FastQCFastQC Report
Thu 26 May 2016
SRR1545678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545678_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences757430
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC45200.5967548156265265No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.33032755502158617No Hit
ATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG21370.2821382834057273No Hit
ATGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT17680.23342091018311922No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT17170.22668761469706772No Hit
ATGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC15010.19817012793261424No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC13720.1811388511149545No Hit
ATGTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC13250.17493365723565213No Hit
ATTGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC12760.16846441255297517No Hit
ATATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC10870.14351161163407838No Hit
ATCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT10670.1408711036003327No Hit
ATGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC9940.13123324927716093No Hit
ATATTTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT9320.12304767437254928No Hit
ATTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC9000.11882286151855617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTC650.045.00000428
AGGTAAC453.8380676E-1045.00000433
AACCGAG453.8380676E-1045.0000043
CCTTACG453.8380676E-1045.00000431
GACACGG650.045.00000416
TGAACGT453.8380676E-1045.00000445
CACGAAT453.8380676E-1045.00000411
AGGCACC453.8380676E-1045.00000426
TATTCGA650.045.00000428
GTCGTTC650.045.00000429
GAGCCGT650.045.00000427
CGAGCCG650.045.00000426
ATGCGTC650.045.0000041
GCGTGTT650.045.00000410
GAACCTC453.8380676E-1045.00000412
ATTGCTA1550.045.0000041
TCCTTAC453.8380676E-1045.00000430
GTGCGTT453.8380676E-1045.0000043
TGCGTTA1250.045.0000042
CGAAGTA453.8380676E-1045.00000425