FastQCFastQC Report
Thu 26 May 2016
SRR1545683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545683_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences764288
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT47490.6213626277005527Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC35990.47089578797521353No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT35650.4664472031485513No Hit
TTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC33330.4360921537430916No Hit
GCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC32370.4235314436442807No Hit
GTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC31630.41384922960978054No Hit
GGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC23900.3127093451683135No Hit
ATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC23780.3111392564059622No Hit
ACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC14860.19442932507117736No Hit
TCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC14600.19102746608608273No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC13510.1767658264947245No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT12660.165644364428069No Hit
CTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC11360.1486350695025959No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT10100.13214913749790655No Hit
AACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC10040.13136409311673086No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC9160.11985010885948752No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT9000.11775665717635238No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCA206.310121E-446.020
TCACGCA301.8605788E-646.012
TCGCCTC206.310121E-446.042
GTTGACG600.046.01
ACAACCG253.4160734E-546.030
TAGAGTA206.310121E-446.014
CTATGCG301.8605788E-646.01
TCGTTAG600.046.01
GGTACGC253.4160734E-546.032
GTACCGA206.310121E-446.023
ATCTCCG301.8605788E-646.044
GTCGCAT453.092282E-1046.015
GTCGCAA301.8605788E-646.027
CAAACGA206.310121E-446.029
ACACGGA253.4160734E-546.019
ACACGAC1000.046.026
ACGCCGT206.310121E-446.034
ACGCCAT206.310121E-446.031
GTCGATT405.6079443E-946.010
CGTATGT253.4160734E-546.010
Produced by FastQC (version 0.11.5)