##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545683_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	764288
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60286174845085	33.0	31.0	34.0	31.0	34.0
2	32.98292135948752	34.0	31.0	34.0	31.0	34.0
3	33.0472099522693	34.0	33.0	34.0	31.0	34.0
4	36.419887529308326	37.0	37.0	37.0	35.0	37.0
5	36.39956011346508	37.0	37.0	37.0	35.0	37.0
6	36.39874235890136	37.0	37.0	37.0	35.0	37.0
7	36.48240846382515	37.0	37.0	37.0	35.0	37.0
8	36.53958193769888	37.0	37.0	37.0	35.0	37.0
9	38.335887780522526	39.0	39.0	39.0	37.0	39.0
10	38.091655239909564	39.0	39.0	39.0	35.0	39.0
11	37.93611701348183	39.0	38.0	39.0	35.0	39.0
12	37.53898268715458	39.0	37.0	39.0	35.0	39.0
13	37.44688258876235	39.0	37.0	39.0	35.0	39.0
14	38.761626507285214	41.0	38.0	41.0	35.0	41.0
15	38.80562562803551	41.0	38.0	41.0	35.0	41.0
16	38.87435233838553	41.0	38.0	41.0	35.0	41.0
17	38.850196522776756	41.0	38.0	41.0	35.0	41.0
18	38.85822490998158	41.0	38.0	41.0	35.0	41.0
19	38.826552294423045	40.0	37.0	41.0	35.0	41.0
20	38.7546095189248	40.0	37.0	41.0	35.0	41.0
21	38.71089693937364	40.0	37.0	41.0	35.0	41.0
22	38.773103856138	40.0	37.0	41.0	35.0	41.0
23	38.788443623346176	41.0	37.0	41.0	35.0	41.0
24	38.77986308825992	41.0	37.0	41.0	35.0	41.0
25	38.71529318790822	40.0	37.0	41.0	35.0	41.0
26	38.62106692765031	40.0	37.0	41.0	35.0	41.0
27	38.53674531066823	40.0	36.0	41.0	35.0	41.0
28	38.51487135739407	40.0	36.0	41.0	35.0	41.0
29	38.471862700971364	40.0	36.0	41.0	35.0	41.0
30	38.432962443476804	40.0	36.0	41.0	35.0	41.0
31	38.290472963071515	40.0	36.0	41.0	35.0	41.0
32	38.23014230237816	40.0	36.0	41.0	35.0	41.0
33	38.197591745520015	40.0	36.0	41.0	35.0	41.0
34	38.09642700133981	40.0	36.0	41.0	34.0	41.0
35	38.05760524828337	40.0	36.0	41.0	34.0	41.0
36	37.969909248869534	40.0	35.0	41.0	34.0	41.0
37	37.91701295846592	40.0	35.0	41.0	34.0	41.0
38	37.844977286049236	40.0	35.0	41.0	34.0	41.0
39	37.82689509713616	40.0	35.0	41.0	34.0	41.0
40	37.685351595210186	40.0	35.0	41.0	34.0	41.0
41	37.61193293627533	40.0	35.0	41.0	34.0	41.0
42	37.60831519008541	40.0	35.0	41.0	34.0	41.0
43	37.52500628035505	40.0	35.0	41.0	33.0	41.0
44	37.40657710182549	40.0	35.0	41.0	33.0	41.0
45	37.329104997069166	39.0	35.0	41.0	33.0	41.0
46	37.361902842907384	39.0	35.0	41.0	33.0	41.0
47	37.37077515282197	39.0	35.0	41.0	33.0	41.0
48	37.31649587590019	39.0	35.0	41.0	34.0	41.0
49	37.31428990118908	39.0	35.0	41.0	34.0	41.0
50	37.22885613800033	39.0	35.0	41.0	33.0	41.0
51	37.140299206581815	39.0	35.0	41.0	33.0	41.0
52	36.770129846340645	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	5.0
13	10.0
14	15.0
15	14.0
16	12.0
17	31.0
18	64.0
19	140.0
20	247.0
21	410.0
22	642.0
23	1103.0
24	1713.0
25	2292.0
26	2627.0
27	3231.0
28	3948.0
29	5117.0
30	6511.0
31	8461.0
32	11179.0
33	16347.0
34	37420.0
35	96058.0
36	39589.0
37	52334.0
38	95577.0
39	378644.0
40	543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.145526293753143	13.983603039691845	46.08223601574276	12.78863465081226
2	24.98521499748786	13.859173505275498	48.43841588511137	12.717195612125273
3	24.99319628202981	14.602741374979065	44.92638900519176	15.477673337799363
