##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545674_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1403948
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.874411302982733	31.0	31.0	34.0	31.0	34.0
2	32.54417542530065	34.0	31.0	34.0	31.0	34.0
3	32.51650987073595	34.0	31.0	34.0	31.0	34.0
4	35.99346556994988	37.0	35.0	37.0	35.0	37.0
5	35.769827657434604	37.0	35.0	37.0	35.0	37.0
6	35.76426691017046	37.0	35.0	37.0	35.0	37.0
7	35.764130152968626	37.0	35.0	37.0	35.0	37.0
8	35.724735531515414	37.0	35.0	37.0	33.0	37.0
9	37.888241587295255	39.0	38.0	39.0	35.0	39.0
10	37.82108382931562	39.0	38.0	39.0	35.0	39.0
11	37.643719710416626	39.0	37.0	39.0	35.0	39.0
12	37.19129910794417	39.0	37.0	39.0	33.0	39.0
13	36.95972785316835	39.0	37.0	39.0	33.0	39.0
14	37.64797414149242	40.0	35.0	41.0	33.0	41.0
15	37.555381680802995	40.0	35.0	41.0	33.0	41.0
16	37.5812850618399	40.0	35.0	41.0	33.0	41.0
17	37.647503326334025	40.0	35.0	41.0	33.0	41.0
18	37.708081780806694	40.0	35.0	41.0	33.0	41.0
19	37.66001376119343	40.0	35.0	41.0	33.0	41.0
20	37.610429303649425	40.0	35.0	41.0	33.0	41.0
21	37.59348351933262	40.0	35.0	41.0	33.0	41.0
22	37.52527016670133	39.0	35.0	41.0	33.0	41.0
23	37.44398510486143	39.0	35.0	41.0	32.0	41.0
24	37.39827757153399	39.0	35.0	41.0	32.0	41.0
25	37.31883730736466	39.0	35.0	41.0	32.0	41.0
26	37.20947855618584	39.0	35.0	41.0	32.0	41.0
27	37.11672939453598	39.0	35.0	41.0	32.0	41.0
28	37.04436774011573	39.0	35.0	41.0	31.0	41.0
29	36.94529427015815	39.0	35.0	41.0	31.0	41.0
30	36.86274705330967	39.0	35.0	41.0	31.0	41.0
31	36.80857268217911	39.0	35.0	41.0	31.0	41.0
32	36.69710701535954	39.0	35.0	41.0	31.0	41.0
33	36.6064775903381	39.0	35.0	41.0	31.0	41.0
34	36.527274514440705	39.0	35.0	41.0	30.0	41.0
35	36.42959639530809	39.0	35.0	41.0	30.0	41.0
36	36.303170060429586	38.0	35.0	41.0	30.0	41.0
37	36.19794322866659	38.0	35.0	41.0	30.0	41.0
38	36.11612182217575	38.0	35.0	41.0	30.0	41.0
39	36.05302404362555	38.0	35.0	40.0	30.0	41.0
40	35.97863239949058	38.0	35.0	40.0	30.0	41.0
41	35.94178773002989	38.0	35.0	40.0	29.0	41.0
42	35.805477125933436	38.0	35.0	40.0	29.0	41.0
43	35.690680851427544	38.0	35.0	40.0	29.0	41.0
44	35.61842959995669	37.0	34.0	40.0	29.0	41.0
45	35.54576309094069	37.0	34.0	40.0	29.0	41.0
46	35.45652403080456	37.0	34.0	40.0	28.0	41.0
47	35.37199882046914	37.0	34.0	40.0	28.0	41.0
48	35.273376221911356	37.0	34.0	40.0	28.0	41.0
49	35.23466752329858	36.0	34.0	40.0	28.0	41.0
50	35.095609666454884	36.0	34.0	40.0	28.0	41.0
51	34.91999703692729	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	8.0
10	17.0
11	15.0
12	9.0
13	22.0
14	32.0
15	40.0
16	85.0
17	200.0
18	421.0
19	719.0
20	1322.0
21	2189.0
22	3435.0
23	5030.0
24	7464.0
25	10301.0
26	13752.0
27	17004.0
28	19729.0
29	23567.0
30	28446.0
31	34707.0
32	43250.0
33	55974.0
34	90152.0
35	171697.0
36	103999.0
37	142918.0
38	213394.0
39	413834.0
40	211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.0514292552145803	91.2517415174921	3.0305253470926274	3.6663038802006915
2	3.427904737212489	2.821472020331237	90.80656833443975	2.9440549080165366
3	28.749996438614534	16.422901702912075	41.68046109969885	13.146640758774542
4	27.149082444648947	15.443307017069008	44.206124443355456	13.201486094926592
