##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545680_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2157250
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.859262950515703	31.0	31.0	34.0	31.0	34.0
2	32.527701935334335	34.0	31.0	34.0	31.0	34.0
3	32.495865106037776	34.0	31.0	34.0	31.0	34.0
4	35.989348939622204	37.0	35.0	37.0	35.0	37.0
5	35.757872754664504	37.0	35.0	37.0	35.0	37.0
6	35.77137466682118	37.0	35.0	37.0	35.0	37.0
7	35.757959902653845	37.0	35.0	37.0	33.0	37.0
8	35.71856993857921	37.0	35.0	37.0	33.0	37.0
9	37.89292108007881	39.0	38.0	39.0	35.0	39.0
10	37.821109746204655	39.0	38.0	39.0	35.0	39.0
11	37.61460192374551	39.0	37.0	39.0	35.0	39.0
12	37.15503720013906	39.0	37.0	39.0	33.0	39.0
13	36.89194900915518	39.0	37.0	39.0	33.0	39.0
14	37.60869069417082	40.0	35.0	41.0	33.0	41.0
15	37.50331394136053	40.0	35.0	41.0	32.0	41.0
16	37.515079847027465	40.0	35.0	41.0	32.0	41.0
17	37.58359949009155	40.0	35.0	41.0	33.0	41.0
18	37.62268536330977	40.0	35.0	41.0	33.0	41.0
19	37.55783613396686	40.0	35.0	41.0	32.0	41.0
20	37.51023247189709	39.0	35.0	41.0	32.0	41.0
21	37.49819492409318	39.0	35.0	41.0	32.0	41.0
22	37.433549658129564	39.0	35.0	41.0	32.0	41.0
23	37.369462510140224	39.0	35.0	41.0	32.0	41.0
24	37.333036504809364	39.0	35.0	41.0	32.0	41.0
25	37.27838776219724	39.0	35.0	41.0	32.0	41.0
26	37.18231776567389	39.0	35.0	41.0	32.0	41.0
27	37.08601506547688	39.0	35.0	41.0	32.0	41.0
28	36.99084250782246	39.0	35.0	41.0	31.0	41.0
29	36.90809085641442	39.0	35.0	41.0	31.0	41.0
30	36.82247583729285	39.0	35.0	41.0	31.0	41.0
31	36.722605632170584	39.0	35.0	41.0	31.0	41.0
32	36.6027057596477	39.0	35.0	41.0	31.0	41.0
33	36.521042067446984	39.0	35.0	41.0	30.0	41.0
34	36.418646888399586	38.0	35.0	41.0	30.0	41.0
35	36.31438915285665	38.0	35.0	41.0	30.0	41.0
36	36.22058314984355	38.0	35.0	41.0	30.0	41.0
37	36.07024406072546	38.0	35.0	41.0	30.0	41.0
38	36.03853424498783	38.0	35.0	40.0	30.0	41.0
39	35.966324255417774	38.0	35.0	40.0	30.0	41.0
40	35.897829644222966	38.0	35.0	40.0	30.0	41.0
41	35.794658477227955	38.0	35.0	40.0	29.0	41.0
42	35.66220558581527	38.0	34.0	40.0	29.0	41.0
43	35.57552717580253	37.0	34.0	40.0	29.0	41.0
44	35.5266204658709	37.0	34.0	40.0	29.0	41.0
45	35.483334801251594	37.0	34.0	40.0	28.0	41.0
46	35.39399281492641	37.0	34.0	40.0	28.0	41.0
47	35.29291551744119	37.0	34.0	40.0	28.0	41.0
48	35.21580159925831	36.0	34.0	40.0	28.0	41.0
49	35.16183149843551	36.0	34.0	40.0	28.0	41.0
50	35.03050550469347	36.0	34.0	40.0	28.0	41.0
51	34.85029690578283	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	9.0
11	24.0
12	29.0
13	32.0
14	35.0
15	64.0
16	142.0
17	279.0
18	636.0
19	1187.0
20	2062.0
21	3506.0
22	5294.0
23	7863.0
24	11283.0
25	15719.0
26	21267.0
27	26227.0
28	31203.0
29	37327.0
30	44638.0
31	55185.0
32	69048.0
33	88700.0
34	145240.0
35	257611.0
36	166057.0
37	225461.0
38	334794.0
39	606059.0
40	261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.322725692432495	90.5205238150423	3.713338741453239	3.4434117510719666
2	3.6724997102792907	2.76004171978213	90.59242090624637	2.9750376636922007
