##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545678_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	757430
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.007458114941315	31.0	31.0	34.0	31.0	34.0
2	32.69663203200296	34.0	31.0	34.0	31.0	34.0
3	32.644177019658585	34.0	31.0	34.0	31.0	34.0
4	36.077285029639704	37.0	35.0	37.0	35.0	37.0
5	35.85173151314313	37.0	35.0	37.0	35.0	37.0
6	35.864930092549805	37.0	35.0	37.0	35.0	37.0
7	35.85819151604769	37.0	35.0	37.0	35.0	37.0
8	35.82318762129834	37.0	35.0	37.0	35.0	37.0
9	38.017591064520815	39.0	39.0	39.0	35.0	39.0
10	38.00901601468122	39.0	39.0	39.0	35.0	39.0
11	37.85191370819746	39.0	38.0	39.0	35.0	39.0
12	37.385787465508365	39.0	37.0	39.0	34.0	39.0
13	37.11300714257423	39.0	37.0	39.0	33.0	39.0
14	37.28774408196137	39.0	35.0	41.0	33.0	41.0
15	36.88769259205471	39.0	35.0	41.0	31.0	41.0
16	37.020139154773375	39.0	35.0	41.0	32.0	41.0
17	37.2210250452187	39.0	35.0	41.0	32.0	41.0
18	37.3956259984421	39.0	35.0	41.0	33.0	41.0
19	37.37658397475674	39.0	35.0	41.0	33.0	41.0
20	37.376444027830956	39.0	35.0	41.0	33.0	41.0
21	37.33650634382055	39.0	35.0	41.0	33.0	41.0
22	37.22102108445665	39.0	35.0	41.0	33.0	41.0
23	37.13562045337523	38.0	35.0	41.0	33.0	41.0
24	37.08384801235758	38.0	35.0	41.0	33.0	41.0
25	37.027614433016915	38.0	35.0	41.0	32.0	41.0
26	36.960000264050805	38.0	35.0	41.0	32.0	41.0
27	36.87888385725414	38.0	35.0	41.0	32.0	41.0
28	36.777616413397936	38.0	35.0	41.0	32.0	41.0
29	36.68258452926343	38.0	35.0	41.0	32.0	41.0
30	36.622045601573745	38.0	35.0	41.0	32.0	41.0
31	36.63329812655955	38.0	35.0	41.0	32.0	41.0
32	36.52660443869401	38.0	35.0	41.0	31.0	41.0
33	36.33272381606221	38.0	35.0	40.0	31.0	41.0
34	36.248824313797975	38.0	35.0	40.0	31.0	41.0
35	36.14092919477707	38.0	35.0	40.0	31.0	41.0
36	36.015874734298876	37.0	35.0	40.0	30.0	41.0
37	35.95497801777062	37.0	35.0	40.0	30.0	41.0
38	35.87816563906895	37.0	35.0	40.0	30.0	41.0
39	35.79548737177033	37.0	35.0	40.0	30.0	41.0
40	35.72895053008199	37.0	35.0	40.0	30.0	41.0
41	35.68502963970268	37.0	35.0	40.0	30.0	41.0
42	35.51720423009387	36.0	35.0	40.0	29.0	41.0
43	35.509868898776126	36.0	35.0	40.0	30.0	41.0
44	35.46248762261859	36.0	34.0	40.0	30.0	41.0
45	35.40026141029534	36.0	34.0	40.0	30.0	41.0
46	35.35270322009955	36.0	34.0	40.0	30.0	41.0
47	35.2374648482368	36.0	34.0	40.0	29.0	41.0
48	35.19953395033205	35.0	34.0	40.0	29.0	41.0
49	35.20402281398941	35.0	34.0	40.0	29.0	41.0
50	35.159498567524395	35.0	34.0	40.0	29.0	41.0
51	35.052773193562444	36.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	3.0
12	11.0
13	6.0
14	9.0
15	14.0
16	30.0
17	86.0
18	158.0
19	318.0
20	559.0
21	983.0
22	1440.0
23	2332.0
24	3476.0
25	4851.0
26	6853.0
27	8619.0
28	9832.0
29	11621.0
30	14489.0
31	18280.0
32	23299.0
33	31698.0
34	56000.0
35	140922.0
36	52007.0
37	68943.0
38	100804.0
39	199687.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.399865334090279	92.46002930963918	2.8348494250293754	3.305255931241171
2	2.833529171012503	1.7873598880424595	92.60895396274243	2.770156978202606
3	27.413358330142717	16.07660113805896	43.25548235480506	13.254558176993253
