FastQCFastQC Report
Thu 26 May 2016
SRR1545674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545674_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1403948
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC59330.42259399920794793No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42310.30136443799912815No Hit
ATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG26600.189465706707086No Hit
ATGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT23390.1666016120255166No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT22300.1588377917130834No Hit
ATGCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC19560.13932139936806776No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC18050.12856601526552264No Hit
ATATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC17080.12165692746454998No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC16700.11895027451159161No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC16240.11567379988432619No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15920.1133945131870981No Hit
ATGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC14490.10320895075886002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGT551.8189894E-1245.00000417
ACACGGC253.891248E-545.00000444
CGTATTG502.1827873E-1145.0000043
GTCGATG453.8562575E-1045.0000043
GTCGATC253.891248E-545.00000443
ATCGTAC253.891248E-545.0000041
TAGTTCG253.891248E-545.0000042
TCGCTCA253.891248E-545.00000432
CACGTTG502.1827873E-1145.0000043
ATACGAC253.891248E-545.00000423
CAATACG253.891248E-545.00000441
ATCGCAC453.8562575E-1045.0000041
TGATCGA207.033821E-445.016
GATCGAA207.033821E-445.012
CGAGTAC207.033821E-445.016
CCTACGC207.033821E-445.015
CGTCTAG207.033821E-445.03
CGACATA207.033821E-445.035
CGATTGA351.2121927E-745.013
TAACGCG207.033821E-445.023