3	27.736748174759533	16.188805191795108	42.767643991192486	13.306802642252869
4	27.15930003476649	15.2672615598563	43.70119364932205	13.872244756055164
5	24.435786302004868	14.349101865801368	46.54305249739251	14.672059334801252
6	23.11632865917256	14.662695561478733	46.74511530884228	15.475860470506431
7	23.368872406999653	17.556379650017384	43.94251941128752	15.132228531695446
8	26.72281840305945	19.862695561478734	42.08464480241047	11.329841233051338
9	82.63381620118207	4.073612237802758	8.829852821879708	4.4627187391354735
10	83.21005910302468	4.458732182176382	8.563541545949704	3.767667168849229
11	76.09224707382084	7.177007764515007	10.116861745277554	6.613883416386604
12	45.010174991308375	21.347966160621162	15.944049136632287	17.697809711438172
13	35.23036273032796	23.57422644570634	21.970610731255068	19.224800092710627
14	29.749634951906362	22.811217985861628	26.653841696604474	20.785305365627536
15	23.75705180206281	24.374319156333293	31.12601691968942	20.742612121914476
16	19.08077413373508	30.210082280681423	31.08814462857805	19.620998957005447
17	19.68691621277089	22.865870900451966	37.44623942519411	20.000973461583033
18	24.229227025147758	20.102955151234212	35.024962336307794	20.642855487310232
19	23.773647004287866	20.497531579557307	28.856785259010316	26.872036157144514
20	24.428693939042763	21.86765558002086	30.8578514312203	22.845799049716074
21	27.827233746668213	23.903766369220072	26.901564491829877	21.36743539228184
22	28.421184378259355	21.99717232587785	26.239610615366786	23.342032680496004
23	27.371190172673543	24.011542473055975	27.684505736470044	20.93276161780044
24	28.5742032680496	21.503627303279636	25.22368756518716	24.6984818634836
25	27.41379070575965	23.456530304786185	24.66950979255997	24.460169196894192
26	28.113755939274537	20.386603314404912	28.10939853980762	23.39024220651292
27	29.145393440723144	21.062834627419168	25.53408274423456	24.257689187623132
28	24.131927222157838	24.996731950399813	25.230965349403174	25.640375478039168
29	23.680055626376173	24.585792096419052	26.32110325646077	25.413049020744005
30	24.35688955846564	24.975315795573067	27.566855950863367	23.100938695097923
31	25.119712597056438	23.60171514659868	27.319364932205353	23.959207324139527
32	24.936655464132578	24.756982269092596	26.875559160968827	23.430803105806003
33	28.797125970564373	24.349843550817013	24.32550701124116	22.527523467377446
34	29.922308494611194	22.769683624985515	25.80322169428671	21.504786186116583
35	26.913199675512807	24.821647931394136	24.89136632286476	23.3737860702283
36	25.17343840537722	24.896372696720363	26.375014486035465	23.55517441186696
37	24.62153204311044	24.07273148684668	28.983659751999074	22.322076718043807
38	26.65861629389269	26.85854676092247	24.30214393324835	22.18069301193649
39	26.294031753389735	29.056993857920965	24.079592073241397	20.56938231544791
40	26.044686522192606	26.185050411403406	25.55392281840306	22.216340248000925
41	24.707243017730907	25.977193185768922	24.926781782361804	24.38878201413837