4	20.515041450343325	15.344739155920282	48.476228856138	15.663990537598394
5	23.59921914252219	17.188939248032156	44.591436735890134	14.620404873555518
6	22.676006950259588	19.013644071344835	44.52549300787138	13.784855970524202
7	82.55395871713282	3.286064938871211	8.190368028805896	5.969608315190086
8	84.60227034835036	2.9751873639256408	8.571637916596885	3.8509043711271147
9	76.11829572098476	4.602191843912243	10.932658892982749	8.346853542120247
10	39.14152780103835	26.348444565399433	16.02498011220901	18.485047521353206
11	32.198856975381005	22.624717384022777	29.253370457209847	15.923055183386367
12	30.50590876737565	25.511848936526544	25.532260090437113	18.449982205660692
13	21.06758185396081	30.924991626193265	32.52870645620499	15.47872006364093
14	13.838762351364931	39.00479923798359	28.482718556355717	18.67371985429576
15	13.437997194774745	27.10130212694691	40.425729567911574	19.034971110366772
16	16.427707879752134	24.169161363255736	38.47489428069	20.928236476302125
17	15.763952855468096	24.730468095796347	28.494232540612963	31.011346508122596
18	18.29532846256908	25.33416722492045	32.13971696533244	24.230787347178026
19	22.60064268966672	27.925075364260593	29.520808909730363	19.95347303634232
20	25.114093116730867	22.884043711271143	26.894835454697706	25.107027717300284
21	19.701473789984927	30.342357854630713	29.92353667727349	20.032631678110867
22	24.49809495896835	24.175180036844747	25.615082272651147	25.711642731535754
23	22.480138377156255	29.89985450510802	22.238213867023948	25.381793250711777
24	21.174871252721488	21.68292580807235	33.70404873555518	23.438154203650978
25	25.09629877742422	24.884075113046393	28.19447642773405	21.825149681795345
26	18.218132431753475	31.32392501256071	26.873639256405962	23.584303299279853
27	17.738470314855135	29.08811861497237	28.56501737564897	24.60839369452353
28	19.117269929660026	30.01604107352202	30.4751611957796	20.391527801038354
29	17.460433763188746	28.211224041199127	29.752920365097975	24.575421830514152
30	21.007918480991457	29.446883897169652	30.150545344163454	19.39465227767543
31	20.009734550326577	30.965944774744596	26.119604128286717	22.904716546642103
32	23.300771436945237	27.57272127784291	24.4834407971864	24.643066488025454
33	24.058470105509965	25.875324485010886	26.217211313012896	23.848994096466253
34	16.74617421704907	24.2897965164964	34.35642061631218	24.607608650142353
35	17.099575029308323	24.34108608273321	32.167324359403786	26.39201452855468
36	21.062871587673758	26.914592404957293	25.914053341148886	26.108482666220063
37	22.18195235303969	29.138884818288396	29.221576159772233	19.457586668899683
38	18.672934809914587	25.630259797353876	33.17388209680121	22.52292329593033
39	19.823679031987943	28.69690483168649	26.384033244012727	25.095382892312845
40	23.717237481158936	22.04836396750963	29.85353688661866	24.38086166471278
41	17.975684558700387	23.385948752302795	31.091944397923292	27.546422291073526
42	18.800635362585833	26.888947621838888	26.76491060961313	27.54550640596215
43	19.47237167141182	25.096822140344997	29.15615579467426	26.274650393568916
44	25.93394113213867	24.574113423212193	26.093828504438115	23.39811694021102
