##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545679_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1796601
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52990619508728	33.0	31.0	34.0	31.0	34.0
2	32.94465771754552	34.0	31.0	34.0	31.0	34.0
3	33.01932259861817	34.0	33.0	34.0	31.0	34.0
4	36.41383479136436	37.0	37.0	37.0	35.0	37.0
5	36.39584526558763	37.0	37.0	37.0	35.0	37.0
6	36.393298790326845	37.0	37.0	37.0	35.0	37.0
7	36.4780632984174	37.0	37.0	37.0	35.0	37.0
8	36.50408076139332	37.0	37.0	37.0	35.0	37.0
9	38.29370071596309	39.0	39.0	39.0	37.0	39.0
10	38.05091725987017	39.0	39.0	39.0	35.0	39.0
11	37.87667601209172	39.0	38.0	39.0	35.0	39.0
12	37.504650726566446	39.0	37.0	39.0	35.0	39.0
13	37.42432960907848	39.0	37.0	39.0	35.0	39.0
14	38.719306067401725	41.0	38.0	41.0	35.0	41.0
15	38.7402628630397	41.0	38.0	41.0	35.0	41.0
16	38.79561627762647	41.0	38.0	41.0	35.0	41.0
17	38.71375002017699	40.0	38.0	41.0	35.0	41.0
18	38.7053814397298	40.0	38.0	41.0	35.0	41.0
19	38.669771974968285	40.0	37.0	41.0	35.0	41.0
20	38.61196615163857	40.0	37.0	41.0	35.0	41.0
21	38.5515843528975	40.0	37.0	41.0	35.0	41.0
22	38.65533081635822	40.0	37.0	41.0	35.0	41.0
23	38.66947363382298	40.0	37.0	41.0	35.0	41.0
24	38.6186370819119	40.0	37.0	41.0	35.0	41.0
25	38.52558915418616	40.0	37.0	41.0	35.0	41.0
26	38.411987414011236	40.0	36.0	41.0	35.0	41.0
27	38.336389103646276	40.0	36.0	41.0	35.0	41.0
28	38.255088915123615	40.0	36.0	41.0	34.0	41.0
29	38.222982175786385	40.0	36.0	41.0	34.0	41.0
30	38.12067064417753	40.0	36.0	41.0	34.0	41.0
31	38.07498548648253	40.0	35.0	41.0	34.0	41.0
32	37.952493068856135	40.0	35.0	41.0	34.0	41.0
33	37.870029572509424	40.0	35.0	41.0	34.0	41.0
34	37.77276423646653	40.0	35.0	41.0	34.0	41.0
35	37.68510203434151	40.0	35.0	41.0	33.0	41.0
36	37.664434117536395	40.0	35.0	41.0	33.0	41.0
37	37.556875455373785	40.0	35.0	41.0	33.0	41.0
38	37.47165174682637	40.0	35.0	41.0	33.0	41.0
39	37.352517893511134	39.0	35.0	41.0	33.0	41.0
40	37.218066782774805	39.0	35.0	41.0	33.0	41.0
41	37.13477282935944	39.0	35.0	41.0	33.0	41.0
42	37.09641762416919	39.0	35.0	41.0	33.0	41.0
43	36.99200323277122	39.0	35.0	41.0	33.0	41.0
44	36.85866589187026	39.0	35.0	41.0	33.0	41.0
45	36.74486989598692	38.0	35.0	41.0	32.0	41.0
46	36.71001574640112	38.0	35.0	41.0	32.0	41.0
47	36.66157983881786	38.0	35.0	41.0	32.0	41.0
48	36.5366077387244	38.0	35.0	40.0	32.0	41.0
49	36.448255901004174	38.0	35.0	40.0	32.0	41.0
50	36.33297821831336	37.0	35.0	40.0	32.0	41.0
51	36.220636635513394	37.0	35.0	40.0	32.0	41.0
52	35.903753810668036	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	2.0
11	5.0
12	7.0
13	16.0
14	43.0
15	56.0
16	97.0
17	157.0
18	311.0
19	572.0
20	996.0
21	1497.0
22	2195.0
23	3053.0
24	4191.0
25	5404.0
26	7208.0
27	8952.0
28	11714.0
29	14884.0
30	18635.0
31	23944.0
32	31925.0
33	44936.0
34	106076.0
35	201346.0
36	115589.0
37	164552.0
38	279307.0
39	747798.0
40	1127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.387438835890666	13.851266920145319	46.488953306827725	12.272340937136292
2	25.542176587901267	12.703265777988545	49.52629994083272	12.228257693277472
3	25.71650577952478	13.886166154866885	45.490067076663095	14.907260988945236
4	23.51829927735763	14.247626490244633	46.81200778581332	15.422066446584411