42	25.97668327732066	23.04716653146367	27.19721868119133	23.778931510024336
43	22.016178004403756	24.58393788387994	27.51391818287171	25.885965928844595
44	22.71058060030131	25.282419747363544	25.334337698458686	26.672661953876464
45	23.31166995016804	22.703905435160504	26.63173021207556	27.352694402595894
46	24.97907057596477	23.186927801599257	26.654907868814465	25.17909375362151
47	22.782709468072778	24.233677135241628	26.520384749101865	26.46322864758373
48	22.835183682929657	25.185351720941014	25.38634836018079	26.593116235948543
49	23.74991308378723	24.49665082860123	28.53466218565303	23.21877390195851
50	23.90358094796616	22.892015297253447	29.942009502839262	23.262394251941128
51	23.2169196894194	23.25479198053077	27.83766369220072	25.690624637849112
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	625.0
1	1724.5
2	2824.0
3	6261.0
4	9698.0
5	6696.0
6	3694.0
7	3724.5
8	3755.0
9	3940.0
10	4125.0
11	4475.0
12	4825.0
13	5020.5
14	5216.0
15	5086.0
16	4956.0
17	5404.0
18	5852.0
19	5979.0
20	6106.0
21	6351.0
22	6596.0
23	7392.0
24	8188.0
25	11000.5
26	17213.5
27	20614.0
28	21197.0
29	21780.0
30	23863.5
31	25947.0
32	31631.0
33	37315.0
34	41959.5
35	46604.0
36	52951.5
37	59299.0
38	70084.5
39	80870.0
40	98458.5
41	116047.0
42	126658.0
43	137269.0
44	150163.0
45	163057.0
46	181056.0
47	199055.0
48	194457.5
49	189860.0
50	171092.5
51	152325.0
52	145965.5
53	139606.0
54	139852.5
55	140099.0
56	138569.0
57	137039.0
58	131669.5
59	126300.0
60	109178.0
61	92056.0
62	78987.0
63	65918.0
64	55410.0
65	44902.0
66	36932.5
67	28963.0
68	24038.5
69	19114.0
70	16513.5
71	13913.0
72	11361.0
73	8809.0
74	6898.5
75	3836.0
76	2684.0
77	1929.0
78	1174.0
79	1072.5
80	971.0
81	636.5
82	302.0
83	170.5
84	39.0
85	28.5
86	18.0
87	16.5
88	15.0
89	10.0
90	5.0
91	5.5
92	6.0
93	6.0
94	6.0
95	7.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2157250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.335786044333844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63721355679996	11.865859795204697
2	10.620331350252608	3.469829213153155
3	4.431711434653177	2.171864694201663
4	2.42048415808101	1.5816204532044367
5	1.4823439224712096	1.2107626580804136
6	0.968506811094638	0.9492792029113236
7	0.6856165905918946	0.7840060152648374
8	0.48455935742795253	0.6332526390978337
9	0.35955724297706715	0.5286285172767534
>10	2.8561278403073374	10.574675821473447
>50	1.2789275080575824	15.529401568959274
>100	1.758694148324854	47.26431827596034
>500	0.009669405083346998	0.9978257617528683
>1k	0.005687885343145294	1.8636642346920715
>5k	5.687885343145293E-4	0.5750111487668894
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	7046	0.3266195387646309	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5331	0.2471201761501912	No Hit
ATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	3549	0.16451500753273846	No Hit
ATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	3168	0.14685363309769384	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	2954	0.13693359601344304	Illumina Paired End PCR Primer 2 (95% over 21bp)
ATTGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	2654	0.12302700197010083	No Hit
ATGCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	2594	0.12024568316143239	No Hit
ATGTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	2488	0.1153320199327848	No Hit
ATATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	2434	0.1128288330049832	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1485687796963727	0.0
2	0.0	0.0	0.0	0.6197705411982849	0.0
3	0.0	0.0	0.0	0.8546529145903349	0.0
4	0.0	0.0	0.0	1.159809943214741	0.0
5	0.0	0.0	0.0	2.2981573762892573	0.0
6	0.0	0.0	0.0	2.565488469115772	0.0
7	0.0	0.0	0.0	3.0258430872638775	0.0
8	0.0	0.0	0.0	4.359531811333874	0.0
9	0.0	0.0	0.0	4.529284969289605	0.0
10	0.0	0.0	0.0	4.848070460076486	0.0
11	4.635531347780739E-5	0.0	0.0	5.101819446054004	0.0
12	1.3906594043342217E-4	0.0	0.0	5.837663692200718	0.0
13	1.8542125391122957E-4	0.0	0.0	6.4544674933364234	0.0
14	1.8542125391122957E-4	0.0	0.0	6.704971607370495	0.0
15	1.8542125391122957E-4	0.0	0.0	6.851871595781667	0.0
16	1.8542125391122957E-4	0.0	0.0	6.967759879476185	0.0
17	1.8542125391122957E-4	0.0	0.0	7.112342102213466	0.0
18	1.8542125391122957E-4	0.0	0.0	7.419446054003941	0.0
19	1.8542125391122957E-4	0.0	0.0	7.7734615830339555	0.0
20	2.3177656738903696E-4	0.0	0.0	8.181527407579093	0.0
21	2.3177656738903696E-4	0.0	0.0	8.460586394715495	0.0
22	2.7813188086684435E-4	0.0	0.0	8.7847491018658	0.0
23	3.7084250782245913E-4	0.0	0.0	9.232031521613164	0.0
24	4.171978213002665E-4	0.0	0.0	9.607509560783404	0.0
25	5.099084482558813E-4	0.0	0.0	10.004357399466913	0.0
26	5.562637617336887E-4	0.0	0.0	10.34312203036273	0.0
27	5.562637617336887E-4	0.0	0.0	10.631961988642948	0.0
28	5.562637617336887E-4	0.0	0.0	10.903974968130722	0.0
29	6.026190752114961E-4	0.0	0.0	11.156472360644338	0.0
30	6.026190752114961E-4	0.0	0.0	11.421068489975664	0.0
31	6.026190752114961E-4	0.0	0.0	11.702259821532044	0.0
32	6.489743886893035E-4	0.0	0.0	12.005516282303859	0.0
33	6.489743886893035E-4	0.0	0.0	12.309329006837409	0.0
34	6.489743886893035E-4	0.0	0.0	12.603824313361919	0.0
35	6.489743886893035E-4	0.0	0.0	12.905411982848534	0.0
36	6.489743886893035E-4	0.0	0.0	13.203059450689535	0.0
37	6.489743886893035E-4	0.0	0.0	13.543261096303164	0.0
38	6.489743886893035E-4	0.0	0.0	13.862927338046124	0.0
39	6.489743886893035E-4	0.0	0.0	14.182083671340827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	20	7.0346624E-4	45.000004	19
CATATCG	45	3.8562575E-10	45.000004	28
CGTATGT	20	7.0346624E-4	45.000004	14
CTATACG	20	7.0346624E-4	45.000004	3
CGCGATC	35	1.2125929E-7	45.000004	18
ACAATCG	40	6.8193913E-9	45.000004	26
CATGTAC	20	7.0346624E-4	45.000004	20
CGTCGAA	65	0.0	45.000004	33
CGCAACA	20	7.0346624E-4	45.000004	38
CGATAAG	35	1.2125929E-7	45.000004	3
GTACGAG	45	3.8562575E-10	45.000004	3
TCACGTA	40	6.8193913E-9	45.000004	2
TCGCAAT	50	2.1827873E-11	45.0	2
ACGTGTA	25	3.8919476E-5	45.0	36
CGTACGA	25	3.8919476E-5	45.0	4
CAGTCGA	25	3.8919476E-5	45.0	45
TCGACTA	50	2.1827873E-11	45.0	2
AATACGC	25	3.8919476E-5	45.0	38
ACGCCTA	30	2.166391E-6	44.999996	15
CGATTAC	30	2.166391E-6	44.999996	45
>>END_MODULE