##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545673_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1154679
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54573089144256	33.0	31.0	34.0	31.0	34.0
2	32.95504984502186	34.0	31.0	34.0	31.0	34.0
3	33.04035407243052	34.0	33.0	34.0	31.0	34.0
4	36.40782243376731	37.0	37.0	37.0	35.0	37.0
5	36.38863528305269	37.0	37.0	37.0	35.0	37.0
6	36.39685315139532	37.0	37.0	37.0	35.0	37.0
7	36.47548452860059	37.0	37.0	37.0	35.0	37.0
8	36.50886869857337	37.0	37.0	37.0	35.0	37.0
9	38.31239071638092	39.0	39.0	39.0	37.0	39.0
10	38.079315550035986	39.0	39.0	39.0	35.0	39.0
11	37.92125950155844	39.0	38.0	39.0	35.0	39.0
12	37.51453780661119	39.0	37.0	39.0	35.0	39.0
13	37.450294843848376	39.0	37.0	39.0	35.0	39.0
14	38.77141699121574	41.0	38.0	41.0	35.0	41.0
15	38.79402240795927	41.0	38.0	41.0	35.0	41.0
16	38.853246659894225	41.0	38.0	41.0	35.0	41.0
17	38.78792547539186	41.0	38.0	41.0	35.0	41.0
18	38.7786804817616	40.0	38.0	41.0	35.0	41.0
19	38.754950943075954	40.0	38.0	41.0	35.0	41.0
20	38.67723756992203	40.0	37.0	41.0	35.0	41.0
21	38.60469273278547	40.0	37.0	41.0	35.0	41.0
22	38.7010796940102	40.0	37.0	41.0	35.0	41.0
23	38.68911186572199	40.0	37.0	41.0	35.0	41.0
24	38.658103247742446	40.0	37.0	41.0	35.0	41.0
25	38.56922140265823	40.0	37.0	41.0	35.0	41.0
26	38.47034024174684	40.0	37.0	41.0	35.0	41.0
27	38.35537755514736	40.0	36.0	41.0	35.0	41.0
28	38.29219202912671	40.0	36.0	41.0	35.0	41.0
29	38.27271562053177	40.0	36.0	41.0	35.0	41.0
30	38.19565091250469	40.0	36.0	41.0	34.0	41.0
31	38.137910189758365	40.0	36.0	41.0	34.0	41.0
32	38.08823144787426	40.0	35.0	41.0	34.0	41.0
33	38.01395279553885	40.0	35.0	41.0	34.0	41.0
34	37.894733514682436	40.0	35.0	41.0	34.0	41.0
35	37.84748661749283	40.0	35.0	41.0	34.0	41.0
36	37.77208037904907	40.0	35.0	41.0	34.0	41.0
37	37.67248213572776	40.0	35.0	41.0	33.0	41.0
38	37.578213512153596	40.0	35.0	41.0	33.0	41.0
39	37.51501672759269	40.0	35.0	41.0	33.0	41.0
40	37.37649684457758	39.0	35.0	41.0	33.0	41.0
41	37.25790371176751	39.0	35.0	41.0	33.0	41.0
42	37.18166953759443	39.0	35.0	41.0	33.0	41.0
43	37.07360747012806	39.0	35.0	41.0	33.0	41.0
44	36.93776971781768	39.0	35.0	41.0	33.0	41.0
45	36.830126814465316	39.0	35.0	41.0	32.0	41.0
46	36.794270095844816	38.0	35.0	41.0	32.0	41.0
47	36.74581593672354	38.0	35.0	41.0	33.0	41.0
48	36.574706909885776	38.0	35.0	40.0	32.0	41.0
49	36.44857834948068	38.0	35.0	40.0	32.0	41.0
50	36.32516223123483	37.0	35.0	40.0	32.0	41.0
51	36.18128068493495	37.0	35.0	40.0	32.0	41.0
52	35.8429173822335	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	8.0
13	16.0
14	20.0
15	37.0
16	47.0
17	98.0
18	203.0
19	318.0
20	516.0
21	852.0
22	1254.0
23	1859.0
24	2782.0
25	3532.0
26	4498.0
27	5697.0
28	7097.0
29	9027.0
30	11462.0
31	14948.0
32	19631.0
33	27563.0
34	63739.0
35	135682.0
36	69611.0
37	102586.0
38	174946.0
39	495966.0
40	680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.24897655538899	14.063215837475177	45.55283329825865	12.134974308877187
