##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545681_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2134575
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53179204291252	33.0	31.0	34.0	31.0	34.0
2	32.94793577175784	34.0	31.0	34.0	31.0	34.0
3	33.036001545975196	34.0	33.0	34.0	31.0	34.0
4	36.423425740486984	37.0	37.0	37.0	35.0	37.0
5	36.388092243186584	37.0	37.0	37.0	35.0	37.0
6	36.38716840588876	37.0	37.0	37.0	35.0	37.0
7	36.45386318119532	37.0	37.0	37.0	35.0	37.0
8	36.49697574458616	37.0	37.0	37.0	35.0	37.0
9	38.315419228652075	39.0	39.0	39.0	37.0	39.0
10	38.08679807455817	39.0	39.0	39.0	35.0	39.0
11	37.92126723118185	39.0	38.0	39.0	35.0	39.0
12	37.529723715493716	39.0	37.0	39.0	35.0	39.0
13	37.427395148917235	39.0	37.0	39.0	35.0	39.0
14	38.50746167269831	41.0	38.0	41.0	34.0	41.0
15	38.646115971563425	41.0	38.0	41.0	34.0	41.0
16	38.773548364428514	41.0	38.0	41.0	35.0	41.0
17	38.75182694447373	40.0	38.0	41.0	35.0	41.0
18	38.724634177763726	40.0	38.0	41.0	35.0	41.0
19	38.70363140203553	40.0	38.0	41.0	35.0	41.0
20	38.57182108850708	40.0	38.0	41.0	34.0	41.0
21	38.56027218532963	40.0	38.0	41.0	34.0	41.0
22	38.728902006254174	40.0	38.0	41.0	35.0	41.0
23	38.75218767201902	40.0	38.0	41.0	35.0	41.0
24	38.70417389878547	40.0	38.0	41.0	35.0	41.0
25	38.63132520525163	40.0	37.0	41.0	35.0	41.0
26	38.50885492428235	40.0	37.0	41.0	35.0	41.0
27	38.43697035709685	40.0	37.0	41.0	35.0	41.0
28	38.37778152559643	40.0	37.0	41.0	35.0	41.0
29	38.304878020214794	40.0	36.0	41.0	34.0	41.0
30	38.226215991473715	40.0	36.0	41.0	34.0	41.0
31	38.152434091095415	40.0	36.0	41.0	34.0	41.0
32	38.11456425752199	40.0	36.0	41.0	34.0	41.0
33	38.03320286239649	40.0	36.0	41.0	34.0	41.0
34	37.89243432533408	40.0	35.0	41.0	34.0	41.0
35	37.794806460302404	40.0	35.0	41.0	33.0	41.0
36	37.73051450522938	40.0	35.0	41.0	33.0	41.0
37	37.64221214995959	40.0	35.0	41.0	33.0	41.0
38	37.53871848025954	40.0	35.0	41.0	33.0	41.0
39	37.440616516168326	40.0	35.0	41.0	33.0	41.0
40	37.31334902732394	39.0	35.0	41.0	33.0	41.0
41	37.16578241570336	39.0	35.0	41.0	33.0	41.0
42	37.0883159411124	39.0	35.0	41.0	33.0	41.0
43	37.01563636789525	39.0	35.0	41.0	33.0	41.0
44	36.91777192181113	39.0	35.0	41.0	32.0	41.0
45	36.86950845015987	39.0	35.0	41.0	32.0	41.0
46	36.85326821498425	39.0	35.0	41.0	33.0	41.0
47	36.803270908728905	38.0	35.0	41.0	33.0	41.0
48	36.69462351990443	38.0	35.0	41.0	32.0	41.0
49	36.60408699624047	38.0	35.0	40.0	32.0	41.0
50	36.507468231380955	38.0	35.0	40.0	32.0	41.0
51	36.401811133363786	38.0	35.0	40.0	32.0	41.0
52	36.07448040007964	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	5.0
11	5.0
12	14.0
13	22.0
14	39.0
15	76.0
16	124.0
17	191.0
18	409.0
19	690.0
20	1263.0
21	1878.0
22	2658.0
23	3692.0
24	4752.0
25	6532.0
26	8600.0
27	10850.0
28	13330.0
29	17224.0
30	22031.0
31	29007.0
32	38033.0
33	56088.0
34	138305.0
35	197078.0
36	127569.0
37	197674.0
38	334307.0
39	920967.0
40	1154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.60486279470152	13.453076139278311	45.07248515512456	11.869575910895612
2	25.99435484815478	12.876567935069042	48.651558272724074	12.477518944052095
