FastQCFastQC Report
Thu 26 May 2016
SRR1545670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545670_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2406208
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC92080.38267680931989256Illumina Single End Adapter 2 (95% over 21bp)
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA65290.27133980104795596No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63650.26452409766736706No Hit
ATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG42030.17467317871106738No Hit
ATGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT38570.16029370694470305No Hit
ATTGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC36100.15002859270686492No Hit
ATGCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC35620.14803375269303404No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT33030.13726992845173816Illumina Single End Adapter 2 (95% over 22bp)
ATGTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC30310.12596583504002978No Hit
ATATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC28160.11703061414474558No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC26300.10930060909115089Illumina Single End Adapter 2 (95% over 21bp)
ATGGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC24750.10285893821315531No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC351.2126657E-745.0000042
CCTTACG207.034828E-445.037
CGTAGAT253.892082E-545.031
ACGTATG207.034828E-445.03
ACGTATC207.034828E-445.012
ACGGGTA1650.040.909097
CGCGAAT501.0822987E-940.54
CGGTAAG501.0822987E-940.53
CGCCCGT501.0822987E-940.525
TACACAA20200.039.98762532
ATACGTA1250.039.61
ATTACGT2450.039.4897961
ATCGGTT3850.039.155841
ATTGTTT44350.039.1149941
TCGTTGC1850.038.918922
GGACTAA25050.038.35329410
GCATAGA8850.038.13559340
ACTAATC24600.037.95731712
CACGTTA301.1402929E-437.50000412
CCGTTTA301.1402929E-437.50000442