##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545672_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1610624
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.867932552849084	31.0	31.0	34.0	31.0	34.0
2	32.540627110983074	34.0	31.0	34.0	31.0	34.0
3	32.48526720078677	34.0	31.0	34.0	31.0	34.0
4	35.96197498609234	37.0	35.0	37.0	35.0	37.0
5	35.72263358797584	37.0	35.0	37.0	35.0	37.0
6	35.71787704641183	37.0	35.0	37.0	33.0	37.0
7	35.70482930839228	37.0	35.0	37.0	33.0	37.0
8	35.657360749622505	37.0	35.0	37.0	33.0	37.0
9	37.85633953051737	39.0	38.0	39.0	35.0	39.0
10	37.82520625546372	39.0	38.0	39.0	35.0	39.0
11	37.63038486946674	39.0	37.0	39.0	35.0	39.0
12	37.1325449018517	39.0	37.0	39.0	33.0	39.0
13	36.879754058054516	39.0	37.0	39.0	33.0	39.0
14	37.642300127155686	40.0	36.0	41.0	33.0	41.0
15	37.57705895354844	40.0	36.0	41.0	32.0	41.0
16	37.592910573790036	40.0	36.0	41.0	32.0	41.0
17	37.68223371811174	40.0	36.0	41.0	33.0	41.0
18	37.740345356830645	40.0	36.0	41.0	33.0	41.0
19	37.6931797862195	40.0	36.0	41.0	33.0	41.0
20	37.63393194190574	40.0	36.0	41.0	32.0	41.0
21	37.62426239767941	39.0	36.0	41.0	33.0	41.0
22	37.52100428157037	39.0	35.0	41.0	32.0	41.0
23	37.483113377175556	39.0	35.0	41.0	32.0	41.0
24	37.461793069021695	39.0	35.0	41.0	32.0	41.0
25	37.40176229833903	39.0	35.0	41.0	32.0	41.0
26	37.309401201025196	39.0	35.0	41.0	32.0	41.0
27	37.224499324485414	39.0	35.0	41.0	32.0	41.0
28	37.1473410305571	39.0	35.0	41.0	31.0	41.0
29	37.08802116446793	39.0	35.0	41.0	31.0	41.0
30	37.02314568763411	39.0	35.0	41.0	31.0	41.0
31	36.96409838671223	39.0	35.0	41.0	31.0	41.0
32	36.8381254718668	39.0	35.0	41.0	31.0	41.0
33	36.701607575697366	39.0	35.0	41.0	31.0	41.0
34	36.64292907593579	39.0	35.0	41.0	30.0	41.0
35	36.52981949852976	39.0	35.0	41.0	30.0	41.0
36	36.384112617221646	39.0	35.0	41.0	30.0	41.0
37	36.269537148335054	38.0	35.0	41.0	30.0	41.0
38	36.169437435428755	38.0	35.0	41.0	30.0	41.0
39	36.09343707780339	38.0	35.0	40.0	30.0	41.0
40	36.04841353413335	38.0	35.0	40.0	30.0	41.0
41	36.004048120480014	38.0	35.0	40.0	30.0	41.0
42	35.891029812048004	38.0	35.0	40.0	29.0	41.0
43	35.77037657454502	38.0	35.0	40.0	29.0	41.0
44	35.66895749721847	38.0	35.0	40.0	29.0	41.0
45	35.66263137765239	38.0	35.0	40.0	29.0	41.0
46	35.58804910394977	38.0	34.0	40.0	29.0	41.0
47	35.523310220138285	37.0	34.0	40.0	28.0	41.0
48	35.4600868979973	37.0	34.0	40.0	28.0	41.0
49	35.41121453548438	37.0	34.0	40.0	28.0	41.0
50	35.304626654017326	37.0	34.0	40.0	28.0	41.0
51	35.13224936422157	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	6.0
11	16.0
12	15.0
13	15.0
14	13.0
15	41.0
16	76.0
17	193.0
18	392.0
19	786.0
20	1360.0
21	2422.0
22	3856.0
23	5638.0
24	8111.0
25	11879.0
26	15768.0
27	19105.0
28	22858.0
29	27107.0
30	32859.0
31	41157.0
32	50707.0
33	64178.0
34	100092.0
35	177205.0
36	121320.0
37	166590.0
38	244677.0
39	491961.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.439923905269014	90.50622615830883	3.161321326392752	3.8925286100294048
2	3.3518685925454976	2.487731463085115	90.76271060160535	3.3976893427640467
3	28.098550623857587	16.182237443375982	42.305590876579515	13.41362105618692
