FastQCFastQC Report
Thu 26 May 2016
SRR1545675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545675_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2094663
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT105510.5037087111387369Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT68060.3249210016121925No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC64720.308975715902749No Hit
GTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC61750.294796824119202No Hit
TGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC60980.2911208151382824No Hit
TTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC54730.2612830799035453No Hit
ATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC44130.2106782809454313No Hit
GGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC38420.18341852603497555No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC32270.1540581945639943No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT28310.1351530055192649No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC25880.12355209405999915No Hit
TCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC22760.1086570966308184No Hit
CTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC20950.10001608850683857No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA405.6152203E-946.00000436
CGGAACC405.6152203E-946.00000437
AACCGAT405.6152203E-946.00000423
TCGCAAA206.3126476E-446.00000420
GCGAATC351.02010745E-746.00000418
CCCTATA206.3126476E-446.00000442
CTATCGT206.3126476E-446.00000422
GCAACGC405.6152203E-946.00000425
TAACGGA405.6152203E-946.00000429
CGACACG405.6152203E-946.0000041
TCGATAG405.6152203E-946.00000435
CCGTCGA405.6152203E-946.00000443
CCCGTTA206.3126476E-446.00000437
AATGCGT405.6152203E-946.00000441
TACGCTT351.02010745E-746.00000427
CTAACGG351.02010745E-746.0000042
CTAACGA405.6152203E-946.00000421
CGGATTA351.02010745E-746.00000426
CTCGGTT405.6152203E-946.00000421
TCTAACG206.3126476E-446.00000439