##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545682_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2647909
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847695672321066	31.0	31.0	34.0	31.0	34.0
2	32.508701016537955	33.0	31.0	34.0	31.0	34.0
3	32.502234404581124	34.0	31.0	34.0	31.0	34.0
4	35.98514941412262	37.0	35.0	37.0	35.0	37.0
5	35.761806391382784	37.0	35.0	37.0	35.0	37.0
6	35.77363950196174	37.0	35.0	37.0	35.0	37.0
7	35.74315922488273	37.0	35.0	37.0	33.0	37.0
8	35.70367712787713	37.0	35.0	37.0	33.0	37.0
9	37.851653134605456	39.0	38.0	39.0	35.0	39.0
10	37.79744734430073	39.0	38.0	39.0	35.0	39.0
11	37.633442085811865	39.0	37.0	39.0	35.0	39.0
12	37.18103039039484	39.0	37.0	39.0	33.0	39.0
13	36.95357959809042	39.0	37.0	39.0	33.0	39.0
14	37.69591062230613	40.0	36.0	41.0	33.0	41.0
15	37.61297008318639	40.0	36.0	41.0	33.0	41.0
16	37.44525359443999	40.0	36.0	41.0	32.0	41.0
17	37.56245701797154	40.0	36.0	41.0	32.0	41.0
18	37.65220217160031	40.0	36.0	41.0	33.0	41.0
19	37.64296771528024	40.0	36.0	41.0	33.0	41.0
20	37.60371258982087	40.0	35.0	41.0	32.0	41.0
21	37.59382289950297	40.0	35.0	41.0	32.0	41.0
22	37.498369468135046	40.0	35.0	41.0	32.0	41.0
23	37.45352276078974	40.0	35.0	41.0	32.0	41.0
24	37.473295721265345	40.0	35.0	41.0	32.0	41.0
25	37.42047593025289	39.0	35.0	41.0	32.0	41.0
26	37.33033537028652	39.0	35.0	41.0	32.0	41.0
27	37.241153680130246	39.0	35.0	41.0	32.0	41.0
28	37.15685546595446	39.0	35.0	41.0	32.0	41.0
29	37.105031177430945	39.0	35.0	41.0	31.0	41.0
30	37.02941150923238	39.0	35.0	41.0	31.0	41.0
31	36.906547392678526	39.0	35.0	41.0	31.0	41.0
32	36.776681902588045	39.0	35.0	41.0	31.0	41.0
33	36.65295899519206	39.0	35.0	41.0	30.0	41.0
34	36.597319243221726	39.0	35.0	41.0	30.0	41.0
35	36.50792644309151	39.0	35.0	41.0	30.0	41.0
36	36.379907315545964	39.0	35.0	41.0	30.0	41.0
37	36.25163817940874	38.0	35.0	41.0	30.0	41.0
38	36.19312144035161	38.0	35.0	41.0	30.0	41.0
39	36.13441700602249	38.0	35.0	41.0	30.0	41.0
40	36.033648814970604	38.0	35.0	40.0	30.0	41.0
41	35.95468008908161	38.0	35.0	40.0	29.0	41.0
42	35.81782191155361	38.0	35.0	40.0	29.0	41.0
43	35.6994443540167	38.0	35.0	40.0	29.0	41.0
44	35.63102130775642	38.0	34.0	40.0	29.0	41.0
45	35.61152592479576	38.0	34.0	40.0	29.0	41.0
46	35.56480075410447	37.0	34.0	40.0	29.0	41.0
47	35.4919342016663	37.0	34.0	40.0	29.0	41.0
48	35.43094683389799	37.0	34.0	40.0	29.0	41.0
49	35.3904526175182	37.0	34.0	40.0	28.0	41.0
50	35.278663654982104	37.0	34.0	40.0	28.0	41.0
51	35.07306557740466	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	16.0
10	24.0
11	26.0
12	63.0
13	55.0
14	77.0
15	109.0
16	211.0
17	425.0
18	856.0
19	1589.0
20	2808.0
21	4520.0
22	6691.0
23	9744.0
24	13654.0
25	19121.0
26	25445.0
27	31293.0
28	36862.0
29	44255.0
30	54316.0
31	66636.0
32	83146.0
33	108114.0
34	176447.0
35	264794.0
36	199174.0
37	280425.0
38	426014.0
39	790650.0
40	345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.3223230103451438	90.88669587965448	3.5061250216680406	3.2848560883323406
2	3.4943421394013163	2.8404677048946922	90.49918256254274	3.1660075931612455
