##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545675_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2094663
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.617495033807344	33.0	31.0	34.0	31.0	34.0
2	33.022347270181406	34.0	33.0	34.0	31.0	34.0
3	33.08947740042193	34.0	33.0	34.0	31.0	34.0
4	36.44667996713552	37.0	37.0	37.0	35.0	37.0
5	36.41957011700689	37.0	37.0	37.0	35.0	37.0
6	36.435332557074815	37.0	37.0	37.0	35.0	37.0
7	36.51792865964597	37.0	37.0	37.0	35.0	37.0
8	36.55974970675474	37.0	37.0	37.0	35.0	37.0
9	38.38602104491272	39.0	39.0	39.0	37.0	39.0
10	38.13735670129276	39.0	39.0	39.0	37.0	39.0
11	37.97654133385657	39.0	38.0	39.0	35.0	39.0
12	37.62331458568753	39.0	37.0	39.0	35.0	39.0
13	37.553195430482134	39.0	37.0	39.0	35.0	39.0
14	38.94533249501232	41.0	38.0	41.0	35.0	41.0
15	38.975999480584704	41.0	38.0	41.0	35.0	41.0
16	39.04108489050506	41.0	38.0	41.0	35.0	41.0
17	38.974118509755506	41.0	38.0	41.0	35.0	41.0
18	38.947651245092885	41.0	38.0	41.0	35.0	41.0
19	38.94367065251069	41.0	38.0	41.0	35.0	41.0
20	38.858940555115545	41.0	38.0	41.0	35.0	41.0
21	38.8174985665952	41.0	38.0	41.0	35.0	41.0
22	38.90258767162069	41.0	38.0	41.0	35.0	41.0
23	38.905873164322855	41.0	38.0	41.0	35.0	41.0
24	38.88721383821646	41.0	38.0	41.0	35.0	41.0
25	38.81679678306248	41.0	38.0	41.0	35.0	41.0
26	38.69995077967196	40.0	38.0	41.0	35.0	41.0
27	38.605707457476456	40.0	37.0	41.0	35.0	41.0
28	38.57993577009763	40.0	37.0	41.0	35.0	41.0
29	38.518725446527675	40.0	37.0	41.0	35.0	41.0
30	38.485184490297485	40.0	37.0	41.0	35.0	41.0
31	38.425126142009475	40.0	36.0	41.0	35.0	41.0
32	38.36099028817523	40.0	36.0	41.0	35.0	41.0
33	38.31837006716594	40.0	36.0	41.0	35.0	41.0
34	38.2152269840065	40.0	36.0	41.0	34.0	41.0
35	38.0755696739762	40.0	36.0	41.0	34.0	41.0
36	38.01869942802255	40.0	35.0	41.0	34.0	41.0
37	37.94923288376221	40.0	35.0	41.0	34.0	41.0
38	37.81402975084775	40.0	35.0	41.0	34.0	41.0
39	37.80879024454053	40.0	35.0	41.0	34.0	41.0
40	37.659227761219825	40.0	35.0	41.0	34.0	41.0
41	37.538188720572236	40.0	35.0	41.0	33.0	41.0
42	37.465985697938045	39.0	35.0	41.0	33.0	41.0
43	37.384593607659085	39.0	35.0	41.0	33.0	41.0
44	37.259142401426864	39.0	35.0	41.0	33.0	41.0
45	37.22756739389582	39.0	35.0	41.0	33.0	41.0
46	37.2353008574649	39.0	35.0	41.0	33.0	41.0
47	37.20906035959006	39.0	35.0	41.0	33.0	41.0
48	37.115598069952064	39.0	35.0	41.0	33.0	41.0
49	37.04136082988051	39.0	35.0	41.0	33.0	41.0
50	36.949012800627116	39.0	35.0	41.0	33.0	41.0
51	36.841108569731745	38.0	35.0	40.0	33.0	41.0
52	36.51753862077098	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	5.0
13	14.0
14	24.0
15	31.0
16	63.0
17	115.0
18	215.0
19	432.0
20	788.0
21	1429.0
22	1913.0
23	2662.0
24	3647.0
25	5080.0
26	6483.0
27	8428.0
28	10687.0
29	13996.0
30	17946.0
31	23610.0
32	31592.0
33	44417.0
34	101441.0
35	229761.0
36	120389.0
37	171177.0
38	299966.0
39	996166.0
40	2179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.12813469278829	13.467703396680038	47.13942051776348	12.264741392768192
2	25.32512389821179	13.04286178731376	49.23202443543425	12.39998987904021
3	25.400076289121447	13.765364643381774	45.64118428596867	15.193374781528101
