228764589264525	22.576462243106445
35	23.803731128507494	26.847563382858464	26.74132347324263	22.607382015391416
36	18.919049040470664	25.24080511235432	32.33994203345113	23.500203813723886
37	18.008574084951274	25.553319488978545	32.66133648308453	23.776769942985652
38	21.992196234635067	28.701786820473814	26.26276243022991	23.043254514661214
39	20.714107654301966	30.716493505242987	29.721069417563502	18.848329422891545
40	19.82139284480093	27.269313356378728	30.919130351982556	21.990163446837784
41	19.773675825980526	29.446536076099015	26.473851394652915	24.305936703267548
42	23.276811133685754	23.913180703066406	29.126318503611408	23.683689659636432
43	18.30899872362324	24.369381080309026	30.725908522409345	26.595711673658386
44	18.680570935200073	26.995849903228603	27.570379928039312	26.75319923353201
45	19.535197720709935	26.568429521642233	28.48288767135599	25.413485086291843
46	22.57111280153465	26.28405320768565	28.80235632202354	22.342477668756157
47	17.478979369343634	29.16429953877115	28.731422726781553	24.625298365103667
48	16.99667371723066	29.5171487048467	27.575515391948237	25.910662185974402
49	19.462659292996403	24.947120770062813	31.423475703371324	24.166744233569457
50	19.285699765801446	22.758985189536066	34.17801015751966	23.77730488714283
51	17.59121065351988	23.85326695746231	31.03917182085362	27.51635056816419
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1125.0
1	1036.5
2	948.0
3	2831.0
4	4714.0
5	3244.0
6	1774.0
7	1997.5
8	2221.0
9	2431.0
10	2641.0
11	2949.5
12	3258.0
13	3134.0
14	3010.0
15	3256.5
16	3503.0
17	3255.5
18	3008.0
19	3276.0
20	3544.0
21	4016.0
22	4488.0
23	5065.5
24	5643.0
25	7024.0
26	11033.0
27	13661.0
28	15460.0
29	17259.0
30	20888.0
31	24517.0
32	25294.5
33	26072.0
34	31345.5
35	36619.0
36	41203.0
37	45787.0
38	54065.5
39	62344.0
40	72427.0
41	82510.0
42	91935.5
43	101361.0
44	108983.0
45	116605.0
46	107853.5
47	99102.0
48	81401.0
49	63700.0
50	53686.0
51	43672.0
52	40191.5
53	36711.0
54	32681.5
55	28652.0
56	27814.0
57	26976.0
58	25278.5
59	23581.0
60	19116.0
61	14651.0
62	11486.5
63	8322.0
64	6999.0
65	5676.0
66	4183.5
67	2691.0
68	2620.0
69	2549.0
70	2211.5
71	1874.0
72	1354.5
73	835.0
74	598.5
75	271.0
76	180.0
77	140.0
78	100.0
79	56.0
80	12.0
81	11.5
82	11.0
83	6.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	934677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.18054016382966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.10774812790076	12.745967306349534
2	11.894538755150442	4.324982791364822
3	5.412892977512838	2.952279545405511
4	2.8110748283089864	2.044274352784084
5	1.7491120196024383	1.5899900661709674
6	1.1028142070599223	1.2029854790816927
7	0.7887214046513167	1.0037566822734787
8	0.547102817042653	0.7957299791190644
9	0.45915674342764495	0.7512945853841577
>10	2.737829593370876	10.346564048419152
>50	0.8642728668390708	11.640684273536172
>100	1.5022870132411188	44.96566579997319
>500	0.01240583062448427	1.5979305120836205
>1k	0.00945206142817849	3.2663168929618944
>5k	5.907538392611556E-4	0.771577685092669
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	7184	0.768607765035408	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3024	0.32353422626212053	No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	2791	0.29860582853755896	No Hit
ATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	2775	0.2968940072345848	No Hit
ATGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT	2528	0.27046776586992083	No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	2227	0.23826412760771903	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2222	0.2377291834505396	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	2126	0.22745825563269448	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	2027	0.21686636132054174	No Hit
ATATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	1666	0.17824339317218676	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	1649	0.17642458303777667	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	1585	0.16957729782587996	No Hit
ATTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	1440	0.15406391726767643	No Hit
ATACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	1198	0.12817262006019192	No Hit
ATAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	1095	0.1171527704222956	No Hit
ATTTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1042	0.11148236235619363	No Hit
ATTGTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1017	0.10880764157029647	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3313444109569402	0.0
2	0.0	0.0	0.0	1.4686356891204126	0.0
3	0.0	0.0	0.0	1.9358559160009288	0.0
4	0.0	0.0	0.0	2.5406637801079945	0.0
5	0.0	0.0	0.0	4.69220918028367	0.0
6	0.0	0.0	0.0	5.201048062592746	0.0
7	0.0	0.0	0.0	6.000040655755946	0.0
8	1.069888314358864E-4	0.0	0.0	8.292276369269812	0.0
9	1.069888314358864E-4	0.0	0.0	8.595268739896243	0.0
10	1.069888314358864E-4	0.0	0.0	9.189805676185463	0.0
11	1.069888314358864E-4	0.0	0.0	9.661091478660543	0.0
12	2.139776628717728E-4	0.0	0.0	10.847811596947395	0.0
13	2.139776628717728E-4	0.0	0.0	11.8635635625997	0.0
14	2.139776628717728E-4	0.0	0.0	12.321154794650987	0.0
15	2.139776628717728E-4	0.0	0.0	12.549896916260911	0.0
16	2.139776628717728E-4	0.0	0.0	12.705779643662998	0.0
17	2.139776628717728E-4	0.0	0.0	12.956775442211587	0.0
18	3.209664943076592E-4	0.0	0.0	13.456520273848612	0.0
19	3.209664943076592E-4	0.0	0.0	14.167568047571514	0.0
20	3.209664943076592E-4	0.0	0.0	15.000048144974146	0.0
21	3.209664943076592E-4	0.0	0.0	15.43795343204123	0.0
22	3.209664943076592E-4	0.0	0.0	15.944331571227279	0.0
23	5.34944157179432E-4	0.0	0.0	16.612904778870135	0.0
24	5.34944157179432E-4	0.0	0.0	17.22627174949207	0.0
25	6.419329886153184E-4	0.0	0.0	17.83642905517093	0.0
26	6.419329886153184E-4	0.0	0.0	18.274869286395194	0.0
27	9.628994829229777E-4	0.0	0.0	18.704429444610277	0.0
28	0.0011768771457947504	0.0	0.0	19.079211321130188	0.0
29	0.0011768771457947504	0.0	0.0	19.465654980276607	0.0
30	0.0011768771457947504	0.0	0.0	19.832519683270263	0.0
31	0.0011768771457947504	0.0	0.0	20.22217300735976	0.0
32	0.0011768771457947504	0.0	0.0	20.68650453579151	0.0
33	0.0011768771457947504	0.0	0.0	21.09113629628203	0.0
34	0.0012838659772306368	0.0	0.0	21.52133838748573	0.0
35	0.0012838659772306368	0.0	0.0	21.933887321502507	0.0
36	0.0013908548086665232	0.0	0.0	22.306636410225135	0.0
37	0.0013908548086665232	0.0	0.0	22.768507195533857	0.0
38	0.001604832471538296	0.0	0.0	23.206091516106632	0.0
39	0.001604832471538296	0.0	0.0	23.623561936369462	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAACGA	35	1.2116107E-7	45.000004	11
TACGGTA	35	1.2116107E-7	45.000004	25
AGAACCG	35	1.2116107E-7	45.000004	30
GTAATAC	35	1.2116107E-7	45.000004	24
GTTACCG	35	1.2116107E-7	45.000004	18
TACGCTA	35	1.2116107E-7	45.000004	2
CCCGTAT	35	1.2116107E-7	45.000004	41
TGTTACG	35	1.2116107E-7	45.000004	3
CTCGCTA	35	1.2116107E-7	45.000004	42
TCTCCGA	35	1.2116107E-7	45.000004	24
ATGCGTC	70	0.0	45.000004	1
GCGTGCA	35	1.2116107E-7	45.000004	11
CGGTATG	70	0.0	45.000004	3
CGACTGA	35	1.2116107E-7	45.000004	25
CGGTAAC	35	1.2116107E-7	45.000004	27
CGTCCGG	70	0.0	45.000004	4
CATCAGG	35	1.2116107E-7	45.000004	20
CGATAGG	35	1.2116107E-7	45.000004	4
TAGTCAG	35	1.2116107E-7	45.000004	34
TCGCGGT	35	1.2116107E-7	45.000004	13
>>END_MODULE