4	26.68934524755623	15.354483728045775	44.07887874513232	13.877292279265676
5	23.848955435905587	14.380016689183819	46.932244893904475	14.83878298100612
6	21.505019172693316	14.517106413414924	48.32549372168799	15.652380692203765
7	21.913308133990306	17.36519510450608	45.13468072001907	15.586816041484541
8	24.849685587697685	19.29438528172932	43.4755722005881	12.3803569299849
9	80.61918858777716	4.5179383890964	10.124709429388858	4.738163593737582
10	82.56594959469125	4.390658527378209	9.042706429309385	4.000685448621156
11	75.40828896129699	7.486228939839466	10.352819776682825	6.75266232218072
12	40.001577028530555	24.80622417150123	16.569416573948978	18.622782226019233
13	31.646492291186522	24.181497357545894	25.893194190574587	18.278816160692998
14	26.770369744893905	24.78691488516252	29.4025172852261	19.040198084717478
15	20.66329571644282	29.001057975045697	32.323062365890486	18.012583942620996
16	16.786226953031868	33.21222085353254	31.53721787332115	18.46433432011444
17	15.186412222840339	25.580644520384645	38.96148325121195	20.27146000556306
18	21.07903520623063	21.752562981800843	36.564896586664545	20.60350522530398
19	20.147594969403162	22.049094015735516	29.465101724548994	28.338209290312328
20	22.34158934673766	22.66171372089327	32.97399020503855	22.022706727330526
21	25.389724727807362	24.840931216720975	29.273933581022014	20.495410474449656
22	25.535444647540334	22.580565048080743	27.902539636811568	23.98145066756735
23	22.91130642533577	24.98534729396805	30.64843191210363	21.454914368592544
24	27.162205455773663	22.85871811173806	25.935165501072877	24.043910931415404
25	24.33684087657951	26.50922872129063	24.458781192879282	24.695149209250577
26	23.438120877374235	23.12296352221251	30.700523523801955	22.738392076611298
27	24.555451800047685	24.32510629420647	28.42078598108559	22.698655924660255
28	21.402326054994834	27.53796044266073	26.182585134705555	24.87712836763888
29	18.980407593578636	26.532325359612173	26.69965181196853	27.787615234840658
30	20.268293530954463	25.845324545021057	30.908082830008745	22.978299094015735
31	20.67111877135818	23.825051656997537	28.91189998410554	26.591929587538743
32	21.712764742112373	26.00712518874672	27.01660971151554	25.263500357625368
33	22.47073184057856	28.374654792179925	26.672271119764762	22.482342247476755
34	25.462056941905747	25.002173070809825	25.54016331558452	23.995606671699914
35	25.089778868314394	25.343966065326235	24.75065564650719	24.815599419852184
36	22.02574902646428	25.08121076054995	28.537759278391476	24.355280934594294
37	23.314380016689185	24.698129420646904	29.241337518874673	22.74615304378924
38	25.27647669474688	26.0902606691568	25.286286557259796	23.346976078836526
39	23.277375725184772	26.02215042120321	28.690557200190735	22.009916653421282
40	22.526859155209408	25.568537411587062	28.662058928713343	23.242544504490183
41	21.42579521974092	28.31474012556624	25.50564253357705	24.75382212111579
42	24.840993304458397	24.191431395533655	27.32512367877295	23.642451621235
43	21.86773573472145	23.530507430660418	29.521539477866966	25.080217356751174
44	20.68185994993245	25.665953071604548	27.333753874274812	26.318433104188188
