FastQCFastQC Report
Thu 26 May 2016
SRR1545682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545682_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2647909
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC68470.25858139384699397No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58880.22236413713613268No Hit
ATGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA44120.1666220402589364No Hit
ATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT36060.13618292773656498No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA33710.12730800038823084No Hit
ATGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC30690.11590277460441427No Hit
ATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG29600.11178631894071889No Hit
ATGTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC28160.10634806558684608No Hit
ATGGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTG27870.10525286178641335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG351.2127384E-745.03
CGTCGGT207.0349546E-445.036
CCGCGTA253.89219E-545.036
CGTCCTA207.0349546E-445.043
ATCGCTA1300.043.2692341
ATTACGC1400.041.7857131
CGCGTAA950.040.26315733
CGTAATC403.4605E-739.37545
TCGTTGC1800.038.752
CGAATCC356.2513755E-638.57142639
CGTTTTT31800.038.4905621
TTCGCGA659.094947E-1238.0769272
ATCTCGT2100.037.5000041
CGGTAAG601.5643309E-1037.4999963
ATGTTTT51450.037.4781341
ATTAGTT16550.036.5709951
AACGTCC250.002107604236.013
ACGATTG504.883077E-836.03
TTACGCG504.883077E-836.03
TAGGCGT250.002107604236.036