5	23.351372953705358	16.89729661733462	44.25506832067888	15.496262108281137
6	25.76070034470648	19.50160330535272	43.105564340663285	11.63213200927752
7	86.86541975652914	2.5814858168285557	6.224253465293629	4.32884096134868
8	87.57225449612908	2.9143365722272225	6.308579367372054	3.204829564271644
9	79.70072375558068	4.760099766169561	8.53444921827384	7.004727259975921
10	46.339114806236886	20.506055601661135	13.896908662524401	19.257920929577573
11	35.597720361950145	22.29432133233812	22.328329996476683	19.77962830923505
12	30.37235312682115	22.71416970156423	25.447330820811075	21.466146350803545
13	24.43057751832488	22.98935601171323	32.22373804756871	20.356328422393176
14	19.031882983478248	30.752738087087785	30.34279731559762	19.87258161383635
15	19.613314252858594	22.51768756668843	36.77494335136182	21.094054829091156
16	24.207990533234703	20.692797120785304	33.48495297509018	21.61425937088981
17	23.629453618249126	20.879204675940848	27.648042052742927	27.8432996530671
18	24.071733234034713	22.187174559070154	29.12783639773105	24.613255809164084
19	27.388218085150797	24.674204233438587	26.049356534923447	21.888221146487172
20	28.70592858403174	21.371578887020544	24.594553826920947	25.32793870202677
21	27.10574022835343	24.768883018544464	27.304782753655378	20.820593999446732
22	28.98000168095198	20.772224884657195	24.80511810914054	25.44265532525029
23	27.805060778659257	23.7545231245001	22.711219686508024	25.729196410332623
24	28.520578581443512	19.537894056610234	27.007833124884158	24.933694237062095
25	30.397010799838135	20.707435874743474	23.73025507611317	25.165298249305216
26	23.909872030573286	24.79893977572093	24.10451736362164	27.186670830084143
27	23.93402875763734	22.82710518362174	24.43402847933403	28.80483757940689
28	25.77255606559275	23.701144550181148	26.36417323601623	24.162126148209868
29	26.68004748967634	22.741109461700177	24.765821682165377	25.813021366458106
30	25.551638900345708	25.063828863503918	24.701811921511787	24.682720314638587
31	30.408031610802844	23.451506483632148	22.90836974932108	23.23209215624393
32	30.957124035887766	22.448501364521114	24.65578055450264	21.938594045088475
33	27.598782367370383	24.495366528238602	24.648266365208524	23.25758473918249
34	25.297714962865992	24.92913006282419	25.566333314965316	24.206821659344506
35	24.923118711388895	22.73309432645312	29.313743006933652	23.03004395522434
36	27.41883144894164	25.4046390934882	24.127226913488304	23.04930254408185
37	27.319254525629226	29.080914460138896	22.942712377428265	20.65711863680361
38	26.050469748152207	25.277231839456842	26.451170849843674	22.221127562547277
39	25.283076208907822	25.39996359792742	24.833783349781058	24.4831768433837
40	26.936364835597885	21.657952990118563	28.017851487336365	23.387830686947186
41	22.338849861488445	23.937758021953677	27.23476164156649	26.488630474991385
42	22.991526777509307	25.068337377080386	24.242555803987642	27.697580041422665
43	23.75708351492624	21.2772340658833	26.645259576277645	28.320422842912812
44	27.79092297065403	21.41710930807675	24.592271739801994	26.199695981467226
45	23.422228975715807	22.530879143449216	25.87686414512738	28.170027735707592