4	26.394518305321945	16.123470155657948	43.81210144831866	13.669910090701451
5	23.086225789841965	14.12896241236814	47.42431643848277	15.36049535930713
6	21.430759278085105	14.909760637946743	48.86220508825898	14.797274995709175
7	21.02095243124777	17.166206778184122	47.36437690611673	14.448463884451368
8	22.935716831918462	19.316240444661553	45.596028675917246	12.152014047502739
9	83.36902420025612	3.357141914104274	9.217353418797778	4.056480466841821
10	85.65834466551364	3.04080905166154	8.175144897878353	3.125701384946464
11	80.23592939281518	4.966531560672274	9.43691166180373	5.360627384708818
12	51.216614076548325	18.04166721677251	13.907819864541937	16.833898842137227
13	42.93796126374714	19.562467818808337	21.17911886246914	16.320452054975377
14	35.0108920956392	23.52349392023025	23.49048756980843	17.975126414322116
15	18.599342513499597	35.97639385817831	30.732080852355992	14.692182775966096
16	14.568739025388485	40.04739711920573	29.315052216046368	16.068811639359414
17	12.364046842612519	22.753257726786632	48.15349273200164	16.72920269859921
18	16.655532524457705	18.386781616783068	44.52147393158444	20.43621192717479
19	16.32414876622262	19.22791545093276	28.76463831641208	35.68329746643254
20	20.989266334842824	20.409014694427206	34.50549885798028	24.09622011274969
21	26.934502198222937	21.711313256670582	27.90858561187172	23.445598933234756
22	27.825805685013798	21.49756413133887	27.103362686980976	23.573267496666357
23	23.115403403614856	26.452345431260976	29.911939057074584	20.520312108049588
24	27.366753363347108	21.309031857729426	23.958253567986482	27.365961210936984
25	23.5011816273451	26.906776863868608	23.53616835879223	26.055873149994056
26	23.64614551839774	21.104524510515823	35.24193654859195	20.007393422494488
27	24.29333403746881	22.339226067095307	32.82151485945896	20.54592503597692
28	20.512258558546666	29.637986348573463	26.582126401119577	23.267628691760294
29	19.940060467633973	29.69185271246188	27.607039594417966	22.761047225486184
30	18.155605138428633	27.155380695245768	35.242200599395325	19.446813566930278
31	19.320465257515547	22.465442351108354	33.77487028504284	24.439222106333258
32	20.795717095969266	27.603342883170722	29.92408539402981	21.676854626830202
33	25.878034933921285	26.042934660628703	27.151023856990086	20.928006548459923
34	30.321613878510224	23.980829911675006	25.467964036280584	20.22959217353419
35	28.986573016648403	24.62973476096801	23.419986005307422	22.963706217076165
36	21.620743831113106	26.10089381196942	27.851154562137754	24.427207794779715
37	20.25177244101765	25.7242253409556	31.5782316517698	22.445770566256947
38	30.125292106201236	22.798938515770434	25.088919108036382	21.986850269991947
39	25.41198526596517	29.781233909404172	25.688182406294974	19.11859841833569
40	23.216930937512377	28.349022351900505	25.551536115548636	22.882510595038486
41	22.24258347306022	29.341457296383822	24.830413371532682	23.585545859023277
42	26.39953527058606	22.931227968261094	25.89876292198619	24.770473839166655
43	19.942172874060972	23.666873506462643	26.78782197694837	29.603131642528023
44	22.386227110095984	23.625021454127772	24.87041706824393	29.118334367532313
