##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545677_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	622888
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.670921578197046	33.0	31.0	34.0	31.0	34.0
2	33.0462667445833	34.0	33.0	34.0	31.0	34.0
3	33.09536866980902	34.0	33.0	34.0	31.0	34.0
4	36.460638188566804	37.0	37.0	37.0	35.0	37.0
5	36.45205879708712	37.0	37.0	37.0	35.0	37.0
6	36.460492094887044	37.0	37.0	37.0	35.0	37.0
7	36.530181990983934	37.0	37.0	37.0	35.0	37.0
8	36.58840915220714	37.0	37.0	37.0	35.0	37.0
9	38.43768061031839	39.0	39.0	39.0	37.0	39.0
10	38.19941305660087	39.0	39.0	39.0	37.0	39.0
11	38.0181043783152	39.0	39.0	39.0	35.0	39.0
12	37.28642548901247	39.0	35.0	39.0	35.0	39.0
13	37.07291198417693	39.0	35.0	39.0	34.0	39.0
14	38.31447868637701	40.0	35.0	41.0	35.0	41.0
15	38.38226133751172	40.0	35.0	41.0	35.0	41.0
16	38.51507815209155	40.0	35.0	41.0	35.0	41.0
17	38.49445807271933	40.0	35.0	41.0	35.0	41.0
18	38.52808048959042	40.0	36.0	41.0	35.0	41.0
19	38.49324276595471	40.0	36.0	41.0	35.0	41.0
20	38.38530201256084	40.0	35.0	41.0	35.0	41.0
21	38.33107878141817	40.0	35.0	41.0	35.0	41.0
22	38.40118929887877	40.0	35.0	41.0	35.0	41.0
23	38.3880858196016	40.0	35.0	41.0	35.0	41.0
24	38.35963929309924	40.0	35.0	41.0	35.0	41.0
25	38.27481344960892	40.0	35.0	41.0	35.0	41.0
26	38.16997758826627	40.0	35.0	41.0	35.0	41.0
27	38.083273397464716	40.0	35.0	41.0	35.0	41.0
28	38.06975892937414	40.0	35.0	41.0	35.0	41.0
29	38.09035492737057	40.0	35.0	41.0	35.0	41.0
30	38.001444882547105	40.0	35.0	41.0	35.0	41.0
31	37.84567209514391	40.0	35.0	41.0	35.0	41.0
32	37.803287268337165	40.0	35.0	41.0	34.0	41.0
33	37.74135318066811	40.0	35.0	41.0	34.0	41.0
34	37.59448247518013	39.0	35.0	41.0	34.0	41.0
35	37.60268298634747	39.0	35.0	41.0	34.0	41.0
36	37.548748731714205	39.0	35.0	41.0	34.0	41.0
37	37.45528730686737	39.0	35.0	41.0	34.0	41.0
38	37.37605315883433	39.0	35.0	41.0	34.0	41.0
39	37.320847407559626	39.0	35.0	41.0	34.0	41.0
40	37.169937452639964	39.0	35.0	41.0	33.0	41.0
41	37.14121318760355	38.0	35.0	41.0	33.0	41.0
42	37.12348126790049	38.0	35.0	41.0	34.0	41.0
43	37.026815414649185	38.0	35.0	41.0	33.0	41.0
44	36.918786362877434	38.0	35.0	41.0	33.0	41.0
45	36.838694917866455	38.0	35.0	40.0	33.0	41.0
46	36.83265370339451	38.0	35.0	41.0	33.0	41.0
47	36.83994233313212	38.0	35.0	41.0	33.0	41.0
48	36.78435609612001	38.0	35.0	40.0	33.0	41.0
49	36.76619552792798	37.0	35.0	40.0	33.0	41.0
50	36.66371803598721	37.0	35.0	40.0	33.0	41.0
51	36.55314278008245	37.0	35.0	40.0	33.0	41.0
52	36.219278586198485	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	1.0
15	17.0
16	11.0
17	22.0
18	46.0
19	99.0
20	180.0
21	321.0
22	553.0
23	835.0
24	1200.0
25	1646.0
26	2085.0
27	2512.0
28	3106.0
29	4113.0
30	5328.0
31	6998.0
32	9375.0
33	13892.0
34	37720.0
35	115540.0
36	35029.0
37	46473.0
38	78980.0
39	256416.0
40	387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.425957796586225	14.09948497964321	45.98499248661076	12.48956473715981
2	24.654191443726642	14.011828771785616	48.67632704434826	12.65765274013948