46	23.454178195381168	23.81630645869617	24.702591170771917	28.026924175150743
47	24.42367559630658	23.290480190092293	28.587760999799066	23.69808321380206
48	23.497593511302732	21.58130825931857	31.25474159259624	23.666356636782456
49	23.600231770994228	21.892673999402206	26.85704839304887	27.650045836554693
50	22.256472082560347	22.871355409464872	29.347640349749334	25.524532158225448
51	23.925290033791587	23.80623187897591	27.029039836892	25.239438250340505
52	23.219011900806024	22.53249330263091	27.407977619961247	26.840517176601818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	190.0
1	136.0
2	82.0
3	91.0
4	100.0
5	162.5
6	225.0
7	310.0
8	395.0
9	665.0
10	935.0
11	1263.0
12	1591.0
13	1505.5
14	1593.5
15	1767.0
16	1650.5
17	1534.0
18	1604.0
19	1674.0
20	2268.5
21	2863.0
22	2879.5
23	2896.0
24	3568.0
25	4240.0
26	6596.5
27	8953.0
28	10020.5
29	11088.0
30	12760.5
31	14433.0
32	17264.5
33	20096.0
34	22131.0
35	24166.0
36	28236.0
37	32306.0
38	34209.5
39	48163.5
40	60214.0
41	71041.5
42	81869.0
43	89682.0
44	97495.0
45	109337.5
46	121180.0
47	145123.0
48	169066.0
49	178055.0
50	187044.0
51	170430.5
52	153817.0
53	138721.0
54	123625.0
55	120395.5
56	117166.0
57	112806.5
58	108447.0
59	106859.5
60	105272.0
61	98037.0
62	90802.0
63	77043.5
64	57180.5
65	51076.0
66	42309.0
67	33542.0
68	28488.5
69	23435.0
70	19112.5
71	14790.0
72	12992.5
73	11195.0
74	9426.0
75	7657.0
76	5848.5
77	4040.0
78	3041.5
79	2043.0
80	1600.5
81	1158.0
82	906.0
83	654.0
84	481.5
85	309.0
86	251.0
87	193.0
88	120.5
89	33.5
90	19.0
91	18.0
92	17.0
93	13.0
94	9.0
95	7.5
96	6.0
97	8.5
98	11.0
99	30.5
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1796601.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.065529920714752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.9315363098847	11.763531953771729
2	12.089085047330853	4.126132851785799
3	5.162329202021661	2.642936503730405
4	2.7534586792506013	1.8795692590481143
5	1.6666569492403207	1.422119201741393
6	1.0705530079104206	1.0961732632923875
7	0.7862699191196542	0.9392678981346096
8	0.5597056127298439	0.7641338304666506
9	0.4272866705022601	0.6562686114161014
>10	3.418126571947929	13.252958071900423
>50	1.5466186277990153	19.45429519544982
>100	1.5772818648471094	38.537454681207144
>500	0.005871990396494887	0.6509107090568299
>1k	0.004240881953024085	1.869614637551789
>5k	9.786650660824812E-4	0.944633331446826
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT	6404	0.356450875848338	Illumina Paired End PCR Primer 2 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	5388	0.29989964382742745	TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	5176	0.28809958360259175	TruSeq Adapter, Index 22 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	4948	0.27540895279474964	No Hit
GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	4201	0.23383043870063525	TruSeq Adapter, Index 22 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3869	0.2153510991032511	No Hit
ATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3703	0.206111429304559	No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3491	0.19431136907972332	TruSeq Adapter, Index 22 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	2689	0.1496715186065242	No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	2047	0.11393737396338975	No Hit
TCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1939	0.1079260225280961	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	1930	0.10742507657515497	TruSeq Adapter, Index 22 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.566066143790413E-5	0.0	0.0	0.37353869890977465	0.0
2	5.566066143790413E-5	0.0	0.0	0.8779912735214997	0.0
3	5.566066143790413E-5	0.0	0.0	3.0629505382664264	0.0
4	5.566066143790413E-5	0.0	0.0	3.5227632624049523	0.0
5	5.566066143790413E-5	0.0	0.0	4.35088258327809	0.0
6	5.566066143790413E-5	0.0	0.0	6.8067979479027345	0.0
7	5.566066143790413E-5	0.0	0.0	7.112430639858266	0.0
8	5.566066143790413E-5	0.0	0.0	7.68968735963077	0.0
9	5.566066143790413E-5	0.0	0.0	8.0760836713327	0.0
10	5.566066143790413E-5	0.0	0.0	9.432311347928671	0.0
11	5.566066143790413E-5	0.0	0.0	10.50556022177434	0.0
12	5.566066143790413E-5	0.0	0.0	10.945502089779534	0.0
13	5.566066143790413E-5	0.0	0.0	11.161409795497164	0.0
14	5.566066143790413E-5	0.0	0.0	11.299392575201729	0.0
15	5.566066143790413E-5	0.0	0.0	11.508231376916745	0.0
16	5.566066143790413E-5	0.0	0.0	12.009789597133699	0.0
17	5.566066143790413E-5	0.0	0.0	12.616490806806853	0.0
18	1.1132132287580826E-4	0.0	0.0	13.300560335878695	0.0
19	1.1132132287580826E-4	0.0	0.0	13.753805101967549	0.0
20	1.1132132287580826E-4	0.0	0.0	14.287757827141363	0.0
21	1.1132132287580826E-4	0.0	0.0	15.027488017651109	0.0
22	1.669819843137124E-4	0.0	0.0	15.627509947951715	0.0
23	1.669819843137124E-4	0.0	0.0	16.263099040911143	0.0
24	2.7830330718952066E-4	0.0	0.0	16.794936660950317	0.0
25	2.7830330718952066E-4	0.0	0.0	17.233264369773813	0.0
26	2.7830330718952066E-4	0.0	0.0	17.639865501577702	0.0
27	2.7830330718952066E-4	0.0	0.0	18.00661359979205	0.0
28	2.7830330718952066E-4	0.0	0.0	18.39623822985738	0.0
29	2.7830330718952066E-4	0.0	0.0	18.798497830069113	0.0
30	2.7830330718952066E-4	0.0	0.0	19.23543402235666	0.0
31	2.7830330718952066E-4	0.0	0.0	19.656562586795843	0.0
32	2.7830330718952066E-4	0.0	0.0	20.076522277344832	0.0
33	2.7830330718952066E-4	0.0	0.0	20.493309310191858	0.0
34	2.7830330718952066E-4	0.0	0.0	20.898852889428426	0.0
35	2.7830330718952066E-4	0.0	0.0	21.341410808521204	0.0
36	2.7830330718952066E-4	0.0	0.0	21.764431835449273	0.0
37	2.7830330718952066E-4	0.0	0.0	22.186117006502837	0.0
38	2.7830330718952066E-4	0.0	0.0	22.62873058625705	0.0
39	2.7830330718952066E-4	0.0	0.0	23.129954842505377	0.0
40	2.7830330718952066E-4	0.0	0.0	23.61715261207135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	35	1.0199983E-7	46.000004	46
GACGTAT	35	1.0199983E-7	46.000004	35
GCTATAA	35	1.0199983E-7	46.000004	40
CTCGATT	35	1.0199983E-7	46.000004	42
AACGTAG	20	6.3124084E-4	46.0	37
ATAATCG	20	6.3124084E-4	46.0	16
TAGCGTA	25	3.4179295E-5	46.0	24
CGCGATA	20	6.3124084E-4	46.0	1
TAACGCG	25	3.4179295E-5	46.0	34
ACCTATC	20	6.3124084E-4	46.0	39
ACGATAG	25	3.4179295E-5	46.0	1
CGGATTA	25	3.4179295E-5	46.0	22
TAACACG	40	5.6152203E-9	46.0	25
ACTACGT	25	3.4179295E-5	46.0	38
GTCGTAC	30	1.8619921E-6	46.0	33
CGAATGT	30	1.8619921E-6	46.0	18
TCGTATT	25	3.4179295E-5	46.0	14
CTATTCG	20	6.3124084E-4	46.0	46
CGTCGAT	25	3.4179295E-5	46.0	30
ACCGTTC	25	3.4179295E-5	46.0	15
>>END_MODULE