4	23.49184570501317	13.78207377511323	47.51203415537487	15.21404636449873
5	23.127013748750993	16.89469857442462	44.90884691236729	15.069440764457099
6	24.63742377652157	19.226815960371667	44.10771565640869	12.028044606698069
7	86.38797744553659	2.7696579354292314	6.376538851356996	4.465825767677187
8	87.57098397212344	2.8306701364372215	6.521621855162382	3.0767240362769575
9	79.90798519857371	4.837914261148453	8.731141954576941	6.5229585857008985
10	42.63793268893373	25.083318891869478	13.565953091260981	18.712795327935805
11	33.24630262720065	22.249211448333217	26.06634098181903	18.4381449426471
12	29.277692879475126	23.532711467190666	26.63483338369943	20.554762269634782
13	21.994564280745877	26.06194886719248	32.29927678103829	19.64421007102336
14	16.924488569282982	33.523769694695524	30.08421879796416	19.46752293805734
15	17.39038690233226	23.89529962576319	38.39825308414767	20.316060387756885
16	20.466585794469086	22.14408713955419	34.951970794347346	22.437356271629376
17	19.939054635518936	22.035907446687126	28.402898222769007	29.62213969502493
18	21.587864014402317	23.194279939064185	30.62373279138458	24.59412325514892
19	24.81248773669082	25.63400413336179	27.41839618115181	22.135111948795583
20	27.723361705439014	21.68988519871693	26.834722339583983	23.752030756260076
21	25.132682441041826	25.62918235534785	27.640532152427383	21.597603051182933
22	27.97404642178718	20.815711166903696	25.45836728867603	25.751875122633095
23	25.85685621028299	24.88180676318816	22.99682574237479	26.264511284154064
24	25.95715874104808	20.438753155042125	29.605764745928102	23.99832335798169
25	26.955171309179565	23.05277746348697	25.39692542428066	24.595125803052806
26	22.11310363528644	27.223854147421328	25.40208138492922	25.26096083236301
27	22.0602072982623	24.93938165709711	27.76799895735018	25.232412087290413
28	24.859321045915262	24.40416429755049	29.281941772972548	21.4545728835617
29	21.836066231178954	22.77010669496716	30.28463289798884	25.109194175865046
30	22.780657318146165	25.476508631698753	28.201195132582185	23.5416389175729
31	25.40637801880302	28.129823269900694	23.852046844766914	22.611751866529364
32	24.915320507403816	26.414702508231635	26.151223371014815	22.518753613349737
33	25.441849118450083	27.095146092712763	22.70675521551677	24.756249573320385
34	23.07077558538056	26.82006604403668	26.530043257555036	23.57911511302773
35	22.582391535058385	25.86611784329985	27.71214271699075	23.839347904651014
36	25.964224316751665	26.82082989005869	24.48040567862229	22.734540114567356
37	26.498152686136144	26.098661216625302	25.46290262443171	21.940283472806843
38	21.35088078607394	26.78512008852975	27.932416813587675	23.93158231180863
39	23.486069119471725	27.778310878647304	24.364444304406007	24.371175697474964
40	25.480900746325304	24.6915136229551	27.406174644799663	22.42141098591993
41	21.98730774353679	24.61637027053994	28.199285517527162	25.19703646839611
42	22.692337621851344	25.431728158658455	25.22596713647971	26.64996708301049
43	23.22588406822482	21.4422558664568	28.583595547350576	26.748264517967808