3	24.643274553370752	13.969766635414393	45.51797433888596	15.868984472328892
4	21.834422881802183	14.918091213829774	48.26180629583489	14.985679608533156
5	22.157594944837594	16.94863282002543	46.188881468257534	14.704890766879439
6	22.121793966170483	18.91800773172705	46.102188515431344	12.858009786671118
7	85.28932970293215	2.4635247428109066	7.149921013087425	5.09722454116952
8	87.68510550853445	2.355158551778169	6.570683654204287	3.3890522854831047
9	81.31638432591413	3.21373986976792	8.993430600685839	6.476445203632114
10	50.71393252077419	17.59417423357008	12.609811073579841	19.08208217207588
11	41.66463312826704	18.41567023285085	22.81678247132711	17.102914167555003
12	34.49544701455157	23.65224566856321	23.238367090070767	18.613940226814453
13	19.48713091278047	33.25381127907425	32.60168762281502	14.657370185330269
14	14.479649632036578	40.23773134175004	29.15548220546872	16.12713682074466
15	12.110844967313545	22.870243125569925	46.16335520992538	18.855556697191147
16	16.45785438152605	19.954149060505262	42.48661075506351	21.101385802905178
17	16.30004109888134	20.682369864245256	26.856995158038043	36.160593878835364
18	20.688309936938904	21.802153838250216	32.27947881481101	25.230057409999873
19	25.035640436161877	23.208506184097303	27.603357264869448	24.152496114871376
20	26.80337396128999	20.87277327545241	25.90738623958079	26.416466523676807
21	22.598765749219762	27.33075609098265	30.045529854484272	20.024948305313313
22	28.034092806411426	20.89589139620606	23.430536468835488	27.63947932854703
23	23.860790382861765	27.45276839496025	21.325182055200937	27.36125916697705
24	24.537637584926987	20.495016760637547	32.585954457302115	22.38139119713335
25	26.69308126019445	22.215069161711256	29.19385186421957	21.897997713874727
26	19.6679017736736	29.616239195489396	25.555958695624252	25.159900335212747
27	19.24631715492994	27.86247286831662	27.683467974981056	25.207742001772388
28	19.570773558007218	25.7120060107114	34.33458342430742	20.382637006973965
29	21.35985923633141	20.53868432206111	32.160195733422384	25.941260708185098
30	22.35425951374886	26.3220675305994	29.81017454181169	21.513498413840047
31	25.04061725382412	24.96548336137476	27.96120650903533	22.03269287576579
32	30.608552420338807	25.17418861817855	23.54949846521365	20.66776049626899
33	29.085485673186835	24.241115577760368	22.995626822157433	23.677771926895364
34	20.067652611705476	26.802571248763822	28.799559471365637	24.330216668165065
35	19.94836953031685	25.53524871244911	31.03848524935462	23.477896507879425
36	29.377994117722604	21.418457250741707	25.075615519965066	24.12793311157062
37	27.096845660857166	29.2673803316166	24.250427043063922	19.38534696446231
38	22.572276235856208	26.756174464751286	27.65392815401806	23.017621145374452
39	21.947123720476235	29.192246439167235	24.825169211800517	24.035460628556017
40	26.408760483425592	22.22277520196247	26.769981120201386	24.59848319441055
41	20.11581536327558	23.260200870782548	27.218376337319068	29.405607428622805
42	22.214587534195555	24.433124414019854	24.104333363301265	29.247954688483325
