##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545671_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1350546
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51465407324149	33.0	31.0	34.0	31.0	34.0
2	32.909116757222634	34.0	31.0	34.0	31.0	34.0
3	32.982385642547534	34.0	33.0	34.0	31.0	34.0
4	36.37326310988296	37.0	37.0	37.0	35.0	37.0
5	36.35477355084536	37.0	37.0	37.0	35.0	37.0
6	36.34671606890843	37.0	37.0	37.0	35.0	37.0
7	36.45533658238964	37.0	37.0	37.0	35.0	37.0
8	36.50974864980534	37.0	37.0	37.0	35.0	37.0
9	38.30869663084412	39.0	39.0	39.0	37.0	39.0
10	38.050554368381384	39.0	39.0	39.0	35.0	39.0
11	37.865591397849464	39.0	38.0	39.0	35.0	39.0
12	37.54370898880897	39.0	37.0	39.0	35.0	39.0
13	37.50355633943605	39.0	37.0	39.0	35.0	39.0
14	38.84553210331229	41.0	38.0	41.0	35.0	41.0
15	38.88376182669824	41.0	38.0	41.0	35.0	41.0
16	38.95186317237621	41.0	38.0	41.0	35.0	41.0
17	38.88727522054043	41.0	38.0	41.0	35.0	41.0
18	38.879929302667215	41.0	38.0	41.0	35.0	41.0
19	38.86580982802511	40.0	38.0	41.0	35.0	41.0
20	38.75888788682503	40.0	38.0	41.0	35.0	41.0
21	38.742741083976405	40.0	38.0	41.0	35.0	41.0
22	38.84051413280258	40.0	38.0	41.0	35.0	41.0
23	38.850987674614565	41.0	38.0	41.0	35.0	41.0
24	38.81714358489085	41.0	38.0	41.0	35.0	41.0
25	38.747882708178764	41.0	38.0	41.0	35.0	41.0
26	38.65797832876481	40.0	38.0	41.0	35.0	41.0
27	38.57825279553603	40.0	37.0	41.0	35.0	41.0
28	38.53983351918409	40.0	37.0	41.0	35.0	41.0
29	38.53029589514167	40.0	37.0	41.0	35.0	41.0
30	38.47822140082604	40.0	37.0	41.0	35.0	41.0
31	38.41807831795437	40.0	37.0	41.0	35.0	41.0
32	38.34326339125065	40.0	37.0	41.0	35.0	41.0
33	38.24180368532431	40.0	36.0	41.0	34.0	41.0
34	38.07965593174909	40.0	36.0	41.0	34.0	41.0
35	38.051733891329874	40.0	36.0	41.0	34.0	41.0
36	37.94698144306081	40.0	36.0	41.0	34.0	41.0
37	37.83986032315819	40.0	35.0	41.0	34.0	41.0
38	37.783705997426225	40.0	35.0	41.0	34.0	41.0
39	37.73548179773218	40.0	35.0	41.0	33.0	41.0
40	37.634524851430456	40.0	35.0	41.0	33.0	41.0
41	37.48369844492524	40.0	35.0	41.0	33.0	41.0
42	37.41116851999117	39.0	35.0	41.0	33.0	41.0
43	37.37475361816628	39.0	35.0	41.0	33.0	41.0
44	37.276866541384	39.0	35.0	41.0	33.0	41.0
45	37.202723194915244	39.0	35.0	41.0	33.0	41.0
46	37.18564713826852	39.0	35.0	41.0	33.0	41.0
47	37.153956992209075	39.0	35.0	41.0	33.0	41.0
48	37.04808795849975	39.0	35.0	41.0	33.0	41.0
49	36.97357661271811	39.0	35.0	41.0	33.0	41.0
50	36.88922035976561	39.0	35.0	41.0	33.0	41.0
51	36.75993116857923	38.0	35.0	41.0	32.0	41.0
52	36.45386014249052	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	7.0
13	10.0
14	14.0
15	16.0
16	46.0
17	86.0
18	140.0
19	305.0
20	565.0
21	914.0
22	1333.0
23	1997.0
24	2790.0
25	3757.0
26	4718.0
27	6109.0
28	7959.0
29	10241.0
30	13268.0
31	16683.0
32	22272.0
33	31015.0
34	67577.0
35	133948.0
36	79417.0
37	113768.0
38	195701.0
39	635143.0
40	742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.225325164785204	13.986639477663108	45.53691618056697	12.251119176984716
2	25.193958591562225	12.986229273197655	49.838805934784894	11.981006200455223
3	25.262301321095322	13.921480645605556	45.154922527629566	15.661295505669559