44	25.101412494515824	23.216192771820573	25.951859559270396	25.73053517439321
45	20.508072181539465	25.35343394140251	27.59498783336508	26.54350604369295
46	21.684967939950244	26.450746492395194	25.51641958634874	26.347865981305823
47	22.88277398321353	24.455580682906987	29.828043938332797	22.833601395546683
48	22.273177117273757	21.736718507941372	31.8677992593558	24.122305115429068
49	21.630925833893087	22.76265919625257	27.128325654293796	28.478089315560545
50	20.515997084017812	24.332171809976117	30.664503072809325	24.487328033196746
51	21.725356298363984	25.674535712904657	27.039528554235215	25.560579434496145
52	22.102027867967305	22.719072232621667	28.80415608620575	26.374743813205274
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	388.0
1	295.5
2	203.0
3	193.5
4	184.0
5	251.5
6	319.0
7	431.5
8	544.0
9	918.0
10	1292.0
11	1243.5
12	1195.0
13	1491.5
14	1746.5
15	1705.0
16	2137.5
17	2570.0
18	2243.5
19	1917.0
20	2442.5
21	2968.0
22	4212.0
23	5456.0
24	6305.0
25	7154.0
26	10589.5
27	14025.0
28	16984.0
29	19943.0
30	20552.5
31	21162.0
32	26393.0
33	31624.0
34	37300.5
35	42977.0
36	48103.5
37	53230.0
38	60575.0
39	84278.5
40	100637.0
41	117912.5
42	135188.0
43	151359.0
44	167530.0
45	188020.0
46	208510.0
47	216940.5
48	225371.0
49	199193.0
50	173015.0
51	150289.0
52	127563.0
53	117236.5
54	106910.0
55	102454.0
56	97998.0
57	100012.5
58	102027.0
59	103826.5
60	105626.0
61	91663.0
62	77700.0
63	67584.0
64	52161.5
65	46855.0
66	37235.5
67	27616.0
68	23413.0
69	19210.0
70	16797.5
71	14385.0
72	12253.5
73	10122.0
74	8215.0
75	6308.0
76	4492.0
77	2676.0
78	2406.5
79	2137.0
80	1380.0
81	623.0
82	414.5
83	206.0
84	267.5
85	329.0
86	187.0
87	45.0
88	31.5
89	14.0
90	10.0
91	9.0
92	8.0
93	5.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2094663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.84259423290439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.1324528464542	9.016021161472441
2	12.48027864649252	3.455188664339544
3	5.829089607525074	2.4206916655262853
4	3.498579529821957	1.937176672914831
5	2.2427631398559225	1.5522830052770065
6	1.5574279817141667	1.2935306158704272
7	1.1236424756650865	1.0887888797441556
8	0.8272855482605951	0.9161422527453807
9	0.6614629052951695	0.8240726338307186
>10	3.862270610901201	10.31382249089745
>50	0.7880973008884669	8.004221337360722
>100	1.9738660632685343	54.361386754077856
>500	0.016224502443756905	1.4220797254979676
>1k	0.004487628335507228	1.4079353841422084
>5k	0.001726010898272011	1.4824798858820212
>10k+	3.4520217965440216E-4	0.5041788704210033
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT	10551	0.5037087111387369	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	6806	0.3249210016121925	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	6472	0.308975715902749	No Hit
GTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	6175	0.294796824119202	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	6098	0.2911208151382824	No Hit
TTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	5473	0.2612830799035453	No Hit
ATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	4413	0.2106782809454313	No Hit
GGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3842	0.18341852603497555	No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3227	0.1540581945639943	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	2831	0.1351530055192649	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2588	0.12355209405999915	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2276	0.1086570966308184	No Hit
CTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2095	0.10001608850683857	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5210862081394477	0.0
2	0.0	0.0	0.0	1.1113004812707343	0.0
3	0.0	0.0	0.0	3.4536343077621554	0.0
4	0.0	0.0	0.0	3.980974505206804	0.0
5	0.0	0.0	0.0	4.840587722225484	0.0
6	0.0	0.0	0.0	7.441817609801672	0.0
7	0.0	0.0	0.0	7.762871640927442	0.0
8	0.0	0.0	0.0	8.377003842622894	0.0
9	0.0	0.0	0.0	8.791056126928293	0.0
10	0.0	0.0	0.0	10.187271174408485	0.0
11	0.0	0.0	0.0	11.396678129131034	0.0
12	0.0	0.0	0.0	11.927646595180228	0.0
13	0.0	0.0	0.0	12.170549630179174	0.0
14	0.0	0.0	0.0	12.311479221239884	0.0
15	0.0	0.0	0.0	12.545263844351096	0.0
16	0.0	0.0	0.0	13.05732712135556	0.0
17	0.0	0.0	0.0	13.788805168182185	0.0
18	0.0	0.0	0.0	14.602014739363803	0.0
19	0.0	0.0	0.0	15.083380954358768	0.0
20	0.0010980286566383232	0.0	0.0	15.612821728363942	0.0
21	0.0011935094093894817	0.0	0.0	16.426795145567567	0.0
22	0.004821778013933506	0.0	0.0	17.09430108805092	0.0
23	0.004821778013933506	0.0	0.0	17.819047741808586	0.0
24	0.00587206629419625	0.0	0.0	18.354933466624463	0.0
25	0.00587206629419625	0.0	0.0	18.84871217947708	0.0
26	0.00587206629419625	0.0	0.0	19.286252728959266	0.0
27	0.00587206629419625	0.0	0.0	19.711094338325545	0.0
28	0.00587206629419625	0.0	0.0	20.117699123916353	0.0
29	0.00587206629419625	0.0	0.0	20.56707928673968	0.0
30	0.00587206629419625	0.0	0.0	21.04897064587478	0.0
31	0.00587206629419625	0.0	0.0	21.511765854459643	0.0
32	0.00587206629419625	0.0	0.0	21.97766418750892	0.0
33	0.00587206629419625	0.0	0.0	22.41687565016425	0.0
34	0.00587206629419625	0.0	0.0	22.850024085019882	0.0
35	0.00587206629419625	0.0	0.0	23.343277653732365	0.0
36	0.00587206629419625	0.0	0.0	23.787883778918136	0.0
37	0.00587206629419625	0.0	0.0	24.259463216756107	0.0
38	0.00587206629419625	0.0	0.0	24.73190198136884	0.0
39	0.00587206629419625	0.0	0.0	25.181234403815793	0.0
40	0.00587206629419625	0.0	0.0	25.65491441821429	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	40	5.6152203E-9	46.000004	36
CGGAACC	40	5.6152203E-9	46.000004	37
AACCGAT	40	5.6152203E-9	46.000004	23
TCGCAAA	20	6.3126476E-4	46.000004	20
GCGAATC	35	1.02010745E-7	46.000004	18
CCCTATA	20	6.3126476E-4	46.000004	42
CTATCGT	20	6.3126476E-4	46.000004	22
GCAACGC	40	5.6152203E-9	46.000004	25
TAACGGA	40	5.6152203E-9	46.000004	29
CGACACG	40	5.6152203E-9	46.000004	1
TCGATAG	40	5.6152203E-9	46.000004	35
CCGTCGA	40	5.6152203E-9	46.000004	43
CCCGTTA	20	6.3126476E-4	46.000004	37
AATGCGT	40	5.6152203E-9	46.000004	41
TACGCTT	35	1.02010745E-7	46.000004	27
CTAACGG	35	1.02010745E-7	46.000004	2
CTAACGA	40	5.6152203E-9	46.000004	21
CGGATTA	35	1.02010745E-7	46.000004	26
CTCGGTT	40	5.6152203E-9	46.000004	21
TCTAACG	20	6.3126476E-4	46.000004	39
>>END_MODULE