43	22.704723802673996	20.675145451509742	28.742727424512914	27.877403321303344
44	25.84044001489835	23.436476541529135	26.176134393341982	24.54694905023054
45	19.96249727077741	27.842405055162406	27.202964256816635	24.99213341724355
46	22.108468938236086	25.90305159193948	26.551964398094043	25.43651507173039
47	23.045716083790342	23.191809763553	29.49904316666881	24.26343098598785
48	22.304009709610717	20.426144025892295	34.25238566162777	23.017460602869217
49	22.698462644969883	18.98415124388333	29.645779016452394	28.67160709469439
50	19.595497103813205	25.709276788122427	29.81226159437973	24.882964513684644
51	21.94519721041343	25.529469182260694	26.77415522533746	25.751178381988417
52	20.342501380665546	22.859326235214038	29.189677759083494	27.608494625036922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	299.0
1	240.0
2	181.0
3	143.5
4	106.0
5	131.5
6	157.0
7	234.5
8	312.0
9	355.0
10	398.0
11	464.0
12	530.0
13	558.0
14	523.5
15	461.0
16	471.0
17	481.0
18	721.0
19	961.0
20	856.0
21	751.0
22	913.0
23	1075.0
24	1226.0
25	1377.0
26	1270.0
27	1163.0
28	1757.0
29	2351.0
30	3419.0
31	4487.0
32	5475.5
33	6464.0
34	7661.0
35	8858.0
36	8913.0
37	8968.0
38	12326.5
39	22446.5
40	29208.0
41	40734.0
42	52260.0
43	57776.0
44	63292.0
45	68789.5
46	74287.0
47	76472.5
48	78658.0
49	68718.5
50	58779.0
51	49743.5
52	40708.0
53	37409.5
54	34111.0
55	30503.5
56	26896.0
57	25828.5
58	24761.0
59	24330.0
60	23899.0
61	21065.0
62	18231.0
63	17082.5
64	12979.0
65	10024.0
66	7805.0
67	5586.0
68	4774.5
69	3963.0
70	3222.0
71	2481.0
72	2236.0
73	1991.0
74	1445.5
75	900.0
76	722.5
77	545.0
78	415.5
79	286.0
80	277.5
81	269.0
82	150.0
83	31.0
84	76.5
85	122.0
86	64.0
87	6.0
88	7.5
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	622888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.116695306327326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.03479450126433	10.642626625928164
2	13.091907884272741	4.219965806986565
3	5.998367562070655	2.900215869997507
4	3.2735472559766894	2.1103505478176086
5	1.9987214419010917	1.6106392240671557
6	1.380807511831475	1.335243236692551
7	0.8938753904417394	1.0084422116701723
8	0.7039018734320495	0.9075657615725861
9	0.5019300290660164	0.7280508009197705
>10	3.1925549458321276	10.825847909824965
>50	1.0978469560049091	13.039167465077877
>100	1.8117474355928012	46.204959306710414
>500	0.009998606156693162	1.066131938664235
>1k	0.009998606156693162	3.4007932940704375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT	3560	0.571531318631921	Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2553	0.4098650158615995	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	2536	0.4071357932726269	No Hit
TTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2215	0.35560164909261377	No Hit
TGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2192	0.351909171472239	No Hit
GCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2162	0.34709289631522844	No Hit
GGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1676	0.26906923877165717	No Hit
ATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1570	0.2520517332168865	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	1500	0.24081375785052853	No Hit
TCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1140	0.18301845596640165	No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	951	0.15267592247723508	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	950	0.1525153799720014	No Hit
CTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	781	0.1253836965875085	No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	685	0.10997161608507469	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5861406866081864	0.0
2	0.0	0.0	0.0	1.40041227315344	0.0
3	0.0	0.0	0.0	4.428243921860751	0.0
4	0.0	0.0	0.0	5.048419619578479	0.0
5	0.0	0.0	0.0	5.995620400457225	0.0
6	0.0	0.0	0.0	9.269563709687777	0.0
7	0.0	0.0	0.0	9.695322433567512	0.0
8	0.0	0.0	0.0	10.489686749463788	0.0
9	0.0	0.0	0.0	10.983997123078305	0.0
10	0.0	0.0	0.0	12.565661884640578	0.0
11	0.0	0.0	0.0	13.969445550403925	0.0
12	0.0	0.0	0.0	14.549967249328933	0.0
13	0.0	0.0	0.0	14.839585928770502	0.0
14	0.0	0.0	0.0	14.971230783062124	0.0
15	0.0	0.0	0.0	15.208191520787043	0.0
16	0.0	0.0	0.0	15.832220238630379	0.0
17	0.0	0.0	0.0	16.578582345461786	0.0
18	0.0	0.0	0.0	17.517113831057912	0.0
19	0.0	0.0	0.0	18.028281167721968	0.0
20	0.0	0.0	0.0	18.58905613850323	0.0
21	1.6054250523368568E-4	0.0	0.0	19.425964218286435	0.0
22	1.6054250523368568E-4	0.0	0.0	20.040199843310514	0.0
23	1.6054250523368568E-4	0.0	0.0	20.79876318053968	0.0
24	3.2108501046737136E-4	0.0	0.0	21.282477748808773	0.0
25	3.2108501046737136E-4	0.0	0.0	21.71225003531935	0.0
26	3.2108501046737136E-4	0.0	0.0	22.153902467217222	0.0
27	3.2108501046737136E-4	0.0	0.0	22.52925084445358	0.0
28	3.2108501046737136E-4	0.0	0.0	22.895127213881146	0.0
29	3.2108501046737136E-4	0.0	0.0	23.28508495909377	0.0
30	3.2108501046737136E-4	0.0	0.0	23.73412234623239	0.0
31	3.2108501046737136E-4	0.0	0.0	24.183480818381476	0.0
32	3.2108501046737136E-4	0.0	0.0	24.582107858876718	0.0
33	3.2108501046737136E-4	0.0	0.0	25.00176596755757	0.0
34	3.2108501046737136E-4	0.0	0.0	25.378719769846263	0.0
35	3.2108501046737136E-4	0.0	0.0	25.896308806719667	0.0
36	3.2108501046737136E-4	0.0	0.0	26.329131400829684	0.0
37	3.2108501046737136E-4	0.0	0.0	26.742528351806424	0.0
38	6.421700209347427E-4	0.0	0.0	27.18225427364149	0.0
39	6.421700209347427E-4	0.0	0.0	27.60383889238515	0.0
40	6.421700209347427E-4	0.0	0.0	28.043725356725446	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGT	145	0.0	46.000004	41
TACGGGA	145	0.0	46.000004	4
TGATCGG	25	3.4153407E-5	46.0	2
AGGTCAG	20	6.309216E-4	46.0	8
CGGACAG	20	6.309216E-4	46.0	10
ACGCGAA	20	6.309216E-4	46.0	46
CTAGATT	30	1.8600203E-6	46.0	16
GTCATCG	20	6.309216E-4	46.0	21
CAGCGTG	20	6.309216E-4	46.0	31
AACCGTT	25	3.4153407E-5	46.0	36
CTAGACT	25	3.4153407E-5	46.0	37
CGGGTAC	20	6.309216E-4	46.0	6
AATCCTC	25	3.4153407E-5	46.0	23
CTGTCTA	20	6.309216E-4	46.0	33
ACTATGA	20	6.309216E-4	46.0	8
CGGAACA	30	1.8600203E-6	46.0	16
AGGTAAC	20	6.309216E-4	46.0	31
ACTATCA	30	1.8600203E-6	46.0	15
CTCACTA	30	1.8600203E-6	46.0	45
CTCACGA	25	3.4153407E-5	46.0	14
>>END_MODULE