4	21.840203887909038	14.119770818617065	48.80559418190865	15.23443111156525
5	23.253484146411896	16.61787158675084	44.44683853789505	15.68180572894222
6	24.372290910491014	18.873996146743615	44.153401661254044	12.600311281511328
7	84.38009516151246	2.379926340902124	7.683188873240897	5.556789624344524
8	86.4802087452038	2.547784377577661	7.303860808887665	3.6681460683308824
9	78.36719371276506	4.395555575300656	9.452029031221446	7.785221680712838
10	40.421355511030356	23.969490857771596	14.801939363783239	20.807214267414807
11	30.79843263391251	22.70726061903852	27.79638753511543	18.697919211933545
12	27.034769641315435	24.50912445781188	28.828044361317573	19.628061539555112
13	20.74257374424862	27.436829252761473	34.484127160422524	17.336469842567375
14	16.37404427542638	34.2335618335103	30.99879604248948	18.393597848573837
15	14.683838980678926	25.262153232840646	38.02973019800881	22.024277588471623
16	20.39130840415654	22.931021971854346	35.22545696333187	21.452212660657246
17	19.657234925726335	23.084885668462977	28.010893372013985	29.246986033796702
18	21.42155839193926	23.170036414901826	31.448466027813936	23.959939165344977
19	24.20591375636224	26.149498054860775	28.82730392004419	20.817284268732795
20	25.336493536688128	21.527737670542134	26.73355813130393	26.40221066146581
21	22.51422757906802	26.022882597112574	30.456200677355678	21.006689146463728
22	26.70912356928235	22.344222262699677	26.058868042998906	24.887786125019066
23	24.28047619259174	27.317840340129102	22.566206556459388	25.83547691081977
24	23.56735720219822	21.898032351360115	30.172537625523304	24.362072820918353
25	26.949544850749252	24.01569439323059	25.380475748326976	23.654285007693186
26	21.15722085734214	27.819045038080898	25.618231441209705	25.40550266336726
27	19.17076500911483	24.80567118780108	24.941171940829857	31.082391862254227
28	20.745239332832796	23.36447629329175	31.284310197505306	24.60597417637015
29	19.570899473250076	22.731991357569456	27.00729927007299	30.689809899107473
30	20.482086504273088	25.313095592449276	25.376107144814025	28.828710758463615
31	21.88248308461911	29.26616346277728	25.008107831943526	23.84324562066009
32	26.837145865449973	24.241677069866558	22.46454396962414	26.456633095059335
33	25.675837772278765	24.182960076887422	24.156674411682385	25.984527739151424
34	20.746424038870206	25.431492152062944	28.029552492103193	25.792531316963657
35	23.656728463895345	22.863715860103987	29.330655897688786	24.148899778311883
36	25.951948323122647	23.710114279706136	25.431566196190282	24.906371200980935
37	24.33460244967591	24.240566407956486	28.34920098982189	23.075630152545713
38	21.237336603122	24.60560395573346	30.403629346945603	23.753430094198936
39	22.066186564545006	28.15002228728233	24.703564336201804	25.080226811970864
40	26.97731139850105	22.273362032837092	27.546858825985936	23.202467742675925
41	22.540513244273058	22.166146136451477	29.409883113940584	25.883457505334878
42	20.25913963685798	25.196179915382373	26.78420431440321	27.760476133356438
43	21.015130176980275	21.36624742881768	29.474375548852095	28.144246845349958
44	25.492060248225535	22.436629333617663	24.76213324092626	27.30917717723054