5	24.550980520646064	14.06063472436301	46.61647012567417	14.771914629316754
6	23.221444099069195	15.134178758757447	46.86028257456829	14.784094567605068
7	22.99992592318234	17.65628071694963	44.35427807867528	14.98951528119275
8	25.644397085931956	19.904654588346578	42.66432944809922	11.78661887762225
9	81.6092903725779	4.6181197594212895	9.078114004222378	4.694475863778431
10	82.48866767145222	4.571109471290959	9.144142090732705	3.796080766524116
11	75.69354420534094	7.037725043947497	10.513281118673913	6.755449632037654
12	43.71422588300991	22.629541834882776	16.102946832788678	17.553285449318636
13	34.66353454686356	23.401935114405948	23.866553462094036	18.067976876636457
14	29.692980081883373	22.86516309720873	27.292321368027878	20.14953545288002
15	22.41543134076191	26.848002917486973	31.128717018009215	19.607848723741906
16	17.548798103633466	32.31294891263779	30.28680549422058	19.85144748950816
17	18.237000230777777	24.2418522623345	38.10796411263095	19.413183394256766
18	21.499941593278383	21.17948812918997	36.7636123275221	20.556957950009544
19	21.16146751874001	21.481636071991268	29.241894998960078	28.115001410308643
20	23.186684976936466	22.32760757520934	31.694265029758938	22.791442418095258
21	26.294136250060546	25.23419670814019	27.525022294273004	20.94664474752626
22	27.845262075233556	22.103738884915966	26.84508258140615	23.20591645844433
23	25.578155316293767	24.749278463304908	28.009513172852557	21.663053047548768
24	28.014570340212032	21.601298623595746	25.50151430109947	24.882616735092753
25	27.494821745534736	25.085402023436764	23.47159581408998	23.94818041693852
26	27.67246365249995	21.950599309945954	28.469359264018323	21.90757777353577
27	27.407354118528605	22.675554935083067	27.0886813471724	22.828409599215927
28	23.668967796528076	26.51045480316935	25.507853567226135	24.31272383307644
29	23.533421465752294	27.06425024288649	25.769544171151637	23.63278412020958
30	23.276431890639824	26.71081834939756	28.93725408633368	21.07549567362894
31	23.210902398094515	25.33477023365538	28.117209469296583	23.337117898953522
32	24.9240000341893	27.13298498234977	26.553333884160953	21.389681099299974
33	27.004775105630692	26.80540874733252	24.0267445802836	22.163071566753185
34	28.3892280910689	25.94205768304809	25.43406166040338	20.23465256547963
35	27.160621333553664	26.53716519415249	23.776949003809257	22.525264468484586
36	22.63801793228809	25.705795371338542	27.873468248111756	23.782718448261615
37	23.547667007609967	25.263115158111273	29.557789889654035	21.63142794462473
38	26.55283529019593	28.663882138084883	23.113035525532286	21.670247046186898
39	25.173154561280047	28.95121471735421	26.587451956910087	19.28817876445566
40	23.512266836093644	28.542652576876065	26.565513822449265	21.379566764581025
41	22.715086313738116	28.257314373466823	25.692831928248054	23.334767384547007
42	25.685922840447084	24.339362996350292	27.29531293181799	22.679401231384638
43	21.241598691689436	25.470102881303298	27.484921093943647	25.803377333063615
44	22.488439742782496	25.489120679683293	26.071051064569346	25.95138851296487
45	22.72014348109759	23.530216218834315	26.428685392906292	27.320954907161806
