SRR1545680_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545680_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2157250
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	7046	0.3266195387646309	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5331	0.2471201761501912	No Hit
ATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	3549	0.16451500753273846	No Hit
ATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	3168	0.14685363309769384	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	2954	0.13693359601344304	Illumina Paired End PCR Primer 2 (95% over 21bp)
ATTGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	2654	0.12302700197010083	No Hit
ATGCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	2594	0.12024568316143239	No Hit
ATGTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	2488	0.1153320199327848	No Hit
ATATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	2434	0.1128288330049832	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	20	7.0346624E-4	45.000004	19
CATATCG	45	3.8562575E-10	45.000004	28
CGTATGT	20	7.0346624E-4	45.000004	14
CTATACG	20	7.0346624E-4	45.000004	3
CGCGATC	35	1.2125929E-7	45.000004	18
ACAATCG	40	6.8193913E-9	45.000004	26
CATGTAC	20	7.0346624E-4	45.000004	20
CGTCGAA	65	0.0	45.000004	33
CGCAACA	20	7.0346624E-4	45.000004	38
CGATAAG	35	1.2125929E-7	45.000004	3
GTACGAG	45	3.8562575E-10	45.000004	3
TCACGTA	40	6.8193913E-9	45.000004	2
TCGCAAT	50	2.1827873E-11	45.0	2
ACGTGTA	25	3.8919476E-5	45.0	36
CGTACGA	25	3.8919476E-5	45.0	4
CAGTCGA	25	3.8919476E-5	45.0	45
TCGACTA	50	2.1827873E-11	45.0	2
AATACGC	25	3.8919476E-5	45.0	38
ACGCCTA	30	2.166391E-6	44.999996	15
CGATTAC	30	2.166391E-6	44.999996	45