3	26.719932539264256	13.991684527364932	44.46243397397608	14.825948959394728
4	24.646779803942238	14.171158193082933	46.04790180715131	15.134160195823524
5	24.348406591476053	16.209315671737933	44.15848588126442	15.283791855521592
6	26.226110584074114	19.07283651312322	42.928592342738014	11.77246056006465
7	86.43940831313024	2.8902708970169706	6.742653689844583	3.927667100008198
8	87.37748732183222	2.799479990161976	6.809411709590902	3.013620978414907
9	80.58358221191571	4.665565744937517	8.521368422285466	6.229483620861295
10	45.91237131513299	20.28708290877575	15.357389644308586	18.443156131782672
11	35.51311150931684	22.650738437393862	23.344600213157186	18.49154984013211
12	30.600939297049763	21.79023927479709	26.933792441118253	20.675028987034892
13	26.634388578522657	21.961139805347667	32.51335745991591	18.891114156213767
14	20.254992211564364	27.054097419861094	33.31220500568029	19.37870536289425
15	20.215686963447055	21.566541348980476	37.23509363690665	20.98267805066582
16	26.534415515969222	21.08311959055081	31.20269842942975	21.17976646405022
17	26.603703313305928	21.363315882552733	27.253621915369568	24.779358888771768
18	26.656922338170364	21.19264959066793	29.472611644004076	22.677816427157634
19	27.820666877481464	24.28361617652226	26.595692350936368	21.30002459505991
20	26.214586041717908	25.09895412435731	25.46118079711418	23.225279036810605
21	26.902966632702057	23.91305060726374	28.86808849536793	20.31589426466627
22	28.448754435894735	20.59871402972489	27.580853331459426	23.371678202920958
23	27.79794572690114	22.629094784676106	25.674572221636627	23.898387266786127
24	27.222327629621823	21.71101975803146	26.83105536230866	24.23559725003806
25	31.053019921998526	22.010236229694435	23.295831722942506	23.640912125364533
26	23.604089807104458	24.969654380848645	24.317627630793016	27.10862818125388
27	22.934588852581896	23.408032043849477	27.402597706803462	26.254781396765164
28	23.56478455898715	24.364943841279878	26.708735931040138	25.36153566869283
29	24.376140449504	24.562547579728985	26.839019476945058	24.222292493821957
30	26.36337444221918	25.70239977513088	25.778293102842486	22.155932679807457
31	25.697902392747974	27.022568895447574	24.852347657027746	22.427181054776714
32	29.033273602473557	23.49544991391729	23.816263190564865	23.655013293044284
33	29.50268788868979	24.645702306079663	23.571343241628895	22.280266563601653
34	24.4295000175679	25.813522598175282	26.906386517222398	22.85059086703442
35	23.78286075682513	26.784582411018587	26.708970169705914	22.72358666245037
36	26.291931649157323	27.490109272337587	23.673002822575924	22.544956255929165
37	27.751425927877914	26.58430835177963	23.83242565850345	21.83184006183901
38	26.43280278275535	24.457280723329	26.82299755220594	22.286918941709708
39	23.910192895541265	25.778199407376174	23.317897005258658	26.993710691823896
40	27.127273579049692	22.760877458042	27.010341637094037	23.10150732581427
41	24.275979996017945	23.711839593361674	25.903657636766102	26.108522773854283
42	22.889146551421245	24.847990817844302	26.589321059227245	25.673541571507208