46	24.291284292580638	25.42387609797514	26.61622795146259	23.668611657981632
47	22.335727534068216	26.82093638795739	25.83058631801178	25.01274975996262
48	21.752799961252126	27.891061492305987	25.573169376643577	24.78296916979831
49	23.12557160236704	24.502260767492814	28.855484676070624	23.516682954069523
50	23.137680312946063	24.02930877781798	30.08615703715522	22.746853872080735
51	22.46799739021673	24.391074313293654	27.88450854305145	25.256419753438163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	466.0
1	1077.0
2	1688.0
3	4333.5
4	6979.0
5	4812.5
6	2646.0
7	2681.5
8	2717.0
9	2778.0
10	2839.0
11	3031.0
12	3223.0
13	3304.5
14	3386.0
15	3288.5
16	3191.0
17	2847.5
18	2504.0
19	2856.5
20	3209.0
21	4528.5
22	5848.0
23	6062.0
24	6276.0
25	8498.5
26	14303.0
27	17885.0
28	19902.0
29	21919.0
30	23274.0
31	24629.0
32	27974.0
33	31319.0
34	34471.5
35	37624.0
36	41217.0
37	44810.0
38	53981.0
39	63152.0
40	76146.0
41	89140.0
42	103071.0
43	117002.0
44	124202.0
45	131402.0
46	127243.0
47	123084.0
48	110598.0
49	98112.0
50	91481.0
51	84850.0
52	82571.5
53	80293.0
54	77761.5
55	75230.0
56	74366.0
57	73502.0
58	71321.5
59	69141.0
60	63376.0
61	57611.0
62	48211.5
63	38812.0
64	31909.0
65	25006.0
66	20954.5
67	16903.0
68	13538.0
69	10173.0
70	8788.5
71	7404.0
72	6134.5
73	4865.0
74	3671.0
75	1843.0
76	1209.0
77	791.5
78	374.0
79	242.0
80	110.0
81	88.0
82	66.0
83	47.5
84	29.0
85	67.0
86	105.0
87	56.0
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1403948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.887560355743485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63946562755721	12.267033599943268
2	11.202874740331007	3.7837844667034806
3	4.570660363368469	2.3156190825596843
4	2.3397467557706664	1.580504582209286
5	1.3472753926940415	1.1376097254964317
6	0.8638019736266433	0.8752504779018171
7	0.5603629653457866	0.6624214378880257
8	0.4016333255044177	0.5426085620267059
9	0.31487555658933947	0.47857319698057144
>10	2.767284266584366	10.97516298721157
>50	1.3524637407713571	16.821014600057474
>100	1.6260052481223475	45.39459322653462
>500	0.00635158300047792	0.6771854399167044
>1k	0.006775021867176449	2.0643787185802895
>5k	4.2343886669852804E-4	0.4242598959900536
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	5933	0.42259399920794793	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4231	0.30136443799912815	No Hit
ATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	2660	0.189465706707086	No Hit
ATGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	2339	0.1666016120255166	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	2230	0.1588377917130834	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	1956	0.13932139936806776	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	1805	0.12856601526552264	No Hit
ATATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	1708	0.12165692746454998	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	1670	0.11895027451159161	No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1624	0.11567379988432619	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1592	0.1133945131870981	No Hit
ATGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	1449	0.10320895075886002	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.122770928837821E-5	0.0	0.0	0.16909458185060985	0.0
