FastQCFastQC Report
Thu 26 May 2016
SRR1545677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545677_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences622888
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT35600.571531318631921Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC25530.4098650158615995No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT25360.4071357932726269No Hit
TTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC22150.35560164909261377No Hit
TGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC21920.351909171472239No Hit
GCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC21620.34709289631522844No Hit
GGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC16760.26906923877165717No Hit
ATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC15700.2520517332168865No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT15000.24081375785052853No Hit
TCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC11400.18301845596640165No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC9510.15267592247723508No Hit
ACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC9500.1525153799720014No Hit
CTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC7810.1253836965875085No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT6850.10997161608507469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGT1450.046.00000441
TACGGGA1450.046.0000044
TGATCGG253.4153407E-546.02
AGGTCAG206.309216E-446.08
CGGACAG206.309216E-446.010
ACGCGAA206.309216E-446.046
CTAGATT301.8600203E-646.016
GTCATCG206.309216E-446.021
CAGCGTG206.309216E-446.031
AACCGTT253.4153407E-546.036
CTAGACT253.4153407E-546.037
CGGGTAC206.309216E-446.06
AATCCTC253.4153407E-546.023
CTGTCTA206.309216E-446.033
ACTATGA206.309216E-446.08
CGGAACA301.8600203E-646.016
AGGTAAC206.309216E-446.031
ACTATCA301.8600203E-646.015
CTCACTA301.8600203E-646.045
CTCACGA253.4153407E-546.014
Produced by FastQC (version 0.11.5)