45	20.248773459030645	24.303874136830586	26.63981826609386	28.80753413804491
46	21.57549613267523	23.9489065903716	25.49050532155143	28.985091955401742
47	22.693932676117658	23.445258436217646	29.571521443919718	24.289287443744975
48	20.904656338991785	20.606406594073803	33.427739595689445	25.061197471244963
49	20.979885172367325	21.52973686198027	27.74329789581399	29.74708006983842
50	21.266658077547895	21.93031559087954	31.84519446209163	24.957831869480938
51	23.764980978063686	21.703296296460838	27.78528091601471	26.746441809460762
52	21.482274576356524	21.244148662837105	29.62638814227727	27.6471886185291
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	322.0
1	251.5
2	181.0
3	156.5
4	132.0
5	175.0
6	218.0
7	316.5
8	415.0
9	579.0
10	743.0
11	820.0
12	897.0
13	970.5
14	1114.5
15	1185.0
16	1371.0
17	1557.0
18	1527.0
19	1497.0
20	1949.5
21	2402.0
22	2598.0
23	2794.0
24	3671.0
25	4548.0
26	6355.0
27	8162.0
28	9255.0
29	10348.0
30	12875.5
31	15403.0
32	20138.0
33	24873.0
34	29855.5
35	34838.0
36	37069.0
37	39300.0
38	41971.5
39	52493.0
40	60343.0
41	66614.5
42	72886.0
43	77745.5
44	82605.0
45	86590.0
46	90575.0
47	102207.5
48	113840.0
49	132084.0
50	150328.0
51	150126.5
52	149925.0
53	128378.0
54	106831.0
55	92100.0
56	77369.0
57	67760.0
58	58151.0
59	51978.0
60	45805.0
61	43506.5
62	41208.0
63	37073.5
64	28406.0
65	23873.0
66	19074.5
67	14276.0
68	13638.5
69	13001.0
70	10457.5
71	7914.0
72	6373.0
73	4832.0
74	4270.5
75	3709.0
76	3172.0
77	2635.0
78	1811.5
79	988.0
80	742.5
81	497.0
82	344.5
83	192.0
84	178.0
85	164.0
86	108.5
87	53.0
88	63.5
89	50.0
90	26.0
91	15.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1350546.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.0698653615605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.6788507722759	12.048778559186097
2	12.743693477529558	4.605536506959116
3	5.861283733323706	3.1773782372119217
4	3.169706178094187	2.2910465549547387
5	1.8985840272019687	1.7153578874574447
6	1.335272116794092	1.4476912422909076
7	0.9320666603326213	1.1789623342146864
8	0.6882556682321618	0.9949349807428812
9	0.5209434282153087	0.8472039847956349
>10	3.661050035172933	14.16482906093188
>50	1.0944619119275707	14.550746890371592
>100	1.4023049336621463	37.9886745381031
>500	0.006558573206253824	0.7353702784398621
>1k	0.005328840730081231	2.466319919541911
>5k	0.001639643301563456	1.7871690247982468
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT	6979	0.5167539646927983	Illumina Single End Adapter 2 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	5798	0.4293078503064687	No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	5749	0.4256796880669003	Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	5602	0.4147952013481955	No Hit
TTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	4908	0.3634085769755343	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	4746	0.35141342834675754	No Hit
GGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	4352	0.3222400421755349	No Hit
ATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	4109	0.30424731923236975	No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	3164	0.23427561889783835	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	2052	0.15193854929783954	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1945	0.1440158276726598	No Hit
CTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1843	0.1364633266841707	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	1685	0.12476435456474641	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5450388213359634	0.0
2	0.0	0.0	0.0	1.2546036936172482	0.0
3	0.0	0.0	0.0	4.5864413355783515	0.0
4	0.0	0.0	0.0	5.335249595348844	0.0
5	0.0	0.0	0.0	6.391785248336599	0.0
6	0.0	0.0	0.0	9.809440033882593	0.0
7	0.0	0.0	0.0	10.202096041156688	0.0
8	0.0	0.0	0.0	10.988148496978258	0.0
9	0.0	0.0	0.0	11.471508560241562	0.0
10	7.404412733812843E-5	0.0	0.0	13.107661642032186	0.0
11	7.404412733812843E-5	0.0	0.0	14.576326907783963	0.0
12	7.404412733812843E-5	0.0	0.0	15.158906101680357	0.0
13	7.404412733812843E-5	0.0	0.0	15.44560496273359	0.0
14	7.404412733812843E-5	0.0	0.0	15.603318953963804	0.0
15	7.404412733812843E-5	0.0	0.0	15.867434356178908	0.0
16	0.0028877209661870087	0.0	0.0	16.519466941518466	0.0
17	0.0028877209661870087	0.0	0.0	17.43317147287097	0.0
18	0.0028877209661870087	0.0	0.0	18.43143439764362	0.0
19	0.0028877209661870087	0.0	0.0	18.984025719968074	0.0
20	0.0028877209661870087	0.0	0.0	19.734759127049358	0.0
21	0.0028877209661870087	0.0	0.0	20.733762493095384	0.0
22	0.0028877209661870087	0.0	0.0	21.511744139037102	0.0
23	0.0028877209661870087	0.0	0.0	22.33355990836299	0.0
24	0.002961765093525137	0.0	0.0	22.93479822234859	0.0
25	0.002961765093525137	0.0	0.0	23.470803660149304	0.0
26	0.002961765093525137	0.0	0.0	23.98570652165865	0.0
27	0.002961765093525137	0.0	0.0	24.46751165824785	0.0
28	0.002961765093525137	0.0	0.0	25.03202408507374	0.0
29	0.002961765093525137	0.0	0.0	25.515236060082366	0.0
30	0.002961765093525137	0.0	0.0	26.036802893052144	0.0
31	0.002961765093525137	0.0	0.0	26.533564943363647	0.0
32	0.002961765093525137	0.0	0.0	27.032844494004646	0.0
33	0.002961765093525137	0.0	0.0	27.544859634547805	0.0
34	0.002961765093525137	0.0	0.0	28.036512640072978	0.0
35	0.002961765093525137	0.0	0.0	28.57807138742405	0.0
36	0.002961765093525137	0.0	0.0	29.090826969240588	0.0
37	0.002961765093525137	0.0	0.0	29.597436888488062	0.0
38	0.002961765093525137	0.0	0.0	30.091459306088055	0.0
39	0.002961765093525137	0.0	0.0	30.57008054520172	0.0
40	0.002961765093525137	0.0	0.0	31.031893767409624	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	35	1.01974365E-7	46.000004	24
GCGAACC	35	1.01974365E-7	46.000004	24
CTGCGTA	35	1.01974365E-7	46.000004	27
CGGTACA	35	1.01974365E-7	46.000004	28
ACGGTAC	35	1.01974365E-7	46.000004	27
TCACGAC	20	6.311849E-4	46.0	16
AACCGTT	25	3.417476E-5	46.0	33
AACCGTA	20	6.311849E-4	46.0	24
ACACGTT	30	1.8616465E-6	46.0	37
GATCGAG	25	3.417476E-5	46.0	43
CGAGTGT	20	6.311849E-4	46.0	24
CAACGTA	20	6.311849E-4	46.0	15
CAACGCG	20	6.311849E-4	46.0	44
CGAAACG	20	6.311849E-4	46.0	46
CATGCGG	45	3.110472E-10	46.0	2
ACGCACT	20	6.311849E-4	46.0	44
GACACGT	30	1.8616465E-6	46.0	9
GCAACGA	45	3.110472E-10	46.0	9
GGCGTAC	20	6.311849E-4	46.0	38
TAGAACG	30	1.8616465E-6	46.0	18
>>END_MODULE