2	7.122770928837821E-5	0.0	0.0	0.7660540133965076	0.0
3	7.122770928837821E-5	0.0	0.0	1.039212278517438	0.0
4	7.122770928837821E-5	0.0	0.0	1.3907210238555845	0.0
5	7.122770928837821E-5	0.0	0.0	2.681509571579574	0.0
6	7.122770928837821E-5	0.0	0.0	2.9708365267089665	0.0
7	7.122770928837821E-5	0.0	0.0	3.403331177507999	0.0
8	7.122770928837821E-5	0.0	0.0	4.757512386498645	0.0
9	7.122770928837821E-5	0.0	0.0	4.929527304430079	0.0
10	7.122770928837821E-5	0.0	0.0	5.26985329940995	0.0
11	7.122770928837821E-5	0.0	0.0	5.527056557650283	0.0
12	7.122770928837821E-5	0.0	0.0	6.187479878172126	0.0
13	7.122770928837821E-5	0.0	0.0	6.809440235678244	0.0
14	1.4245541857675642E-4	0.0	0.0	7.061942465105545	0.0
15	2.136831278651346E-4	0.0	0.0	7.203187012624399	0.0
16	2.136831278651346E-4	0.0	0.0	7.31031348739412	0.0
17	2.136831278651346E-4	0.0	0.0	7.451344351785109	0.0
18	2.136831278651346E-4	0.0	0.0	7.767809064153373	0.0
19	2.136831278651346E-4	0.0	0.0	8.155287802682151	0.0
20	2.136831278651346E-4	0.0	0.0	8.596044867758636	0.0
21	3.56138546441891E-4	0.0	0.0	8.888149703550274	7.122770928837821E-5
22	3.56138546441891E-4	0.0	0.0	9.172704402157345	7.122770928837821E-5
23	3.56138546441891E-4	0.0	0.0	9.607692022781471	7.122770928837821E-5
24	3.56138546441891E-4	0.0	0.0	9.973873676233023	7.122770928837821E-5
25	3.56138546441891E-4	0.0	0.0	10.374387085561574	7.122770928837821E-5
26	3.56138546441891E-4	0.0	0.0	10.700966132648787	7.122770928837821E-5
27	3.56138546441891E-4	0.0	0.0	11.000906016462148	7.122770928837821E-5
28	4.273662557302692E-4	0.0	0.0	11.2727109551066	7.122770928837821E-5
29	4.273662557302692E-4	0.0	0.0	11.533831737357794	7.122770928837821E-5
30	4.273662557302692E-4	0.0	0.0	11.797160578596928	7.122770928837821E-5
31	4.273662557302692E-4	0.0	0.0	12.073452862926548	7.122770928837821E-5
32	4.273662557302692E-4	0.0	0.0	12.350599879767627	7.122770928837821E-5
33	4.273662557302692E-4	0.0	0.0	12.652035545476043	7.122770928837821E-5
34	5.698216743070257E-4	0.0	0.0	12.945636163162739	7.122770928837821E-5
35	5.698216743070257E-4	0.0	0.0	13.244151492790332	7.122770928837821E-5
36	7.12277092883782E-4	0.0	0.0	13.538535615279198	7.122770928837821E-5
37	7.12277092883782E-4	0.0	0.0	13.863761335889933	7.122770928837821E-5
38	7.12277092883782E-4	0.0	0.0	14.18008359283962	7.122770928837821E-5
39	7.12277092883782E-4	0.0	0.0	14.489354306569759	7.122770928837821E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGT	55	1.8189894E-12	45.000004	17
ACACGGC	25	3.891248E-5	45.000004	44
CGTATTG	50	2.1827873E-11	45.000004	3
GTCGATG	45	3.8562575E-10	45.000004	3
GTCGATC	25	3.891248E-5	45.000004	43
ATCGTAC	25	3.891248E-5	45.000004	1
TAGTTCG	25	3.891248E-5	45.000004	2
TCGCTCA	25	3.891248E-5	45.000004	32
CACGTTG	50	2.1827873E-11	45.000004	3
ATACGAC	25	3.891248E-5	45.000004	23
CAATACG	25	3.891248E-5	45.000004	41
ATCGCAC	45	3.8562575E-10	45.000004	1
TGATCGA	20	7.033821E-4	45.0	16
GATCGAA	20	7.033821E-4	45.0	12
CGAGTAC	20	7.033821E-4	45.0	16
CCTACGC	20	7.033821E-4	45.0	15
CGTCTAG	20	7.033821E-4	45.0	3
CGACATA	20	7.033821E-4	45.0	35
CGATTGA	35	1.2121927E-7	45.0	13
TAACGCG	20	7.033821E-4	45.0	23
>>END_MODULE