45	20.894945064769928	23.120480012715568	29.148764702376223	26.835810220138285
46	22.47197359532703	24.889297564173884	27.178720793928317	25.46000804657077
47	20.330505443852818	26.758138460621474	26.926706667726297	25.98464942779941
48	21.364266271954225	25.316709548597316	26.489608996264803	26.82941518318366
49	21.650677004688866	25.245805352459666	29.69613019550187	23.4073874473496
50	21.888224688071205	22.68034004212032	30.84394619725026	24.587489072558213
51	21.04811551299372	23.805742370658827	28.38750695382659	26.75863516252086
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1123.0
1	1355.0
2	1587.0
3	4279.5
4	6972.0
5	4796.5
6	2621.0
7	2791.5
8	2962.0
9	3199.5
10	3437.0
11	3541.5
12	3646.0
13	4047.0
14	4448.0
15	4109.0
16	3770.0
17	3780.5
18	3791.0
19	3950.5
20	4110.0
21	5304.0
22	6498.0
23	7039.5
24	7581.0
25	9756.5
26	15187.0
27	18442.0
28	21741.0
29	25040.0
30	27264.0
31	29488.0
32	38051.5
33	46615.0
34	52486.5
35	58358.0
36	61332.0
37	64306.0
38	76484.0
39	88662.0
40	98475.5
41	108289.0
42	107530.5
43	106772.0
44	112829.5
45	118887.0
46	133812.5
47	148738.0
48	152659.5
49	156581.0
50	146515.0
51	136449.0
52	115280.5
53	94112.0
54	84810.0
55	75508.0
56	70703.0
57	65898.0
58	64316.0
59	62734.0
60	52900.5
61	43067.0
62	39237.5
63	35408.0
64	28159.0
65	20910.0
66	17712.0
67	14514.0
68	12078.0
69	9642.0
70	8288.5
71	6935.0
72	6252.5
73	5570.0
74	4327.5
75	2253.5
76	1422.0
77	905.5
78	389.0
79	241.0
80	93.0
81	102.0
82	111.0
83	95.5
84	80.0
85	56.5
86	33.0
87	19.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1610624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.40425088723498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.64421262561218	12.295096002673276
2	11.378110052245507	3.9605496394370228
3	4.999729551440677	2.6104964244478905
4	2.8552544314521557	1.9877425788753116
5	1.7203247105603683	1.4970481435051275
6	1.1804648398435023	1.2327063741717583
7	0.8130199239730314	0.9905001913203143
8	0.5566442360872953	0.7750380751837238
9	0.42139358668573396	0.6600635734455286
>10	2.9673218409543924	11.331198514857128
>50	0.9035193275103581	11.518431499670733
>100	1.546021837534584	46.792771115020315
>500	0.007170787743666686	0.8626327300216027
>1k	0.006095169582116683	2.5689906584389006
>5k	7.170787743666687E-4	0.9167344789313777
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	8694	0.5397907891599777	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	5997	0.37234016132877695	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4350	0.2700816577922594	No Hit
ATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	3813	0.23674054279583567	No Hit
ATGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	3625	0.22506804816021614	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	3071	0.19067144162759278	Illumina Single End Adapter 2 (95% over 21bp)
ATGCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	2969	0.184338492410395	No Hit
ATTGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	2934	0.1821654216005722	No Hit
ATGTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	2744	0.17036875149010572	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	2660	0.16515338154653103	No Hit
ATATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	2507	0.15565395772073432	No Hit
ATGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	2454	0.15236330763728842	No Hit
ATAGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	2043	0.12684524755622667	No Hit
ATTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1785	0.11082661130096161	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2168724668203131	0.0
2	0.0	0.0	0.0	1.0297872129063022	0.0
3	0.0	0.0	0.0	1.3907032305491536	0.0
4	0.0	0.0	0.0	1.8383558173726455	0.0
5	0.0	0.0	0.0	3.5804135540014306	0.0
6	0.0	0.0	0.0	3.9870261463879837	0.0
7	0.0	0.0	0.0	4.566304736549313	0.0
8	0.0	0.0	0.0	6.420555014702376	0.0
9	0.0	0.0	0.0	6.6257549868870695	0.0
10	0.0	0.0	0.0	7.040501072876102	0.0
11	0.0	0.0	0.0	7.352429865691806	0.0
12	0.0	0.0	0.0	8.231778490820949	0.0
13	6.208773742350791E-5	0.0	0.0	9.071266788524198	0.0
14	6.208773742350791E-5	0.0	0.0	9.406230131924024	0.0
15	6.208773742350791E-5	0.0	0.0	9.587588412938091	0.0
16	6.208773742350791E-5	0.0	0.0	9.703382043232933	0.0
17	6.208773742350791E-5	0.0	0.0	9.885237026146388	0.0
18	0.0021730708098227766	0.0	0.0	10.284088651355002	0.0
19	0.0021730708098227766	0.0	0.0	10.809288822220456	0.0
20	0.002235158547246285	0.0	0.0	11.404834399586743	0.0
21	0.002235158547246285	0.0	0.0	11.764260311531432	0.0
22	0.0022972462846697927	0.0	0.0	12.22246781371692	0.0
23	0.0023593340220933005	0.0	0.0	12.83347323770166	0.0
24	0.0024835094969403162	0.0	0.0	13.335452594770722	0.0
25	0.0024835094969403162	0.0	0.0	13.872511523484066	0.0
26	0.002545597234363824	0.0	0.0	14.251184634030041	0.0
27	0.002545597234363824	0.0	0.0	14.616198442342844	0.0
28	0.002607684971787332	0.0	0.0	14.958488138758643	0.0
29	0.002607684971787332	0.0	0.0	15.308662977827227	0.0
30	0.002607684971787332	0.0	0.0	15.697145950886116	0.0
31	0.002607684971787332	0.0	0.0	16.043347174759596	0.0
32	0.00266977270921084	0.0	0.0	16.427794444885958	0.0
33	0.00266977270921084	0.0	0.0	16.7952917626957	0.0
34	0.00266977270921084	0.0	0.0	17.167569836287054	0.0
35	0.002731860446634348	0.0	0.0	17.555307756496862	0.0
36	0.002731860446634348	0.0	0.0	17.936961078439165	0.0
37	0.002731860446634348	0.0	0.0	18.35158298895335	0.0
38	0.002731860446634348	0.0	0.0	18.753166474608598	0.0
39	0.002731860446634348	0.0	0.0	19.160337856631966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	40	6.8175723E-9	45.000004	26
GGTCGAA	30	2.1660471E-6	45.000004	32
CGACCGT	20	7.0341304E-4	45.000004	44
CGATTGT	80	0.0	45.000004	32
TAACGCG	35	1.2123382E-7	45.000004	3
ATCCGAT	20	7.0341304E-4	45.000004	20
ATTCCGA	20	7.0341304E-4	45.000004	1
ACCTAGC	20	7.0341304E-4	45.000004	13
GCCGAAC	20	7.0341304E-4	45.000004	34
CTACGAT	30	2.1660471E-6	45.000004	12
TACGACA	35	1.2123382E-7	45.000004	17
GTGTCGA	30	2.1660471E-6	45.000004	20
CGTAATG	35	1.2123382E-7	45.000004	3
CAGTCGA	20	7.0341304E-4	45.000004	9
ACGTACT	30	2.1660471E-6	45.000004	39
CCGCGAA	40	6.8175723E-9	45.000004	44
CGCAATA	35	1.2123382E-7	45.000004	45
CGATCAT	20	7.0341304E-4	45.000004	25
TCGAAGT	20	7.0341304E-4	45.000004	2
TCGAACG	35	1.2123382E-7	45.000004	2
>>END_MODULE