43	22.52654509679913	22.26691495965239	28.259114812082032	26.947425131466453
44	26.98598081585327	22.5725964184908	25.322933136572857	25.118489629083072
45	22.347165126547438	23.237412599697834	26.341824484967734	28.073597788786998
46	22.206762470280967	24.300762446857103	25.787381563074618	27.70509351978731
47	22.86127215019383	24.30582200203788	28.98450511225888	23.84840073550941
48	22.671913612780063	24.797301570570255	29.086914256936392	23.443870559713293
49	23.896934987058312	22.916037150252393	26.283780143588302	26.903247719100992
50	21.813709989107902	24.02356440977712	28.04577023529274	26.116955365822236
51	23.86760830610309	24.545963482192004	26.230514270990714	25.355913940714192
52	22.143143248656056	23.909724418209713	27.168124802361127	26.779007530773104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	123.0
2	87.0
3	89.5
4	92.0
5	110.0
6	128.0
7	240.0
8	352.0
9	438.5
10	525.0
11	709.5
12	894.0
13	906.5
14	1233.5
15	1548.0
16	1541.5
17	1535.0
18	1690.0
19	1845.0
20	2568.0
21	3291.0
22	4689.0
23	6087.0
24	7385.5
25	8684.0
26	10168.0
27	11652.0
28	14291.0
29	16930.0
30	19358.5
31	21787.0
32	25386.0
33	28985.0
34	35471.5
35	41958.0
36	46123.5
37	50289.0
38	56050.5
39	70684.0
40	79556.0
41	88164.5
42	96773.0
43	114601.5
44	132430.0
45	156097.5
46	179765.0
47	191460.5
48	203156.0
49	188506.5
50	173857.0
51	155443.5
52	137030.0
53	136360.5
54	135691.0
55	135311.5
56	134932.0
57	131121.0
58	127310.0
59	123449.5
60	119589.0
61	108279.5
62	96970.0
63	88700.0
64	68617.0
65	56804.0
66	49784.5
67	42765.0
68	34801.5
69	26838.0
70	23769.5
71	20701.0
72	16366.0
73	12031.0
74	9907.5
75	7784.0
76	6559.0
77	5334.0
78	3883.5
79	2433.0
80	1950.5
81	1468.0
82	1154.0
83	840.0
84	526.5
85	213.0
86	157.5
87	102.0
88	110.5
89	81.0
90	43.0
91	29.0
92	15.0
93	15.5
94	16.0
95	11.0
96	6.0
97	6.0
98	6.0
99	7.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2134575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.817204591829498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.83386770065617	12.442443082613448
2	11.682318143019465	4.162925049220389
3	4.574011416018127	2.4448829161367613
4	2.383454229221101	1.6986596654917456
5	1.4535664670942632	1.2949245566020642
6	0.9365900818889284	1.0012450264616026
7	0.7120577773394886	0.888081537004273
8	0.5252646942255876	0.7486998817505645
9	0.43168254680744694	0.6922238629671266
>10	4.254117873750764	17.811107505852945
>50	2.057533893458231	26.901914284771884
>100	1.1455168474929147	26.70311169379549
>500	0.005536444988889255	0.6739262294078611
>1k	0.0036909633259261695	1.801896574899669
>5k	7.90920712698465E-4	0.7339581330241743
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	5309	0.24871461532155115	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	5219	0.24449831933757304	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTT	5097	0.238782895892625	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	4686	0.21952847756579177	No Hit
GGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	3939	0.18453322089877375	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	3846	0.18017638171532976	No Hit
ATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	3755	0.17591323799819636	No Hit
TTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	3461	0.16214000445053464	No Hit
TGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	3159	0.14799198903763044	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	2994	0.14026211306700395	No Hit
AGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	2834	0.13276647576215406	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2516191747771805	0.0
2	0.0	0.0	0.0	0.6319290725320028	0.0
3	0.0	0.0	0.0	2.3629528126207795	0.0
4	0.0	0.0	0.0	2.7453708583675906	0.0
5	0.0	0.0	0.0	3.424194511788061	0.0
6	0.0	0.0	0.0	5.512713303585023	0.0
7	0.0	0.0	0.0	5.832777016502114	0.0
8	0.0	0.0	0.0	6.329878313013129	0.0
9	0.0	0.0	0.0	6.676457842896127	0.0
10	0.0	0.0	0.0	7.889767283885551	0.0
11	0.0	0.0	0.0	8.867947952168464	0.0
12	0.0	0.0	0.0	9.252708384573042	0.0
13	0.0	0.0	0.0	9.450780600353701	0.0
14	4.684773315531195E-5	0.0	0.0	9.59427520700842	0.0
15	4.684773315531195E-5	0.0	0.0	9.777918320977244	0.0
16	4.684773315531195E-5	0.0	0.0	10.175233945867443	0.0
17	4.684773315531195E-5	0.0	0.0	10.696977150018153	0.0
18	4.684773315531195E-5	0.0	0.0	11.241394657016034	0.0
19	4.684773315531195E-5	0.0	0.0	11.617909888385276	0.0
20	4.684773315531195E-5	0.0	0.0	12.080859187426068	0.0
21	4.684773315531195E-5	0.0	0.0	12.74426993663844	0.0
22	4.684773315531195E-5	0.0	0.0	13.325322370963775	0.0
23	4.684773315531195E-5	0.0	0.0	13.880233770188445	0.0
24	1.873909326212478E-4	0.0	0.0	14.347680451612147	0.0
25	1.873909326212478E-4	0.0	0.0	14.759190939648407	0.0
26	1.873909326212478E-4	0.0	0.0	15.128725858777509	0.0
27	1.873909326212478E-4	0.0	0.0	15.531241582047949	0.0
28	1.873909326212478E-4	0.0	0.0	15.89927737371608	0.0
29	1.873909326212478E-4	0.0	0.0	16.332712600869026	0.0
30	1.873909326212478E-4	0.0	0.0	16.86986870922783	0.0
31	1.873909326212478E-4	0.0	0.0	17.30100839745617	0.0
32	1.873909326212478E-4	0.0	0.0	17.78663199934413	0.0
33	1.873909326212478E-4	0.0	0.0	18.23196655071853	0.0
34	1.873909326212478E-4	0.0	0.0	18.742044669313564	0.0
35	1.873909326212478E-4	0.0	0.0	19.281168382464895	0.0
36	1.873909326212478E-4	0.0	0.0	19.700924071536488	0.0
37	1.873909326212478E-4	0.0	0.0	20.155440778609325	0.0
38	1.873909326212478E-4	0.0	0.0	20.71189815302812	0.0
39	1.873909326212478E-4	0.0	0.0	21.792675356921166	0.0
40	1.873909326212478E-4	0.0	0.0	22.274832226555638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	20	6.3126767E-4	46.0	45
AACATCG	25	3.418147E-5	46.0	24
CATACCG	20	6.3126767E-4	46.0	1
ACGTACG	20	6.3126767E-4	46.0	38
CAATACG	25	3.418147E-5	46.0	13
TCGGTAA	20	6.3126767E-4	46.0	16
TTACGCG	80	0.0	40.25	1
TCAAGCG	155	0.0	40.064514	17
TTTACGG	355	0.0	39.521126	2
CTTAACG	35	5.375279E-6	39.42857	1
GTAACGT	35	5.375279E-6	39.42857	28
TACGGGT	165	0.0	39.030304	4
AACGGGC	165	0.0	39.030304	4
TAGGGCG	1060	0.0	38.839622	5
CAAACGT	30	1.0018537E-4	38.333332	42
CGCCTAC	30	1.0018537E-4	38.333332	17
CGTAGCG	30	1.0018537E-4	38.333332	1
CGGCTAT	60	1.2369128E-10	38.333332	40
CCAACCG	30	1.0018537E-4	38.333332	10
GTTTACG	115	0.0	38.0	1
>>END_MODULE