3	30.157569614363634	15.775088947543136	40.86843618870588	13.198905249387346
4	27.63761896651282	15.951945478488874	42.177053667629814	14.233381887368484
5	25.49706202139122	14.474477786056847	44.92450458078431	15.103955611767624
6	24.172167548053956	14.460089074058057	45.92480330706229	15.442940070825697
7	23.835146902707006	16.922333811320556	43.806603625728826	15.43591566024361
8	26.444639902655265	19.420493680107587	42.324301930315585	11.810564486921567
9	81.65031351152928	4.439691847416206	9.581144971371751	4.328849669682757
10	82.11422673513327	4.39456189770872	9.361953148692043	4.129258218465967
11	76.01545219265465	7.02762066219043	10.513729890264356	6.443197254890556
12	43.8235226361631	21.569585661742906	17.312679552054092	17.2942121500399
13	33.938590789940285	23.904031445189393	23.725324397477408	18.43205336739291
14	29.239713298304437	22.165263232233436	28.406791925251206	20.18823154421092
15	25.099993995261922	23.267227083710203	32.41089478528152	19.221884135746357
16	20.167045015519793	27.156069185157044	33.223573770850884	19.45331202847228
17	20.296958845640088	22.204879397290465	37.20275130300928	20.295410454060168
18	25.949154597080188	20.715855416481457	32.50058819997213	20.834401786466227
19	25.587284155157903	21.37747180888769	28.763450707709364	24.271793328245042
20	25.849453285592517	21.434497937806775	30.878062652455203	21.837986124145505
21	27.35713349665717	24.047238783508043	27.60838835473576	20.98723936509903
22	25.880496648487544	25.21884249043302	27.070983179557906	21.829677681521535
23	26.9956029455695	23.627322540162822	29.238995750986913	20.138078763280763
24	28.191678792586906	21.14891410543187	27.962252479220396	22.697154622760827
25	27.54894522432606	22.733938364196053	26.72448335648997	22.99263305498792
26	26.75771712698586	22.526907080265975	28.09552745203857	22.619848340709595
27	28.765867709199977	22.879185047522405	25.444341176377282	22.910606066900336
28	23.621091208194844	25.06226611261943	25.75054505271896	25.56609762646677
29	23.010458440981164	24.200038596492554	28.309885271737055	24.479617690789222
30	23.262393080728984	24.88816647399892	27.790041123014426	24.059399322257676
31	23.820342768577017	24.735291129717826	27.801106457963627	23.643259643741533
32	25.14614361747326	25.55763812124964	27.467182595776517	21.82903566550059
33	26.28194548981857	25.79408884519823	26.267556777819784	21.65640888716342
34	28.012254197557397	24.031905930301985	25.13443626650312	22.821403605637506
35	28.093601403975743	24.698469622634313	25.055166170740762	22.152762802649185
36	24.38063392661908	25.51873950351013	27.74449575117574	22.356130818695053
37	24.092935217939893	26.499097967490577	27.573908317846268	21.834058496723262
38	25.751526959574516	27.653669367036404	24.3781036281836	22.21670004520548
39	26.07600940968893	27.22212130401762	25.015738833925184	21.686130452368264
40	26.16728898160775	25.25777887382081	26.600460967503036	21.9744711770684
41	23.388114923889	26.407893926868333	24.238635089045736	25.96535606019693
42	26.130958427952017	23.49559595892457	27.32163378726384	23.051811825859573
