FastQCFastQC Report
Thu 26 May 2016
SRR1545679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545679_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1796601
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT64040.356450875848338Illumina Paired End PCR Primer 2 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC53880.29989964382742745TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT51760.28809958360259175TruSeq Adapter, Index 22 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC49480.27540895279474964No Hit
GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC42010.23383043870063525TruSeq Adapter, Index 22 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC38690.2153510991032511No Hit
ATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC37030.206111429304559No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC34910.19431136907972332TruSeq Adapter, Index 22 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC26890.1496715186065242No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC20470.11393737396338975No Hit
TCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC19390.1079260225280961No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT19300.10742507657515497TruSeq Adapter, Index 22 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC351.0199983E-746.00000446
GACGTAT351.0199983E-746.00000435
GCTATAA351.0199983E-746.00000440
CTCGATT351.0199983E-746.00000442
AACGTAG206.3124084E-446.037
ATAATCG206.3124084E-446.016
TAGCGTA253.4179295E-546.024
CGCGATA206.3124084E-446.01
TAACGCG253.4179295E-546.034
ACCTATC206.3124084E-446.039
ACGATAG253.4179295E-546.01
CGGATTA253.4179295E-546.022
TAACACG405.6152203E-946.025
ACTACGT253.4179295E-546.038
GTCGTAC301.8619921E-646.033
CGAATGT301.8619921E-646.018
TCGTATT253.4179295E-546.014
CTATTCG206.3124084E-446.046
CGTCGAT253.4179295E-546.030
ACCGTTC253.4179295E-546.015
Produced by FastQC (version 0.11.5)