2	25.1257708852417	12.761468771840487	50.20139796428271	11.9113623786351
3	25.49331892240181	13.979642827140704	45.581066253045215	14.945971997412268
4	23.59469601508298	14.803941181921557	46.75697748032137	14.844385322674094
5	23.023195191044437	17.499235718325178	44.146381808277454	15.331187282352932
6	24.690585002411925	19.61315655693054	43.70132305168796	11.994935388969576
7	85.92907639265978	2.988189791275324	6.293783813510075	4.788950002554823
8	87.03553108699474	3.015123683725087	6.6417593114623195	3.307585917817853
9	79.51283430286685	4.647005791219898	8.918062942168344	6.9220969637449015
10	44.98635551525575	22.338762547859623	13.734639670419224	18.940242266465397
11	35.58850555002732	21.95268122136109	24.170007421976152	18.288805806635438
12	30.626520444210037	22.755934766285694	25.86796850033646	20.749576289167813
13	22.733590894092647	25.977089736628102	31.681705478319085	19.60761389096017
14	17.414190437342324	33.26967927882987	29.557392141019278	19.75873814280852
15	18.43811137121226	24.200145668190036	36.935459984982835	20.426282975614868
16	21.64575609325189	21.94168249357614	35.19454324535217	21.218018167819803
17	21.35952935837579	21.988188925233768	27.466767820320626	29.185513896069814
18	23.015660629490966	22.697217148662098	29.689030457815548	24.598091764031388
19	25.85792241826516	25.929803867568392	26.72855399639207	21.483719717774378
20	28.524204562480136	21.19073785874689	25.398400767659236	24.88665681111374
21	25.52969266783236	25.64504940334067	27.30603050717992	21.519227421647056
22	28.448339322010707	21.213254939251517	24.57193730898371	25.76646842975407
23	27.911133743663825	25.48691021487357	21.404736727696616	25.197219313765988
24	27.59017874231713	21.09833122452214	27.28515890563525	24.026331127525484
25	28.698971748858344	22.469967843876955	24.678027399822806	24.153033007441895
26	23.504714297220268	26.364903146242373	24.147836758094673	25.982545798442686
27	25.315693798882634	25.165089171969008	23.81588302896303	25.703334000185336
28	25.314741153168974	25.38489051935646	27.389776725825964	21.910591601648598
29	25.052676977757454	24.10063749319075	25.87221210397002	24.974473425081776
30	26.379972269349317	26.966282404027442	24.797887551432044	21.8558577751912
31	27.95911244596983	27.263767679155855	21.680830776345633	23.096289098528683
32	29.749047137775953	25.665834400729555	24.13865671758125	20.446461743913243
33	27.338940086378983	27.873287727584895	22.691154857757002	22.096617328279113
34	22.526693565917455	25.958643051445467	27.68925389653748	23.8254094860996
35	24.27029503437752	24.385824978197405	29.322521670524882	22.021358316900194
36	27.293299696279227	27.98587313010802	22.2835090964675	22.43731807714525
37	26.379625852726168	28.053684184089256	26.123450759908163	19.44323920327641
38	22.97495667627107	28.346839251428317	27.07436439044964	21.60383968185097
39	22.892163103338678	28.238670660850328	25.562862059498787	23.306304176312203
40	26.77653269869808	23.722350540713048	27.41835609723568	22.08276066335319