43	23.38127934154837	24.59623801271116	26.84121697535678	25.18126567038369
44	22.522941687195445	25.46322400052268	26.92732265345977	25.086511658822115
45	22.443822654026253	23.40012440004547	28.206180801530568	25.949872144397713
46	25.00365382647213	23.930693992882688	26.695781463788975	24.369870716856205
47	22.258317789621923	24.38297539681311	26.891936241011305	26.466770572553667
48	22.089580873058704	24.946589931904757	26.58984882033333	26.37398037470321
49	22.386607696865717	25.46873778517313	28.683123173794872	23.461531344166282
50	22.674042046006868	25.710362402937566	28.274687687530047	23.34090786352552
51	23.451259087831193	24.321190796209386	27.14810063336769	25.079449482591738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	745.0
1	1772.5
2	2800.0
3	6404.0
4	10008.0
5	6910.0
6	3812.0
7	3989.5
8	4167.0
9	4438.5
10	4710.0
11	4848.0
12	4986.0
13	5057.0
14	5128.0
15	5221.5
16	5315.0
17	5387.0
18	5459.0
19	7097.5
20	8736.0
21	9466.5
22	10197.0
23	13237.5
24	16278.0
25	17257.5
26	23327.0
27	28417.0
28	31327.5
29	34238.0
30	40830.0
31	47422.0
32	51396.0
33	55370.0
34	65743.0
35	76116.0
36	83073.5
37	90031.0
38	101867.0
39	113703.0
40	127960.5
41	142218.0
42	161858.5
43	181499.0
44	202018.5
45	222538.0
46	221742.5
47	220947.0
48	200969.0
49	180991.0
50	174024.5
51	167058.0
52	167739.0
53	168420.0
54	172717.0
55	177014.0
56	169020.5
57	161027.0
58	149904.5
59	138782.0
60	123684.0
61	108586.0
62	93944.5
63	79303.0
64	66571.0
65	53839.0
66	45974.0
67	38109.0
68	32648.5
69	27188.0
70	20694.5
71	14201.0
72	11783.0
73	9365.0
74	7604.0
75	4223.0
76	2603.0
77	2121.5
78	1640.0
79	1082.0
80	524.0
81	371.5
82	219.0
83	146.5
84	74.0
85	49.0
86	24.0
87	16.5
88	9.0
89	7.5
90	6.0
91	4.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2647909.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.803774646601426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92284202258631	12.25379003937257
2	10.189602663474032	3.4244757379085464
3	3.981361096885752	2.0070568397644215
4	2.142971893928583	1.4404006711830626
5	1.3403965399441582	1.1261860697152963
6	0.8741271840439286	0.8813181727885481
7	0.6601727035750692	0.7765375337099147
8	0.49048393427588877	0.65935851994804
9	0.3836682174102426	0.5802366837982498
>10	3.737934407195746	14.57787301426485
>50	1.7467564416988162	21.533732575147553
>100	1.5192645647019234	37.85814991285071
>500	0.005435650580610245	0.6398328291367705
>1k	0.004529708817175204	1.7563814575697099
>5k	4.5297088171752036E-4	0.48466994284182563
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	6847	0.25858139384699397	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5888	0.22236413713613268	No Hit
ATGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA	4412	0.1666220402589364	No Hit
ATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	3606	0.13618292773656498	No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA	3371	0.12730800038823084	No Hit
ATGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	3069	0.11590277460441427	No Hit
ATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	2960	0.11178631894071889	No Hit
ATGTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	2816	0.10634806558684608	No Hit
ATGGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTG	2787	0.10525286178641335	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.776564829078341E-5	0.0	0.0	0.10132523436417189	0.0
2	3.776564829078341E-5	0.0	0.0	0.45703987561506076	0.0
3	3.776564829078341E-5	0.0	0.0	0.6174683495543087	0.0
4	3.776564829078341E-5	0.0	0.0	0.8661929091974082	0.0
5	3.776564829078341E-5	0.0	0.0	1.8310674573786334	0.0
6	7.553129658156682E-5	0.0	0.0	2.063212897422079	0.0
7	7.553129658156682E-5	0.0	0.0	2.454540545011177	0.0
8	7.553129658156682E-5	0.0	0.0	3.6518248927738832	0.0
9	7.553129658156682E-5	0.0	0.0	3.823054342124295	0.0
10	7.553129658156682E-5	0.0	0.0	4.104030765407724	0.0
11	7.553129658156682E-5	0.0	0.0	4.332475171918673	0.0
12	7.553129658156682E-5	0.0	0.0	5.00583668094334	0.0
13	1.1329694487235022E-4	0.0	0.0	5.581649520432915	0.0
14	1.1329694487235022E-4	0.0	0.0	5.809036488791722	0.0
15	1.1329694487235022E-4	0.0	0.0	5.941782742533825	0.0
16	1.1329694487235022E-4	0.0	0.0	6.067693413935298	0.0
17	1.5106259316313364E-4	0.0	0.0	6.1933019601504435	0.0
18	1.8882824145391704E-4	0.0	0.0	6.445425428139713	0.0
19	1.8882824145391704E-4	0.0	0.0	6.766584501204536	0.0
20	2.2659388974470045E-4	0.0	0.0	7.115765685301119	0.0
21	3.021251863262673E-4	0.0	0.0	7.361997712157026	0.0
22	3.021251863262673E-4	0.0	0.0	7.652151187975115	0.0
23	3.398908346170507E-4	0.0	0.0	8.07799663810199	0.0
24	3.776564829078341E-4	0.0	0.0	8.455577589713242	0.0
25	4.154221311986175E-4	0.0	0.0	8.827040506301387	0.0
26	4.531877794894009E-4	0.0	0.0	9.140948574894379	0.0
27	4.531877794894009E-4	0.0	0.0	9.419998950114978	0.0
28	4.531877794894009E-4	0.0	0.0	9.68182818971498	0.0
29	4.531877794894009E-4	0.0	0.0	9.958839219927874	0.0
30	4.909534277801842E-4	0.0	0.0	10.225993415936877	0.0
31	5.287190760709676E-4	0.0	0.0	10.563014061283829	0.0
32	5.287190760709676E-4	0.0	0.0	10.98398774278119	0.0
33	5.664847243617512E-4	0.0	0.0	11.320706262941815	0.0
34	6.042503726525346E-4	0.0	0.0	11.670680525652505	0.0
35	6.042503726525346E-4	0.0	0.0	12.005095341267392	0.0
36	6.797816692341014E-4	0.0	0.0	12.383469371492751	0.0
37	7.175473175248848E-4	0.0	0.0	12.793302186744333	0.0
38	7.175473175248848E-4	0.0	0.0	13.128736674863072	0.0
39	7.175473175248848E-4	0.0	0.0	13.47908859405667	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	35	1.2127384E-7	45.0	3
CGTCGGT	20	7.0349546E-4	45.0	36
CCGCGTA	25	3.89219E-5	45.0	36
CGTCCTA	20	7.0349546E-4	45.0	43
ATCGCTA	130	0.0	43.269234	1
ATTACGC	140	0.0	41.785713	1
CGCGTAA	95	0.0	40.263157	33
CGTAATC	40	3.4605E-7	39.375	45
TCGTTGC	180	0.0	38.75	2
CGAATCC	35	6.2513755E-6	38.571426	39
CGTTTTT	3180	0.0	38.490562	1
TTCGCGA	65	9.094947E-12	38.076927	2
ATCTCGT	210	0.0	37.500004	1
CGGTAAG	60	1.5643309E-10	37.499996	3
ATGTTTT	5145	0.0	37.478134	1
ATTAGTT	1655	0.0	36.570995	1
AACGTCC	25	0.0021076042	36.0	13
ACGATTG	50	4.883077E-8	36.0	3
TTACGCG	50	4.883077E-8	36.0	3
TAGGCGT	25	0.0021076042	36.0	36
>>END_MODULE