45	21.734153651162483	22.058408037706453	27.787914394729547	28.419523916401516
46	22.904822887923636	25.079413279114902	28.554057800720862	23.461706032240603
47	19.255772810688775	30.843378265978373	26.697780652997636	23.203068270335212
48	21.37847721901694	27.671864066646425	26.839311883606403	24.110346830730233
49	22.412632190433442	23.81566613416421	29.07265357854851	24.699048096853833
50	23.149597982651862	21.618367373882734	32.21617839272276	23.01585625074264
51	22.05141069141703	20.73564553820155	30.7600702375137	26.45287353286772
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	567.0
1	640.5
2	714.0
3	2170.0
4	3626.0
5	2533.0
6	1440.0
7	1492.5
8	1545.0
9	1706.5
10	1868.0
11	1915.0
12	1962.0
13	1976.0
14	1990.0
15	1792.5
16	1595.0
17	1565.5
18	1536.0
19	2033.5
20	2531.0
21	2614.5
22	2698.0
23	2977.5
24	3257.0
25	2862.5
26	3364.5
27	4261.0
28	5378.0
29	6495.0
30	7445.5
31	8396.0
32	11112.5
33	13829.0
34	13148.0
35	12467.0
36	16998.0
37	21529.0
38	31292.5
39	41056.0
40	54561.0
41	68066.0
42	74787.5
43	81509.0
44	86978.5
45	92448.0
46	86861.5
47	81275.0
48	73197.5
49	65120.0
50	55615.0
51	46110.0
52	41640.5
53	37171.0
54	34791.5
55	32412.0
56	31828.0
57	31244.0
58	29287.0
59	27330.0
60	24383.0
61	21436.0
62	18445.5
63	15455.0
64	11847.0
65	8239.0
66	6935.5
67	5632.0
68	4360.5
69	3089.0
70	2553.5
71	2018.0
72	1895.5
73	1773.0
74	1212.5
75	482.0
76	312.0
77	196.5
78	81.0
79	143.5
80	206.0
81	109.5
82	13.0
83	11.0
84	9.0
85	4.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	757430.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.10767179936517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.43139556522908	11.667011477347039
2	11.648578311789663	3.752629527510222
3	4.797091932167984	2.318099473042332
4	2.4392713252351594	1.5716392774596195
5	1.4539104074918066	1.1709555834779646
6	0.9033455428808316	0.8730476115686274
7	0.6530096508932607	0.736292559688467
8	0.44298192317126645	0.5708325945195485
9	0.34125747708185916	0.49471770959225714
>10	2.308404500355636	7.896040355690674
>50	0.7522412061398831	8.820726495545326
>100	1.805542960429534	55.94524803238598
>500	0.013945583974239926	1.504102010318215
>1k	0.009023613159802305	2.6786572918537166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	4520	0.5967548156265265	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2502	0.33032755502158617	No Hit
ATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	2137	0.2821382834057273	No Hit
ATGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	1768	0.23342091018311922	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	1717	0.22668761469706772	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	1501	0.19817012793261424	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1372	0.1811388511149545	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	1325	0.17493365723565213	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	1276	0.16846441255297517	No Hit
ATATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	1087	0.14351161163407838	No Hit
ATCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	1067	0.1408711036003327	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	994	0.13123324927716093	No Hit
ATATTTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	932	0.12304767437254928	No Hit
ATTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	900	0.11882286151855617	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3202540168728464E-4	0.0	0.0	0.2397581294641089	0.0
2	1.3202540168728464E-4	0.0	0.0	1.0959428594061498	0.0
3	1.3202540168728464E-4	0.0	0.0	1.496375902723684	0.0
4	1.3202540168728464E-4	0.0	0.0	2.0221010522424514	0.0
5	1.3202540168728464E-4	0.0	0.0	3.7087255587975125	0.0
6	1.3202540168728464E-4	0.0	0.0	4.069286930805487	0.0
7	1.3202540168728464E-4	0.0	0.0	4.632111218198381	0.0
8	1.3202540168728464E-4	0.0	0.0	6.481655070435552	0.0
9	1.3202540168728464E-4	0.0	0.0	6.71124724396974	0.0
10	1.3202540168728464E-4	0.0	0.0	7.151948034801896	0.0
11	1.3202540168728464E-4	0.0	0.0	7.475278243534056	0.0
12	1.3202540168728464E-4	0.0	0.0	8.353379190156186	0.0
13	2.640508033745693E-4	0.0	0.0	9.168636045575168	0.0
14	2.640508033745693E-4	0.0	0.0	9.51480664879923	0.0
15	3.960762050618539E-4	0.0	0.0	9.69079650924838	0.0
16	3.960762050618539E-4	0.0	0.0	9.774104537713056	0.0
17	3.960762050618539E-4	0.0	0.0	9.940984645445784	0.0
18	3.960762050618539E-4	0.0	0.0	10.327951097791216	0.0
19	3.960762050618539E-4	0.0	0.0	10.7818544287921	0.0
20	3.960762050618539E-4	0.0	0.0	11.365934805856647	0.0
21	3.960762050618539E-4	0.0	0.0	11.676590576026827	0.0
22	3.960762050618539E-4	0.0	0.0	12.034115363795994	0.0
23	3.960762050618539E-4	0.0	0.0	12.547430125556158	0.0
24	3.960762050618539E-4	0.0	0.0	12.933868476294839	0.0
25	5.281016067491386E-4	0.0	0.0	13.421306259324293	0.0
26	7.921524101237078E-4	0.0	0.0	13.743052163236207	0.0
27	7.921524101237078E-4	0.0	0.0	14.04776679033046	0.0
28	7.921524101237078E-4	0.0	0.0	14.35208534121965	0.0
29	7.921524101237078E-4	0.0	0.0	14.600425121793434	0.0
30	7.921524101237078E-4	0.0	0.0	14.871869347662491	0.0
31	7.921524101237078E-4	0.0	0.0	15.160080799545833	0.0
32	7.921524101237078E-4	0.0	0.0	15.480374424039185	0.0
33	7.921524101237078E-4	0.0	0.0	15.828657433690243	0.0
34	7.921524101237078E-4	0.0	0.0	16.107231031250414	0.0
35	7.921524101237078E-4	0.0	0.0	16.426072376325205	0.0
36	7.921524101237078E-4	0.0	0.0	16.72471383494184	0.0
37	7.921524101237078E-4	0.0	0.0	17.102438509169165	0.0
38	7.921524101237078E-4	0.0	0.0	17.42986150535363	0.0
39	7.921524101237078E-4	0.0	0.0	17.760057034973528	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGTC	65	0.0	45.000004	28
AGGTAAC	45	3.8380676E-10	45.000004	33
AACCGAG	45	3.8380676E-10	45.000004	3
CCTTACG	45	3.8380676E-10	45.000004	31
GACACGG	65	0.0	45.000004	16
TGAACGT	45	3.8380676E-10	45.000004	45
CACGAAT	45	3.8380676E-10	45.000004	11
AGGCACC	45	3.8380676E-10	45.000004	26
TATTCGA	65	0.0	45.000004	28
GTCGTTC	65	0.0	45.000004	29
GAGCCGT	65	0.0	45.000004	27
CGAGCCG	65	0.0	45.000004	26
ATGCGTC	65	0.0	45.000004	1
GCGTGTT	65	0.0	45.000004	10
GAACCTC	45	3.8380676E-10	45.000004	12
ATTGCTA	155	0.0	45.000004	1
TCCTTAC	45	3.8380676E-10	45.000004	30
GTGCGTT	45	3.8380676E-10	45.000004	3
TGCGTTA	125	0.0	45.000004	2
CGAAGTA	45	3.8380676E-10	45.000004	25
>>END_MODULE