FastQCFastQC Report
Thu 26 May 2016
SRR1545681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545681_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2134575
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC53090.24871461532155115No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT52190.24449831933757304No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTT50970.238782895892625Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC46860.21952847756579177No Hit
GGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC39390.18453322089877375No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC38460.18017638171532976No Hit
ATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC37550.17591323799819636No Hit
TTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC34610.16214000445053464No Hit
TGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC31590.14799198903763044No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG29940.14026211306700395No Hit
AGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC28340.13276647576215406No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA206.3126767E-446.045
AACATCG253.418147E-546.024
CATACCG206.3126767E-446.01
ACGTACG206.3126767E-446.038
CAATACG253.418147E-546.013
TCGGTAA206.3126767E-446.016
TTACGCG800.040.251
TCAAGCG1550.040.06451417
TTTACGG3550.039.5211262
CTTAACG355.375279E-639.428571
GTAACGT355.375279E-639.4285728
TACGGGT1650.039.0303044
AACGGGC1650.039.0303044
TAGGGCG10600.038.8396225
CAAACGT301.0018537E-438.33333242
CGCCTAC301.0018537E-438.33333217
CGTAGCG301.0018537E-438.3333321
CGGCTAT601.2369128E-1038.33333240
CCAACCG301.0018537E-438.33333210
GTTTACG1150.038.01
Produced by FastQC (version 0.11.5)