41	22.07054947738722	24.502307567730945	27.395665808419484	26.03147714646235
42	23.35644798251289	24.871068063072073	24.829151651671157	26.943332302743876
43	23.605867951179505	22.312175072033007	26.584444681162474	27.497512295625015
44	27.22470920489591	24.024166023630812	24.44930582438929	24.301818947083994
45	22.94464522174561	25.28330384461829	25.06073116424565	26.71131976939045
46	22.285154575427455	26.800522049851082	25.061857018270878	25.85246635645058
47	24.440472200499013	23.317649320720303	28.96406706972241	23.277811409058273
48	23.14340175927682	22.69557166970214	31.376858849948775	22.78416772107226
49	22.917711329295848	22.889045353730346	27.15378040130634	27.03946291566747
50	21.438512348453553	24.44835317867563	30.30876979662746	23.804364676243353
51	22.862371273747943	25.316906257063653	26.711233165234667	25.10948930395374
52	22.17109690225595	22.97348440562269	28.923276512346725	25.932142179774637
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	173.0
1	140.0
2	107.0
3	101.0
4	95.0
5	122.5
6	150.0
7	209.0
8	268.0
9	361.0
10	454.0
11	588.5
12	723.0
13	830.0
14	858.5
15	780.0
16	839.5
17	899.0
18	971.5
19	1044.0
20	1531.5
21	2019.0
22	2195.0
23	2371.0
24	2852.5
25	3334.0
26	5368.0
27	7402.0
28	8390.0
29	9378.0
30	12324.5
31	15271.0
32	16316.5
33	17362.0
34	19385.5
35	21409.0
36	22783.5
37	24158.0
38	28639.5
39	41522.5
40	49924.0
41	58306.0
42	66688.0
43	75283.5
44	83879.0
45	97710.0
46	111541.0
47	114329.0
48	117117.0
49	104011.5
50	90906.0
51	82671.0
52	74436.0
53	69195.0
54	63954.0
55	64277.5
56	64601.0
57	61592.5
58	58584.0
59	59076.0
60	59568.0
61	54445.0
62	49322.0
63	44674.0
64	34350.5
65	28675.0
66	23796.5
67	18918.0
68	16078.5
69	13239.0
70	10207.0
71	7175.0
72	6778.5
73	6382.0
74	5407.0
75	4432.0
76	3205.0
77	1978.0
78	1492.0
79	1006.0
80	760.0
81	514.0
82	324.0
83	134.0
84	124.0
85	114.0
86	99.5
87	85.0
88	51.5
89	11.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1154679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.677531461033684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.56053698661367	12.296585810272603
2	12.17702701726527	4.305195563991279
3	5.019325130472254	2.6618783372124075
4	2.6710983953442677	1.8887370367685947
5	1.5673644448192057	1.3853567142098546
6	1.029083289294149	1.0914991333512807
7	0.7009326348169788	0.8673531092829663
8	0.5018444089152387	0.7097096261714387
9	0.374850124577114	0.5963782383345894
>10	3.3756288341697096	14.030665814381832
>50	1.5324796596292263	19.535634255072708
>100	1.4790438685450054	37.21454090370491
>500	0.003921892922890303	0.45982714953567516
>1k	0.006863312615058029	2.956638307709881
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT	4664	0.4039217825906594	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	3599	0.3116883566774835	No Hit
TGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	3335	0.2888248595497103	No Hit
GTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	3251	0.28155011046360073	No Hit
TTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	2955	0.2559152803506429	No Hit
GCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	2796	0.2421452195805068	No Hit
ATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	2662	0.23054026270504618	No Hit
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	2338	0.20248051623005184	No Hit
TCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1808	0.15658031366293143	No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1739	0.15060462691362708	No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	1491	0.12912679627844623	No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1294	0.11206577758840336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42219525946172054	0.0
2	0.0	0.0	0.0	0.9924836253192446	0.0
3	0.0	0.0	0.0	3.3517540372692323	0.0
4	0.0	0.0	0.0	3.849901141356169	0.0
5	0.0	0.0	0.0	4.596428964240278	0.0
6	0.0	0.0	0.0	7.02558892991039	0.0
7	0.0	0.0	0.0	7.331734620617505	0.0
8	0.0	0.0	0.0	7.937097669568772	0.0
9	0.0	0.0	0.0	8.310101768543465	0.0
10	0.0	0.0	0.0	9.494673411398319	0.0
11	0.0	0.0	0.0	10.542237279798108	0.0
12	0.0	0.0	0.0	10.98937453612649	0.0
13	0.0	0.0	0.0	11.211340987408622	0.0
14	0.0	0.0	0.0	11.351986136406742	0.0
15	0.0	0.0	0.0	11.554986277571516	0.0
16	0.0	0.0	0.0	12.047850528155443	0.0
17	0.0	0.0	0.0	12.707341174473598	0.0
18	0.0	0.0	0.0	13.428927000491045	0.0
19	0.0	0.0	0.0	13.890960171614795	0.0
20	0.0	0.0	0.0	14.339223281968408	0.0
21	0.0	0.0	0.0	15.037079569300213	0.0
22	0.0	0.0	0.0	15.619752329435281	0.0
23	0.0	0.0	0.0	16.240011293181915	0.0
24	0.0	0.0	0.0	16.73729235571098	0.0
25	0.0	0.0	0.0	17.17992619593844	0.0
26	0.0	0.0	0.0	17.576486625287203	0.0
27	0.0	0.0	0.0	17.954686973609114	0.0
28	0.0	0.0	0.0	18.335052425825705	0.0
29	0.0	0.0	0.0	18.71446523232864	0.0
30	0.0	0.0	0.0	19.116048702713048	0.0
31	8.66041557870196E-5	0.0	0.0	19.52750504685718	0.0
32	8.66041557870196E-5	0.0	0.0	19.92241999724599	0.0
33	8.66041557870196E-5	0.0	0.0	20.312831531533874	0.0
34	8.66041557870196E-5	0.0	0.0	20.699778899590275	0.0
35	8.66041557870196E-5	0.0	0.0	21.13522459488741	0.0
36	8.66041557870196E-5	0.0	0.0	21.538107127608626	0.0
37	8.66041557870196E-5	0.0	0.0	21.931289994881695	0.0
38	8.66041557870196E-5	0.0	0.0	22.34846221330777	0.0
39	8.66041557870196E-5	0.0	0.0	22.77758580523245	0.0
40	8.66041557870196E-5	0.0	0.0	23.222298145198796	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	35	1.01957994E-7	46.000004	31
CGTTAAT	35	1.01957994E-7	46.000004	22
GTCAAGC	70	0.0	46.000004	16
TATCGGA	35	1.01957994E-7	46.000004	23
CGCTTTA	35	1.01957994E-7	46.000004	18
CGGACAA	20	6.311467E-4	46.0	11
AACCGTA	20	6.311467E-4	46.0	15
CTATGCG	20	6.311467E-4	46.0	1
AGCCGAC	30	1.86141E-6	46.0	13
GTCGATC	25	3.417165E-5	46.0	41
GTCGACG	30	1.86141E-6	46.0	1
CGAGTGT	20	6.311467E-4	46.0	35
ACGTTGT	20	6.311467E-4	46.0	37
ACGCATA	20	6.311467E-4	46.0	20
AACCCGT	20	6.311467E-4	46.0	23
GTTAGTC	20	6.311467E-4	46.0	16
AAGCGTA	25	3.417165E-5	46.0	24
CGATTGA	20	6.311467E-4	46.0	11
TACGGGT	30	1.86141E-6	46.0	4
TCGATGA	30	1.86141E-6	46.0	1
>>END_MODULE