FastQCFastQC Report
Thu 26 May 2016
SRR1545673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545673_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1154679
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT46640.4039217825906594Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT35990.3116883566774835No Hit
TGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC33350.2888248595497103No Hit
GTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC32510.28155011046360073No Hit
TTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC29550.2559152803506429No Hit
GCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC27960.2421452195805068No Hit
ATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC26620.23054026270504618No Hit
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC23380.20248051623005184No Hit
TCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC18080.15658031366293143No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC17390.15060462691362708No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT14910.12912679627844623No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC12940.11206577758840336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA351.01957994E-746.00000431
CGTTAAT351.01957994E-746.00000422
GTCAAGC700.046.00000416
TATCGGA351.01957994E-746.00000423
CGCTTTA351.01957994E-746.00000418
CGGACAA206.311467E-446.011
AACCGTA206.311467E-446.015
CTATGCG206.311467E-446.01
AGCCGAC301.86141E-646.013
GTCGATC253.417165E-546.041
GTCGACG301.86141E-646.01
CGAGTGT206.311467E-446.035
ACGTTGT206.311467E-446.037
ACGCATA206.311467E-446.020
AACCCGT206.311467E-446.023
GTTAGTC206.311467E-446.016
AAGCGTA253.417165E-546.024
CGATTGA206.311467E-446.011
TACGGGT301.86141E-646.04
TCGATGA301.86141E-646.01
Produced by FastQC (version 0.11.5)