45	17.98288079886116	26.79997592530564	28.35541052587506	26.86173274995813
46	17.239705451348183	27.810328043878744	26.72238737229945	28.227579132473622
47	19.79973517836208	23.7280972617652	31.947773614134984	24.52439394573773
48	18.381159981577625	20.737601532406632	36.57103081560877	24.310207670406967
49	17.828750418690337	21.638832481996314	30.269217886451184	30.26319921286217
50	17.20071491374979	23.999330095461396	33.73152528889633	25.06842970189248
51	20.53244326745939	23.247780941215876	29.126847471110366	27.09292832021437
52	18.424075741081896	21.490066571763524	31.943325029308323	28.14253265784626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	617.0
1	459.5
2	302.0
3	263.0
4	224.0
5	270.5
6	317.0
7	460.0
8	603.0
9	727.5
10	852.0
11	1134.0
12	1416.0
13	1302.0
14	1313.5
15	1439.0
16	1294.0
17	1149.0
18	1193.5
19	1238.0
20	1804.0
21	2370.0
22	2343.5
23	2317.0
24	2640.5
25	2964.0
26	3748.0
27	4532.0
28	5859.0
29	7186.0
30	9819.0
31	12452.0
32	14083.5
33	15715.0
34	17968.5
35	20222.0
36	22233.0
37	24244.0
38	27773.0
39	38626.5
40	45951.0
41	54684.0
42	63417.0
43	70265.0
44	77113.0
45	88459.5
46	99806.0
47	99172.5
48	98539.0
49	82508.0
50	66477.0
51	54251.0
52	42025.0
53	37110.0
54	32195.0
55	29063.5
56	25932.0
57	24277.0
58	22622.0
59	20553.5
60	18485.0
61	15954.0
62	13423.0
63	11538.5
64	7941.5
65	6229.0
66	4725.5
67	3222.0
68	2706.5
69	2191.0
70	1878.5
71	1566.0
72	1349.5
73	1133.0
74	942.5
75	752.0
76	643.5
77	535.0
78	372.0
79	209.0
80	136.5
81	64.0
82	65.5
83	67.0
84	39.5
85	12.0
86	11.0
87	10.0
88	6.0
89	3.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	764288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.0681676317839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.52308119299562	12.494050959390275
2	12.966596332400831	4.944984649597868
3	6.133731750941813	3.508770757060616
4	3.4016953433735932	2.594563881588255
5	2.173545646211429	2.0722766368646797
6	1.5155037478115176	1.7338727705920063
7	0.9836780453821038	1.3129856505536042
8	0.7331582465337173	1.1183987476423736
9	0.5381553842758541	0.923547337138716
>10	3.5770814794297037	13.959313110103388
>50	1.1870435194156057	16.774074950506325
>100	1.2502425956807883	33.13252463888177
>500	0.006182518330289613	0.8294166907698547
>1k	0.010304197217149355	4.601219219310279
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT	4749	0.6213626277005527	Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	3599	0.47089578797521353	No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	3565	0.4664472031485513	No Hit
TTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	3333	0.4360921537430916	No Hit
GCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	3237	0.4235314436442807	No Hit
GTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	3163	0.41384922960978054	No Hit
GGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	2390	0.3127093451683135	No Hit
ATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	2378	0.3111392564059622	No Hit
ACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1486	0.19442932507117736	No Hit
TCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1460	0.19102746608608273	No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1351	0.1767658264947245	No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	1266	0.165644364428069	No Hit
CTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1136	0.1486350695025959	No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	1010	0.13214913749790655	No Hit
AACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1004	0.13136409311673086	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	916	0.11985010885948752	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	900	0.11775665717635238	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6538111287891476	0.0
2	0.0	0.0	0.0	1.5147431334784793	0.0
3	0.0	0.0	0.0	5.2409562887288565	0.0
4	0.0	0.0	0.0	6.0640753223915596	0.0
5	0.0	0.0	0.0	7.434893652654496	0.0
6	0.0	0.0	0.0	11.344807193099983	0.0
7	0.0	0.0	0.0	11.85953462569084	0.0
8	0.0	0.0	0.0	12.860858733880422	0.0
9	0.0	0.0	0.0	13.488240035169989	0.0
10	0.0	0.0	0.0	15.525168522860492	0.0
11	0.0	0.0	0.0	17.213537305308993	0.0
12	0.0	0.0	0.0	17.95972198961648	0.0
13	0.0	0.0	0.0	18.325029308323565	0.0
14	0.0	0.0	0.0	18.513570800535923	0.0
15	0.0	0.0	0.0	18.844728688661867	0.0
16	0.0	0.0	0.0	19.59039001004857	0.0
17	0.0	0.0	0.0	20.691283913917268	0.0
18	0.0	0.0	0.0	22.00348559705242	0.0
19	0.0	0.0	0.0	22.693277926645454	0.0
20	0.0	0.0	0.0	23.443126151398427	0.0
21	3.925221905878412E-4	0.0	0.0	24.477160442136995	0.0
22	3.925221905878412E-4	0.0	0.0	25.3582419192765	0.0
23	3.925221905878412E-4	0.0	0.0	26.28315504103165	0.0
24	3.925221905878412E-4	0.0	0.0	26.92859236308826	0.0
25	6.542036509797354E-4	0.0	0.0	27.52156255233629	0.0
26	7.850443811756824E-4	0.0	0.0	28.063504856807906	0.0
27	7.850443811756824E-4	0.0	0.0	28.616568623346172	0.0
28	7.850443811756824E-4	0.0	0.0	29.10735220231117	0.0
29	7.850443811756824E-4	0.0	0.0	29.620771227600066	0.0
30	7.850443811756824E-4	0.0	0.0	30.226040445486518	0.0
31	7.850443811756824E-4	0.0	0.0	30.779889256405962	0.0
32	7.850443811756824E-4	0.0	0.0	31.34237355551834	0.0
33	7.850443811756824E-4	0.0	0.0	31.84754961480489	0.0
34	7.850443811756824E-4	0.0	0.0	32.32603416513147	0.0
35	7.850443811756824E-4	0.0	0.0	32.912462317869704	0.0
36	9.158851113716295E-4	0.0	0.0	33.45558218891308	0.0
37	9.158851113716295E-4	0.0	0.0	33.996870289733714	0.0
38	9.158851113716295E-4	0.0	0.0	34.51604630715123	0.0
39	0.0011775665717635238	0.0	0.0	35.0174279852621	0.0
40	0.0011775665717635238	0.0	0.0	35.53359466588511	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGCA	20	6.310121E-4	46.0	20
TCACGCA	30	1.8605788E-6	46.0	12
TCGCCTC	20	6.310121E-4	46.0	42
GTTGACG	60	0.0	46.0	1
ACAACCG	25	3.4160734E-5	46.0	30
TAGAGTA	20	6.310121E-4	46.0	14
CTATGCG	30	1.8605788E-6	46.0	1
TCGTTAG	60	0.0	46.0	1
GGTACGC	25	3.4160734E-5	46.0	32
GTACCGA	20	6.310121E-4	46.0	23
ATCTCCG	30	1.8605788E-6	46.0	44
GTCGCAT	45	3.092282E-10	46.0	15
GTCGCAA	30	1.8605788E-6	46.0	27
CAAACGA	20	6.310121E-4	46.0	29
ACACGGA	25	3.4160734E-5	46.0	19
ACACGAC	100	0.0	46.0	26
ACGCCGT	20	6.310121E-4	46.0	34
ACGCCAT	20	6.310121E-4	46.0	31
GTCGATT	40	5.6079443E-9	46.0	10
CGTATGT	25	3.4160734E-5	46.0	10
>>END_MODULE