FastQCFastQC Report
Thu 26 May 2016
SRR1545671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545671_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1350546
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT69790.5167539646927983Illumina Single End Adapter 2 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC57980.4293078503064687No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT57490.4256796880669003Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC56020.4147952013481955No Hit
TTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC49080.3634085769755343No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC47460.35141342834675754No Hit
GGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC43520.3222400421755349No Hit
ATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC41090.30424731923236975No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC31640.23427561889783835No Hit
TCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC20520.15193854929783954No Hit
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC19450.1440158276726598No Hit
CTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC18430.1364633266841707No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT16850.12476435456474641Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC351.01974365E-746.00000424
GCGAACC351.01974365E-746.00000424
CTGCGTA351.01974365E-746.00000427
CGGTACA351.01974365E-746.00000428
ACGGTAC351.01974365E-746.00000427
TCACGAC206.311849E-446.016
AACCGTT253.417476E-546.033
AACCGTA206.311849E-446.024
ACACGTT301.8616465E-646.037
GATCGAG253.417476E-546.043
CGAGTGT206.311849E-446.024
CAACGTA206.311849E-446.015
CAACGCG206.311849E-446.044
CGAAACG206.311849E-446.046
CATGCGG453.110472E-1046.02
ACGCACT206.311849E-446.044
GACACGT301.8616465E-646.09
GCAACGA453.110472E-1046.09
GGCGTAC206.311849E-446.038
TAGAACG301.8616465E-646.018
Produced by FastQC (version 0.11.5)